Properties and application of polyimide-based composites by blending surface functionalized boron nitride nanoplates

Properties and application of polyimide-based composites by blending surface functionalized boron nitride nanoplate

MiR-326 targets the 3′-untranslated region (UTR) of Bcl-xL but not that of Bak.

<p>(<b>a</b>) Putative miR-326 binding sequences in the 3′-UTR of the Bcl-xL and Bak genes and the mut sequences. (<b>b</b>) Transfection of miR-326 mimic compared to the negative control (miR-NC) significantly inhibited luciferase activity from a reporter vector containing the 3′-UTR of Bcl-xL (**<i>P</i><0.01). However, there was no inhibition of a reporter vector with mutations in the miR-326-binding site (Bcl-xL-mut) (<i>P</i>>0.05). (<b>c</b>) Transfection of miR-326 did not significantly reduce the luciferase activity from reporter vectors containing the 3′-UTR of Bak or Bak-mut (<i>P</i>>0.05). Results represent the means ± SD of luciferase values in 3 independent experiments and are normalized to 1.0 for the miR-NC samples.</p

Association between <i>PCA3</i> promoter STR polymorphisms and prostate carcinoma risk.

a<p>10, 11, 12 and 13 correspond to the total number of <i>TAAA</i> repeat in one allele.</p>b<p>10<i>TAAA</i> group includes the 9<i>TAAA</i> group.</p>c<p>4, 5, 6, 7 and 8 correspond to the number of <i>TAAA</i> repeat.</p

Primers for qRT-PCR.

<p>Primers for qRT-PCR.</p

Bcl-xL siRNA effectively knocks down Bcl-xL mRNA and protein and induces platelet apoptosis.

<p>Leukocyte-depleted apheresis platelets (LDPs) were transfected with siBcl-xL or negative control siRNA (siNC) at a final concentration of 50 nM. LDPs without transfection were used as blank controls (control). Bcl-xL expression and apoptosis were assessed 48 h after transfection. (<b>a</b>) Expression levels of the Bcl-xL mRNA were assessed by qRT-PCR. 5s rRNA was used as an endogenous control. *<i>P</i><0.05. (<b>b,c</b>) Bcl-xL protein levels were assessed by western blotting. β-actin was used as a loading control. *<i>P</i><0.05. (<b>d, e</b>) Depolarization of platelet Δ<i>Ψm</i> was determined by JC-1 fluorochrome staining and flow cytometry. Histograms of platelets with JC-1 staining are shown for mock treated platelets (control) and platelets transfected with siNC or siBcl-xL. Depolarization is characterized as the decrease in the content of JC-1 aggregates, as reflected in the decrease of red (FL2) fluorescence. Dots inside R1 indicate that platelets are out of the main population and are considered to be undergoing Δ<i>Ψm</i> depolarization. *<i>P</i><0.05. (<b>f, g)</b> Annexin V-FITC staining (R2) demonstrated that the annexin V positive cells in LDPs significantly increased as compared to the mock-transfected control and siNC (**<i>P</i><0.01 vs. control, ^##<i>P</i><0.01 vs. siNC). (<b>h</b>) The caspase-3 activity in LDPs was significantly increased as compared to control and negative siRNA (**<i>P</i><0.01 vs. control, ^##<i>P</i><0.01 vs. siNC). Values are expressed as the mean ± SD; n = 3 in each group.</p

MiR-326 promotes platelet apoptosis.

<p>Leukocyte-depleted apheresis platelets (LDPs) were untransfected (control) or transfected with 50 nM of miR-326 mimic or a non-targeting negative control miRNA (miR-NC). LDPs were cultured under standard blood banking conditions and harvested at 24 or 72 h. Flow cytometry was used to analyze the apoptosis status of platelets after transfection. (<b>a, b</b>) Depolarization of <i>ΔΨm</i> was determined by JC-1 fluorochrome assay. <i>*P<</i>0.05. (<b>c</b>) Annexin V positive staining of platelets increased by nearly two-fold after transfection of miR-326 mimic. *P<0.05. (<b>d</b>) Overexpression of miR-326 mimic increased caspase-3 activity in LDP. *P<0.05.</p

Expression of additional Bcl-2 family members after transfection.

<p>Lysates from washed platelets (20 μg) were subjected to western blot analysis for expression of proteins. (<b>a</b>) Western blotting analysis is shown for untransfected platelets (control) and platelets transfected with miR-326 mimic or non-targeting negative control (miR-NC). Leukocyte-depleted apheresis platelets (LDPs) were cultured under standard blood banking conditions and harvested at the indicated times after transfection. Results are representative of 3 independent experiments. (<b>b, c</b>) The effect of miR-326 mimic transfection on Bcl-2 and Bak mRNA expression (<i>P</i>>0.05 vs. siNC).</p

Platelets are not activated by miR-326 expression.

<p>Leukocyte-depleted apheresis platelets (LDPs) were untransfected (control) or treated with 50 nM miR-326 mimic or non-targeting negative control (miR-NC). LDPs were cultured under standard blood banking conditions and harvested at the indicated times. Expression and activity was determined by flow cytometry. (<b>a</b>) Relative number of P-selectin (CD62p)-positive platelets. (<b>b</b>) Relative number of CD63-positive platelets. (<b>c</b>) Relative number of PAC-1-positive platelets. Data were quantified from 3 separate experiments and are shown as mean ± SD.</p

Clinical characteristics.

a<p>Independent-Samples T Test between cases and controls, t = 1.20, P = 0.23.</p

Association between <i>PCA3</i> promoter STR polymorphism and Gleason score in prostate carcinoma.

e<p>Spearman's rank correlation coefficient.</p