2 research outputs found
Additional file 1: of The potassium channel KCa3.1 constitutes a pharmacological target for astrogliosis associated with ischemia stroke
Figure S1. Cerebral blood flow (CBF) of ischemic brain hemisphere before and during permanent middle cerebral artery occlusion (pMCAO) was monitored by transcranial laser Doppler. The arrow depicted the start of pMCAO. (TIFF 58Â kb
DNA-Conjugated Amphiphilic Aggregation-Induced Emission Probe for Cancer Tissue Imaging and Prognosis Analysis
Detection of an ultralow
concentration of mRNA is important in
the prognosis of gene-related diseases. In this study, a DNA-conjugated
amphiphilic aggregation-induced emission probe (TPE-R-DNA) was synthesized
for cancer tissue imaging and prognosis analysis based on an exonuclease
III-aided target recycling technique. TPE-R-DNA comprise two components:
a hydrophobic component that serves as the “turn-on”
long wavelength fluorescence imaging agent (TPE-R-N<sub>3</sub>);
and a hydrophilic single DNA strand (Alk-DNA) which acts as specific
recognition part for target mRNA. In the absence of target mRNA, TPE-R-DNA
had almost no fluorescence because of its high water solubility. Conversely,
the TPE-R-DNA was digested by exonuclease III (Exo III) in the presence
of MnSOD mRNA to release the hydrophobic fluorogens (TPE-R-AT). Subsequently,
TPE-R-AT formed aggregates, and therefore, fluorescence signal was
distinctly observed. For the first time, the structure of the hydrolysis
product (TPE-R-AT), containing two bases A and T, was proved by the
mass spectrum (MS) and high-performance liquid chromatography (HPLC).
Moreover, the detection limit toward mRNA could be achieved in as
low as 0.6 pM. Furthermore, the fluorescent signal can be used to
confirm the MnSOD mRNA expression level in cancer tissue. The MnSOD
mRNA expression in renal cancer was lower than in renal cancer adjacent
tissue. In particular, the expression level was analyzed to predict
prognosis of cancer patients. Our results demonstrate that a shorter
survival time was evident among patients in lower MnSOD mRNA expression.
Thereby, it indicates great potential for the development of an ultrasensitive
biosensing platform for the application in disease prognosis