Explanation of environmental regulation, R&D investment and green technology innovation index system.

Explanation of environmental regulation, R&D investment and green technology innovation index system.</p

Test results of panel smoothness.

Test results of panel smoothness.</p

Impulse response in eastern China.

Notes: The horizontal axis represents the number of lag periods (years), the middle curve is the impulse response function curve, and the upper and lower curves represent the 95% confidence interval.</p

Additional file 1: of Targeting of HER3 with Functional Cooperative miRNAs Enhances Therapeutic Activity in HER2-Overexpressing Breast Cancer Cells

Figure S1. Transfection with the multi-miRNA (cluster) lentiviral vector significantly enhanced T-DM1-induced growth inhibitory effects on BT474 cells. The stable clones of BT474 cells transfected with empty vector (pCDH), pCDH-miR-125a, or pCDH-miR-205 alone or pCDH-miR-125a-miR-205 (cluster) were seeded onto 96-well plates. After 24 hrs, the 96-well plates were then placed into the IncuCyte system to measure cell proliferation in a real time. Data show growth curves of the clones in comparison with their responses to the treatment of T-DM1 (10 Îźg/ml) in the indicated time period. (TIF 2615 kb

3D Flower-like Micro/Nano Ce–Mo Composite Oxides as Effective Bifunctional Catalysts for One-Pot Conversion of Fructose to 2,5-Diformylfuran

Three-dimensional flower-like Ce–Mo micro/nano composite oxides with different Ce/Mo atomic ratios (f-Ce_{10‑<i>x</i>}Mo_<i>x</i>O_δ) were synthesized as effective bifunctional catalysts for one-pot conversion of fructose to 2,5-diformylfuran (DFF) via dehydration and aerobic oxidation. The catalytic activities of f-Ce_{10‑<i>x</i>}Mo_<i>x</i>O_δ depended on their structure and physicochemical properties, which were closely related to their elementary compositions. The f-Ce₉Mo₁O_δ, which possesses polymeric octahedral MoO_<i>z</i> species, the highest O_α ratio, BET surface area, and acidity density, exhibited the highest catalytic activity for DFF production. High DFF yields of 94% and 74% were obtained from f-Ce₉Mo₁O_δ catalyzed HMF oxidation and “one-pot” fructose conversion, respectively. Furthermore, f-Ce₉Mo₁O_δ is easily prepared, has low cost, and is ecofriendly, which has potential application in industrial production of DFF from fructose

Depletion of JMJD5 sensitizes tumor cells to microtubule-destabilizing agents by altering microtubule stability

<p>Microtubules play essential roles in mitosis, cell migration, and intracellular trafficking. Drugs that target microtubules have demonstrated great clinical success in cancer treatment due to their capacity to impair microtubule dynamics in both mitotic and interphase stages. In a previous report, we demonstrated that JMJD5 associated with mitotic spindle and was required for proper mitosis. However, it remains elusive whether JMJD5 could regulate the stability of cytoskeletal microtubules and whether it affects the efficacy of microtubule-targeting agents. In this study, we find that JMJD5 localizes not only to the nucleus, a fraction of it also localizes to the cytoplasm. JMJD5 depletion decreases the acetylation and detyrosination of α-tubulin, both of which are markers of microtubule stability. In addition, microtubules in JMJD5-depleted cells are more sensitive to nocodazole-induced depolymerization, whereas JMJD5 overexpression increases α-tubulin detyrosination and enhances the resistance of microtubules to nocodazole. Mechanistic studies revealed that JMJD5 regulates MAP1B protein levels and that MAP1B overexpression rescued the microtubule destabilization induced by JMJD5 depletion. Furthermore, JMJD5 depletion significantly promoted apoptosis in cancer cells treated with the microtubule-targeting anti-cancer drugs vinblastine or colchicine. Together, these findings suggest that JMJD5 is required to regulate the stability of cytoskeletal microtubules and that JMJD5 depletion increases the susceptibility of cancer cells to microtubule-destabilizing agents.</p

The distance parameters of different drug-protein systems at 298 K.

<p>The distance parameters of different drug-protein systems at 298 K.</p

TCRP1 regulates the PI3K/Akt/NFκB pathway and protects oral squamous cells from cisplatin induced apoptosis.

<p>TCRP1-over-expressing cells (Tca/TCRP1) were treated with 30 µM DDP in the presence or absence of specific inhibitors of PI3K (LY2294002 [50 µM]), NFκB (Bay11-7082 [20 µM]) or vehicle (DMSO) and cultured for an additional 24 h. <b>A.</b> The degree of apoptosis was determined by flow cytometry as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051413#pone-0051413-g006" target="_blank">Figure 6</a>. Significantly higher percentages of apoptotic cells were found in the presence of both inhibitors (*<i>P</i><0.05, compared with control). <b>B.</b> Western blot analyses showing the effect of TCRP1 on apoptosis associated proteins in Tca/TCRP1 cells. Nuclear histone and β-actin were included as loading controls. All experiments were repeated at least three times, and representative results are shown.</p

Identification of genes potentially involved in TCRP1-mediated multidrug-resistance phenotype.

<p><b>A.</b> Differentially expressed genes in the Tca/PYM-Con (left) and Tca/PYM-siTCRP1 (right) cells were analyzed by Human Toxicology and Drug Resistance Microarray (OHS-401). This microarray included 263 key genes critical in drug metabolism and resistance. <b>B.</b> A heat map generated from the microarray shows gene expression as Tca/PYM-siTCRP1 over Tca/PYM-Con cells for the genes whose expressions had increased or decreased by more than 1.5-fold in TCRP1 knockdown cells. <b>C.</b> GO analysis of functional gene grouping of the differentially expressed genes involved in TCRP1-associated multidrug-resistance phenotype.</p

Oriented Enzyme Immobilization at the Oil/Water Interface Enhances Catalytic Activity and Recyclability in a Pickering Emulsion

Enzyme-loaded water-in-oil Pickering emulsion is a promising system for biphasic catalytic reactions. In this paper, we report on oriented enzyme immobilization at the oil/water interface in a Pickering emulsion, in which CHO-Janus silica nanoparticles (CHO-JNPs) are utilized as a stabilizer of the emulsion and support for the enzyme to enhance both catalytic activity and recyclability. The catalytic performance of this immobilized enzyme (lipase from Candida sp.) was evaluated by esterification of hexanoic acid and 1-hexanol in a water/heptane biphasic medium. The results show that the specific catalytic activity of the immobilized enzyme (33.2 U mL^–1) was 6.5 and 1.4 times higher than that of free enzyme (5.1 U mL^–1) and encapsulated enzyme in the liquid core (23.3 U mL^–1), respectively. Moreover, the immobilized enzyme demonstrated good stability and recyclability, retaining 75% of its activity after 9 cycles. We expect that oriented enzyme immobilization at the oil/water interface will be an important strategy for enhancing catalytic performance in Pickering emulsions