Additional file 1 of Tumor-derived exosomal ADAM17 promotes pre-metastatic niche formation by enhancing vascular permeability in colorectal cancer

Supplementary Material

Protein Profiling of Bladder Urothelial Cell Carcinoma

<div><p>This study aimed to detect protein changes that can assist to understand the underlying biology of bladder cancer. The data showed forty five proteins were found to be differentially expressed comparing tumors vs non-tumor tissues, of which EGFR and cdc2p34 were correlated with muscle invasion and histological grade. Ten proteins (ß-catenin, HSP70, autotaxin, Notch4, PSTPIP1, DPYD, ODC, cyclinB1, calretinin and EPO) were able to classify muscle invasive BCa (MIBC) into 2 distinct groups, with group 2 associated with poorer survival. Finally, 3 proteins (P2X7, cdc25B and TFIIH p89) were independent factors for favorable overall survival.</p></div

The Kaplan–Meier and log-rank survival analysis showed that the group with low level expression of P2X7 and high level expression cdc25B and TFIIH p89 was associated with a poorer prognosis (p = 0.032, p = 0.019, and p = 0.005, respectively) and risk score could stratify patients more accurately (p = 0.001).

<p>The Kaplan–Meier and log-rank survival analysis showed that the group with low level expression of P2X7 and high level expression cdc25B and TFIIH p89 was associated with a poorer prognosis (p = 0.032, p = 0.019, and p = 0.005, respectively) and risk score could stratify patients more accurately (p = 0.001).</p

Differentially expressed proteins in different T stages.

<p>a) The hexagonal array displays the changing patterns of the proteins expression from Ta to T4. Each hexagon represents a protein, the color of a hexagon reflects its level of expression in current T stage with respect to corresponding non-tumor tissue. b) The significantly expressed proteins between tumor and non-tumor tissues in different T stages were listed in their own circle which represents a specific stage. The proteins listed in the overlap area were shared by 2 adjacent stages. Proteins in red are upregulated and proteins in green are downregulated.</p

The volcano plot depicts the fold and p-value differences in expression levels of 136 proteins between the tumor and non-tumor tissues.

<p>The horizontal blue line indicates p = 0.05 with points above the line having p < 0.05.</p

Hierarchical clustering analysis of 11 differentially expressed proteins in 31 tumors (T) and 43 non-tumor (N) samples.

<p>The color scale showed the level of expression. Red indicates overexpression, green indicates underexpression, black indicates no change, and gray no expression. The number in each column represents the sample number. Each row represents a protein.</p

The circos plot depicts the relationship between the different signaling pathways and the clinicopathological categories in BCa.

<p>Each signaling pathway and clinicopathological category are assigned with a specific color. The association between the clinicopathological categories and signaling pathways are depicted by the arcs. The area of each colored ribbon depicts the proportion of the signaling pathway contributes to a particular clinicopathological category.</p

Results of uni-variate Cox Proportional HR analyses.

<p>Results of uni-variate Cox Proportional HR analyses.</p

Correlations of protein expression with clinicopathological characteristics.

<p>Correlations of protein expression with clinicopathological characteristics.</p