6 research outputs found
Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ² Π² Π²ΠΈΡΠ°ΠΌΠΈΠ½Π½ΡΡ ΡΠ±ΠΎΡΠ°Ρ
Introduction. Vitamin preparations are widely used in the therapy of various diseases. The main dosage forms of the preparations include tinctures or decoctions. In addition to vitamins, medicinal plant raw materials, the components of the preparations, contain flavonoids. Objective: to develop methods for quantification of flavonoids in vitamin preparations. Material and methods. The investigation objects were model samples of vitamin preparations Nos. 1 and 2 and commercial samples of mountain ash (Sorbus aucuparia) berries, rose (RΕsa) hips, and black currants (Ribes nigrum). The amount of flavonoids was determined spectrophotometrically. Results. Optimal pH values and an optimal extractant for flavonoid-aluminum chloride complexation in the vitamin preparations were found to be 1.5-2.3 and 95% ethanol, respectively. It was ascertained that the amount of flavonoids in black currants and mountain ash berries should be calculated with reference to rutin and that in rose hips to luteolin. Conclusion. A spectrophotometric procedure was developed to determine the amount of flavonoids in vitamin preparations Nos. 1 and 2 and in their components: rose hips, mountain ash berries and black currants. Standardization of vitamin preparations Nos. 1 and 2 and their components was shown to be unreasonable in terms of the amount of flavonoids.ΠΠ²Π΅Π΄Π΅Π½ΠΈΠ΅. ΠΠΈΡΠ°ΠΌΠΈΠ½Π½ΡΠ΅ ΡΠ±ΠΎΡΡ ΡΠΈΡΠΎΠΊΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΡΡΡΡ Π² ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ. ΠΡΠ½ΠΎΠ²Π½ΠΎΠΉ Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΡΠΎΡΠΌΠΎΠΉ ΡΠ±ΠΎΡΠΎΠ² ΡΠ²Π»ΡΡΡΡΡ Π½Π°ΡΡΠΎΠΈ ΠΈΠ»ΠΈ ΠΎΡΠ²Π°ΡΡ. ΠΠΎΠΌΠΈΠΌΠΎ Π²ΠΈΡΠ°ΠΌΠΈΠ½ΠΎΠ², Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΠΎΠ΅ ΡΠ°ΡΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ΅ ΡΡΡΡΠ΅ - ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΡ ΡΠ±ΠΎΡΠ° - ΡΠΎΠ΄Π΅ΡΠΆΠ°Ρ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄Ρ. Π¦Π΅Π»Ρ - ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠ° ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΡΡΠΌΠΌΡ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ² Π² Π²ΠΈΡΠ°ΠΌΠΈΠ½Π½ΡΡ
ΡΠ±ΠΎΡΠ°Ρ
. ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠ±ΡΠ΅ΠΊΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ - ΠΌΠΎΠ΄Π΅Π»ΡΠ½ΡΠ΅ ΠΎΠ±ΡΠ°Π·ΡΡ Π²ΠΈΡΠ°ΠΌΠΈΠ½Π½ΡΡ
ΡΠ±ΠΎΡΠΎΠ² β 1 ΠΈ β 2, ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΡΠ΅ ΠΎΠ±ΡΠ°Π·ΡΡ ΠΏΠ»ΠΎΠ΄ΠΎΠ² ΡΡΠ±ΠΈΠ½Ρ ΠΎΠ±ΡΠΊΠ½ΠΎΠ²Π΅Π½Π½ΠΎΠΉ, ΡΠΈΠΏΠΎΠ²Π½ΠΈΠΊΠ° ΠΈ ΡΠΌΠΎΡΠΎΠ΄ΠΈΠ½Ρ ΡΠ΅ΡΠ½ΠΎΠΉ. Π‘ΡΠΌΠΌΡ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ² ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠ»Ρ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ° ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ² Π²ΠΈΡΠ°ΠΌΠΈΠ½Π½ΡΡ
ΡΠ±ΠΎΡΠΎΠ² Ρ Π°Π»ΡΠΌΠΈΠ½ΠΈΡ Ρ
Π»ΠΎΡΠΈΠ΄ΠΎΠΌ Π²ΡΡΠ²Π»Π΅Π½Ρ: ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΠΎΠ΅ Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅ ΡΠ - 1,5-2,3, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΠΉ ΡΠΊΡΡΡΠ°Π³Π΅Π½Ρ - 95% ΡΡΠΈΠ»ΠΎΠ²ΡΠΉ ΡΠΏΠΈΡΡ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π² ΠΏΠ»ΠΎΠ΄Π°Ρ
ΡΠΌΠΎΡΠΎΠ΄ΠΈΠ½Ρ ΡΠ΅ΡΠ½ΠΎΠΉ ΠΈ ΡΡΠ±ΠΈΠ½Ρ ΠΎΠ±ΡΠΊΠ½ΠΎΠ²Π΅Π½Π½ΠΎΠΉ ΠΏΠ΅ΡΠ΅ΡΡΠ΅Ρ ΡΡΠΌΠΌΡ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ² ΡΠ»Π΅Π΄ΡΠ΅Ρ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΡΡ Π½Π° ΡΡΡΠΈΠ½, Π° Π² ΠΏΠ»ΠΎΠ΄Π°Ρ
ΡΠΈΠΏΠΎΠ²Π½ΠΈΠΊΠ° - Π½Π° Π»ΡΡΠ΅ΠΎΠ»ΠΈΠ½. ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π° ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠ° ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΡΡΠΌΠΌΡ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ² Π² Π²ΠΈΡΠ°ΠΌΠΈΠ½Π½ΡΡ
ΡΠ±ΠΎΡΠ°Ρ
β 1 ΠΈ β 2 ΠΈ ΠΈΡ
ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠ°Ρ
: ΠΏΠ»ΠΎΠ΄Π°Ρ
ΡΠΈΠΏΠΎΠ²Π½ΠΈΠΊΠ°, ΡΡΠ±ΠΈΠ½Ρ ΠΎΠ±ΡΠΊΠ½ΠΎΠ²Π΅Π½Π½ΠΎΠΉ ΠΈ ΡΠΌΠΎΡΠΎΠ΄ΠΈΠ½Ρ ΡΠ΅ΡΠ½ΠΎΠΉ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΡΡΠ°Π½Π΄Π°ΡΡΠΈΠ·Π°ΡΠΈΡ Π²ΠΈΡΠ°ΠΌΠΈΠ½Π½ΡΡ
ΡΠ±ΠΎΡΠΎΠ² β 1 ΠΈ β 2 ΠΈ ΠΈΡ
ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ² ΠΏΠΎ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Ρ Β«ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΡΡΠΌΠΌΡ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ²Β» Π½Π΅ ΡΠ°ΡΠΈΠΎΠ½Π°Π»ΡΠ½Π°
Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ² Π² Π²ΠΈΡΠ°ΠΌΠΈΠ½Π½ΡΡ ΡΠ±ΠΎΡΠ°Ρ
Introduction. Vitamin preparations are widely used in the therapy of various diseases. The main dosage forms of the preparations include tinctures or decoctions. In addition to vitamins, medicinal plant raw materials, the components of the preparations, contain flavonoids. Objective: to develop methods for quantification of flavonoids in vitamin preparations. Material and methods. The investigation objects were model samples of vitamin preparations Nos. 1 and 2 and commercial samples of mountain ash (Sorbus aucuparia) berries, rose (RΕsa) hips, and black currants (Ribes nigrum). The amount of flavonoids was determined spectrophotometrically. Results. Optimal pH values and an optimal extractant for flavonoid-aluminum chloride complexation in the vitamin preparations were found to be 1.5-2.3 and 95% ethanol, respectively. It was ascertained that the amount of flavonoids in black currants and mountain ash berries should be calculated with reference to rutin and that in rose hips to luteolin. Conclusion. A spectrophotometric procedure was developed to determine the amount of flavonoids in vitamin preparations Nos. 1 and 2 and in their components: rose hips, mountain ash berries and black currants. Standardization of vitamin preparations Nos. 1 and 2 and their components was shown to be unreasonable in terms of the amount of flavonoids.ΠΠ²Π΅Π΄Π΅Π½ΠΈΠ΅. ΠΠΈΡΠ°ΠΌΠΈΠ½Π½ΡΠ΅ ΡΠ±ΠΎΡΡ ΡΠΈΡΠΎΠΊΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΡΡΡΡ Π² ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ. ΠΡΠ½ΠΎΠ²Π½ΠΎΠΉ Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΡΠΎΡΠΌΠΎΠΉ ΡΠ±ΠΎΡΠΎΠ² ΡΠ²Π»ΡΡΡΡΡ Π½Π°ΡΡΠΎΠΈ ΠΈΠ»ΠΈ ΠΎΡΠ²Π°ΡΡ. ΠΠΎΠΌΠΈΠΌΠΎ Π²ΠΈΡΠ°ΠΌΠΈΠ½ΠΎΠ², Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΠΎΠ΅ ΡΠ°ΡΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ΅ ΡΡΡΡΠ΅ - ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΡ ΡΠ±ΠΎΡΠ° - ΡΠΎΠ΄Π΅ΡΠΆΠ°Ρ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄Ρ. Π¦Π΅Π»Ρ - ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠ° ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΡΡΠΌΠΌΡ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ² Π² Π²ΠΈΡΠ°ΠΌΠΈΠ½Π½ΡΡ
ΡΠ±ΠΎΡΠ°Ρ
. ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠ±ΡΠ΅ΠΊΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ - ΠΌΠΎΠ΄Π΅Π»ΡΠ½ΡΠ΅ ΠΎΠ±ΡΠ°Π·ΡΡ Π²ΠΈΡΠ°ΠΌΠΈΠ½Π½ΡΡ
ΡΠ±ΠΎΡΠΎΠ² β 1 ΠΈ β 2, ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΡΠ΅ ΠΎΠ±ΡΠ°Π·ΡΡ ΠΏΠ»ΠΎΠ΄ΠΎΠ² ΡΡΠ±ΠΈΠ½Ρ ΠΎΠ±ΡΠΊΠ½ΠΎΠ²Π΅Π½Π½ΠΎΠΉ, ΡΠΈΠΏΠΎΠ²Π½ΠΈΠΊΠ° ΠΈ ΡΠΌΠΎΡΠΎΠ΄ΠΈΠ½Ρ ΡΠ΅ΡΠ½ΠΎΠΉ. Π‘ΡΠΌΠΌΡ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ² ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠ»Ρ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ° ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ² Π²ΠΈΡΠ°ΠΌΠΈΠ½Π½ΡΡ
ΡΠ±ΠΎΡΠΎΠ² Ρ Π°Π»ΡΠΌΠΈΠ½ΠΈΡ Ρ
Π»ΠΎΡΠΈΠ΄ΠΎΠΌ Π²ΡΡΠ²Π»Π΅Π½Ρ: ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΠΎΠ΅ Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅ ΡΠ - 1,5-2,3, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΠΉ ΡΠΊΡΡΡΠ°Π³Π΅Π½Ρ - 95% ΡΡΠΈΠ»ΠΎΠ²ΡΠΉ ΡΠΏΠΈΡΡ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π² ΠΏΠ»ΠΎΠ΄Π°Ρ
ΡΠΌΠΎΡΠΎΠ΄ΠΈΠ½Ρ ΡΠ΅ΡΠ½ΠΎΠΉ ΠΈ ΡΡΠ±ΠΈΠ½Ρ ΠΎΠ±ΡΠΊΠ½ΠΎΠ²Π΅Π½Π½ΠΎΠΉ ΠΏΠ΅ΡΠ΅ΡΡΠ΅Ρ ΡΡΠΌΠΌΡ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ² ΡΠ»Π΅Π΄ΡΠ΅Ρ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΡΡ Π½Π° ΡΡΡΠΈΠ½, Π° Π² ΠΏΠ»ΠΎΠ΄Π°Ρ
ΡΠΈΠΏΠΎΠ²Π½ΠΈΠΊΠ° - Π½Π° Π»ΡΡΠ΅ΠΎΠ»ΠΈΠ½. ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π° ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠ° ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΡΡΠΌΠΌΡ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ² Π² Π²ΠΈΡΠ°ΠΌΠΈΠ½Π½ΡΡ
ΡΠ±ΠΎΡΠ°Ρ
β 1 ΠΈ β 2 ΠΈ ΠΈΡ
ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠ°Ρ
: ΠΏΠ»ΠΎΠ΄Π°Ρ
ΡΠΈΠΏΠΎΠ²Π½ΠΈΠΊΠ°, ΡΡΠ±ΠΈΠ½Ρ ΠΎΠ±ΡΠΊΠ½ΠΎΠ²Π΅Π½Π½ΠΎΠΉ ΠΈ ΡΠΌΠΎΡΠΎΠ΄ΠΈΠ½Ρ ΡΠ΅ΡΠ½ΠΎΠΉ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΡΡΠ°Π½Π΄Π°ΡΡΠΈΠ·Π°ΡΠΈΡ Π²ΠΈΡΠ°ΠΌΠΈΠ½Π½ΡΡ
ΡΠ±ΠΎΡΠΎΠ² β 1 ΠΈ β 2 ΠΈ ΠΈΡ
ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ² ΠΏΠΎ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Ρ Β«ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΡΡΠΌΠΌΡ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ²Β» Π½Π΅ ΡΠ°ΡΠΈΠΎΠ½Π°Π»ΡΠ½Π°
Qualitative and quantitative contents of organic acids in multivitamin plant raw materials studied using various physicochemical
Chemical composition and content of polysaccharides from the yellow iris (Iris pseudacorus L.) rhizomes
Background: The aim of the present study was to examine the composition and content of the polysaccharide complex and polysaccharide fractions from yellow iris (Iris pseudacorus L.) rhizomes growing in Russian Federation (Moscow region). Materials and Methods:Traditional pharmacognostic methods were used in the analysis of polysaccharides as biologically active substances. The total amount of polysaccharides was determined by a gravimetric procedure with alcohol precipitation. Fractions of polysaccharides were isolated by sequential changing of solvents.The content of monosaccharides was determined by the method of high-performance liquid chromatography with refractometric detection (HPLC-RID) after acid hydrolysis. HPLC-RID procedure was performed using Chromatograph Agilent 1260 Infinity, Sugar-Pak column (300Γ6.5 mm), and isocratic elution mode. Results: Polysaccharide complex was isolated, the total content of polysaccharides in the rhizomes of I. pseudacorus was determined, as well as their distribution by fractions (water-soluble polysaccharides, pectic substances, hemicellulose A, hemicellulose B), the composition of individual sugars (glucose, xylose, galactose, mannose, rhamnose, arabinose) was determined for each fraction. Conclusion: In our experiment polysaccharide complex was first identified and described in detail for I. pseudacorus grown in Moscow region. Β© 2020 Phcogj.Com. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license
Composition of biologically active compounds, biological and pharmacological activity of the three-part beggarticks (Bidens tripartita L.)
This reviewcontains the analysis data on phytochemical composition ofthree-part beggarticks(Bidens tripartitaL.) and full biological activity spectrum of themedicinalraw material. Flavonoids, essentialoils, phenolcarboxylic acids, polysaccharides, aromatic hydroxy aldehydes, coumarins, tannins, and polyacetylenes are biologically active substances (BAS)ofB. tripartita.Various BAS cause multiple pharmacological and biological effects of theplant. Extracts possess antimycotic, antiinflammatory, antithrombotic, antiradical, antioxidant, antibacterial, antidiabetic, antiulcerogenic, and antitumor effects. The extracts are also ofanalgesic, antipyretic, antiallergic, hepatoprotective, immunostimulating,and hypotensive pharmacological effects. There is also a possibility to inhibit the main enzymes as well as to protect DNA. The B. tripartita medicinal raw material is of great importance for development and making of new drugs