1 research outputs found
Colorimetric Detection of Carcinogenic Aromatic Amine Using Layer-by-Layer Graphene Oxide/Cytochrome <i>c</i> Composite
Graphene
and its derivatives were found to be efficient modulators
of enzymes in various systems. However, the modulating mechanism was
not well discussed for long time. Inspired by the artificial enzyme-enhancing
property of graphene oxide (GO) toward cytochrome <i>c</i> (cyt. <i>c</i>), we have successfully fabricated a protein/GO
hybrid structure via a layer-by-layer (LbL) strategy. The obtained
LbL assemblies showed great enhancement in peroxidase activity of
cyt. <i>c</i>, as well as excellent stability, resistance
to extreme environment change, and also possibility for recycling
by simple centrifugation without any obvious activity loss. The LbL
cyt. <i>c</i>/GO hybrids were expanded to a colorimetric
sensing system for the detection of carcinogenic aromatic amines.
The probe showed high sensitivity and selectivity for aromatic amines
over various competing soluble aromatic compounds and could be determined
by the naked eye or portable devices. The working mechanism was well
studied through kinetic evaluation, experimental characterization,
and molecular dynamics simulations. This work does not only introduce
a new graphene/protein hybrid material or a rapid and sensitive visualization
of carcinogenic aromatic amines, but also spread the practical application
of biomolecule–graphene interface strategy and further give
a better understanding of the interaction of graphene and protein