Cloning and Characterisation of a novel phosphate transporter gene <i>EcPT1</i> from <i>Eichhornia crassipes</i>

Eichhornia crassipes (Mart.) Solms can absorb nitrogen and phosphorus efficiently from water. Cloning the phosphate transporter 1 from E. crassipes (EcPHT1) and studying its characteristics can help to explain the molecular mechanism of absorption and transportation of inorganic phosphate (Pi) in E. crassipes and develop new genetic resources for phosphate transport to aid genetic breeding of crops with increased phosphate use efficiency. In this paper, we have used RACE method to clone the EcPT1 with 1572 bps which encoded a polypeptide of 523 amino acids and contained 11 transmembrane regions which was the typical structure of plant PHT1. The phylogenetic analysis also showed EcPT1 was closely related to other plant PHT1s. Quantitative real-time RT-PCR (qRT-PCR) showed that the EcPT1 expression in roots was up-regulated under low phosphorus stress . Moreover the EcPT1 expression in leaves was suppressed after E. crassipes was transferred to sufficient Pi from deficient Pi. Yeast functional complementation tests showed that the EcPT1 could complement the phosphate transport function of the Pi deficient yeast PHO84 in 175 μM Pi and make it grow best in pH = 5 or 6. All these results indicated that EcPT1 might be a phosphate transporter gene. Its expression was different in different tissues.</p

Raw data ( 1H NMR specra) obtained from polar metabolite extraction of Peganum harmala L. from NMR-based metabolomic profiling of <i>Peganum harmala</i> L. reveals dynamic variations between different growth stages

variable bins were normalized to the whole spectral area and 20 samples data were listed in this file

Supplementary figures and tables from "NMR-Based Metabolomic Profiling Of Peganum Harmala L. Reveals Dynamic Variations Derived From Different Growth Stages" from NMR-based metabolomic profiling of <i>Peganum harmala</i> L. reveals dynamic variations between different growth stages

Plot design for sampling;Xinjiang changji region climate data in 2014;Identification and relative quantification of principal metabolites;1D and 2D NMR spectra of samples; Permutation test model validation plots

Cembranoids from the Soft Coral Sinularia rigida with Antifouling Activities

Chemical examination of the soft coral Sinularia rigida resulted in the isolation of 12 new cembranoids, namely, sinulariols T–Z₅ (<b>1</b>–<b>12</b>), together with a known analogue, <b>13</b>. Their structures were determined on the basis of 1D and 2D NMR (COSY, HSQC, HMBC, and NOESY) spectroscopic analyses in association with MS and IR data. Compounds <b>7</b> and <b>13</b> showed potent antifouling activity for the inhibition against the barnacle Balanus amphitrite and moderate inhibition against Bugula neritina. The primary structure–activity relationship is discussed

DataSheet1_Selective synthesis of rebaudioside M2 through structure-guided engineering of glycosyltransferase UGT94D1.docx

Rebaudioside M2 (Reb M2), a novel steviol glycoside derivative, has limited industrial applications due to its low synthetic yield and selectivity. Herein, we identify UGT94D1 as a selective glycosyltransferase for rebaudioside D (Reb D), leading to the production of a mono β-1,6-glycosylated derivative, Reb M2. A variant UGT94D1-F119I/D188P was developed through protein engineering. This mutant exhibited a 6.33-fold improvement in catalytic efficiency, and produced Reb M2 with 92% yield. Moreover, molecular dynamics simulations demonstrated that UGT94D1-F119I/D188P exhibited a shorter distance between the nucleophilic oxygen (OH6) of the substrate Reb D and uridine diphosphate glucose, along with an increased Ophosphate-C1-Oacceptor angle, thus improving the catalytic activity of the enzyme. Therefore, this study provides an efficient method for the selective synthesis of Reb M2 and paves the way for its applications in various fields.</p

Fluorophore Label-Free Light-up Near Infrared Deoxyribonucleic Acid Nanosensor for Monitoring Extracellular Potassium Levels

Fluorescent DNA nanosensors have been widely used due to their unique advantages, among which the near-infrared (NIR) imaging mode can provide deeper penetration depth and lower biological background for the nanosensors. However, efficient NIR quenchers require ingenious design, complex synthesis, and modification, which severely limit the development of NIR DNA nanosensors. Label-free strategies based on G-quadruplex (G4) and NIR G4 dyes were first introduced into in situ extracellular imaging, and a novel NIR sensing strategy for the specific detection of extracellular targets is proposed. The strategy avoids complex synthesis and site-specific modification by controlling the change of the NIR signal through the formation of a G4 nanostructure. A light-up NIR DNA nanosensor based on potassium ion (K+)-sensitive G4 chain PS2.M was constructed to verify the strategy. PS2.M forms a stable G4 nanostructure in the presence of K+ and activates the NIR G4 dye CSTS, thus outputting NIR signals. The nanosensor can rapidly respond to K+ with a linear range of 5–50 mM and has good resistance to interference. The nanosensor with cholesterol can provide feedback on the changes in extracellular K+ concentration in many kinds of cells, serving as a potential tool for the study of diseases such as epilepsy and cancer, as well as the development of related drugs. The strategy can be potentially applied to the NIR detection of a variety of extracellular targets with the help of functional DNAs such as aptamer and DNAzyme