799 research outputs found
A permeability model for the hydraulic fracture filled with proppant packs under combined effect of compaction and embedment
The authors acknowledge the financial support from Science Foundation of China University of Petroleum, Beijing (No. 2462014YJRC060 and No.2462014YJRC059)Peer reviewedPostprin
Aromadendrin protects mouse liver from sepsis-induced injury by inhibiting NF-κB signaling pathway
Purpose: To investigate the protective role of aromadendrin in septic liver injury in mice, and its mechanism of action.Methods: Eight-week-old male C57BL/6 mice (n=6 for each group) were administrated with aromadendrin (SMB00175, Sigma-Aldrich) at 0 mg/kg, 30 mg/kg and 60 mg/kg via a hypodermic intraperitoneal injection. HE staining was used to examine liver histopathological structural changes in the liver while DAPI/Tunel staining was employed to evaluate liver cell apoptosis. The mRNA expression levels of TNF-α, IL-1β and IL-6 were determined by quantitative reverse transcriptionpolymerase chain reaction (qRT-PCR). Moreover, enzyme-linked immunosorbent assay (ELISA)was applied to assess the levels of TNF-α, IL-1β and IL-6, as well as the activities of catalase (CAT), antioxidant glutathione (GSH), superoxide dismutase (SOD) and malondialdehyde (MDA). Moreover, the protein levels of p65, p-p65, p-IκBα and IκBα were analyzed by Western blotting.Results: The liver tissues exhibited severe structural damages, with edema, necrosis, and neutrophil infiltration, but recovered as a result of aromadendrin treatment (p < 0.05). The increased serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in CLP mice were reduced by aromadendrin, which also attenuated liver injury and cell apoptosis. Aromadendrin inhibited the levels ofu TNF-α, IL-1β and IL-6 in the mice, while the activities of GSH and antioxidant enzymes (SOD and CAT) were also significantly lowered in the mice, but attenuated by aromadendrin (p < 0.05). Aromadendrin also prevented the increased level of MDA, and suppressed the phosphorylation of p65 and IκBα (p < 0.05).Conclusion: Aromadendrin protects mouse liver from sepsis-induced injury by inhibiting NF-κB signaling in vivo, thus suggesting a potential strategy for the therapy of sepsis-induced liver injury
Generalized Hilbert Operator Acting on Bloch Type Spaces
Let be a positive Borel measure on the interval [0,1). For ,
the Hankel matrix
with entries
formally induces the operator
on the space of all analytic functions
in the unit disc . In this
paper, we characterize the measures for which
() is a bounded (resp., compact) operator from the Bloch type
space () into .
We also give a necessary condition for which is a
bounded operator by acting on Bloch type spaces for general cases
Hyper Association Graph Matching with Uncertainty Quantification for Coronary Artery Semantic Labeling
Coronary artery disease (CAD) is one of the primary causes leading to death
worldwide. Accurate extraction of individual arterial branches on invasive
coronary angiograms (ICA) is important for stenosis detection and CAD
diagnosis. However, deep learning-based models face challenges in generating
semantic segmentation for coronary arteries due to the morphological similarity
among different types of coronary arteries. To address this challenge, we
propose an innovative approach using the hyper association graph-matching
neural network with uncertainty quantification (HAGMN-UQ) for coronary artery
semantic labeling on ICAs. The graph-matching procedure maps the arterial
branches between two individual graphs, so that the unlabeled arterial segments
are classified by the labeled segments, and the coronary artery semantic
labeling is achieved. By incorporating the anatomical structural loss and
uncertainty, our model achieved an accuracy of 0.9345 for coronary artery
semantic labeling with a fast inference speed, leading to an effective and
efficient prediction in real-time clinical decision-making scenarios.Comment: 8 pages, 2 figure
Ticking terahertz wave generation in attoseconds
We perform a joint measurement of terahertz waves and high-order harmonics
generated from noble atoms driven by a fundamental laser pulse and its second
harmonic. By correlating their dependence on the phase-delay of the two pulses,
we determine the generation of THz waves in tens of attoseconds precision.
Compared with simulations and models, we find that the laser-assisted
soft-collision of the electron wave packet with the atomic core plays a key
role. It is demonstrated that the rescattering process, being indispensable in
HHG processes, dominant THz wave generation as well but in a more elaborate
way. The new finding might be helpful for the full characterization of the
rescattering dynamics.Comment: 4 figure
Construction of retrovirus vector taking MDR1/ACBC1 and its transfection into human placenta derived mesenchymal stem cells
In the study, we used both the methods of perfusion and density gradient centrifugation to isolate and purify mesenchymal stem cells (MSCS) from placenta tissue, and constructed a retroviral vector with multiple drug resistant genes, and the green fluorescent protein (GFP) has been used as an indicative mark. The 293T cell was transfected by the retroviral vector PMX-flag-MDR1-GFP together with its peripheral membrane protein gene. After the infective and replication–defective retrovirus were acquired, we transfected them into human placenta-derived mesenchymal stem cells (HPMSCs). We successfully observed the expression of the reporter gene-GFP by using the green light fluorescence microscope and the p-glycoprotein (P-gp) expressed by exogenous gene MDR1 by Western Blotting. All these facts indicated that the retroviral vector PMX-flag-MDR1-GFP had successfully been transfected into HPMSCs and the exogenous gene multidrug resistance (MDR)1 was detected as normally expressed. The daunorubicin (DNR) pump experiment proved that P-gp of HPMSCs transfected with PMX-flag-MDR1-GFP was of biological activity. The result indicates that MDR1 retroviral vector can transfect the HPMSCs. Not only can the exogenous gene be expressed, but also the expression protein had the biological activity. The conclusion lays a solid foundation of the clinical application of MDR1 genetic therapy.Keywords: Transfect, human placenta-derived mesenchymal stem cells, multidrug resistance (MDR)1 gene
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