864 research outputs found
Mdivi-1, a mitochondrial fission inhibitor, modulates T helper cells and suppresses the development of experimental autoimmune encephalomyelitis.
BACKGROUND: Unrestrained activation of Th1 and Th17 cells is associated with the pathogenesis of multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). While inactivation of dynamin-related protein 1 (Drp1), a GTPase that regulates mitochondrial fission, can reduce EAE severity by protecting myelin from demyelination, its effect on immune responses in EAE has not yet been studied.
METHODS: We investigated the effect of Mdivi-1, a small molecule inhibitor of Drp1, on EAE. Clinical scores, inflammation, demyelination and Drp1 activation in the central nervous system (CNS), and T cell responses in both CNS and periphery were determined.
RESULTS: Mdivi-1 effectively suppressed EAE severity by reducing demyelination and cellular infiltration in the CNS. Mdivi-1 treatment decreased the phosphorylation of Drp1 (ser616) on CD4+ T cells, reduced the numbers of Th1 and Th17 cells, and increased Foxp3+ regulatory T cells in the CNS. Moreover, Mdivi-1 treatment effectively inhibited IFN-γ+, IL-17+, and GM-CSF+ CD4+ T cells, while it induced CD4+ Foxp3+ regulatory T cells in splenocytes by flow cytometry.
CONCLUSIONS: Together, our results demonstrate that Mdivi-1 has therapeutic potential in EAE by modulating the balance between Th1/Th17 and regulatory T cells
Noise and nonlinearities in high-throughput data
High-throughput data analyses are becoming common in biology, communications,
economics and sociology. The vast amounts of data are usually represented in
the form of matrices and can be considered as knowledge networks. Spectra-based
approaches have proved useful in extracting hidden information within such
networks and for estimating missing data, but these methods are based
essentially on linear assumptions. The physical models of matching, when
applicable, often suggest non-linear mechanisms, that may sometimes be
identified as noise. The use of non-linear models in data analysis, however,
may require the introduction of many parameters, which lowers the statistical
weight of the model. According to the quality of data, a simpler linear
analysis may be more convenient than more complex approaches.
In this paper, we show how a simple non-parametric Bayesian model may be used
to explore the role of non-linearities and noise in synthetic and experimental
data sets.Comment: 12 pages, 3 figure
Spin 3/2 Pentaquarks
We investigate the possible existence of the spin 3/2 pentaquark states using
interpolating currents with K-N color-octet structure in the framework of QCD
finite energy sum rule (FESR). We pay special attention to the convergence of
the operator product expansion
Local Magnetic Field Role in Star Formation
We highlight distinct and systematic observational features of magnetic field
morphologies in polarized submm dust continuum. We illustrate this with
specific examples and show statistical trends from a sample of 50 star-forming
regions.Comment: 4 pages, 3 figures; to appear in the EAS Proceedings of the 6th
Zermatt ISM Symposium "Conditions and Impact of Star Formation from Lab to
Space", September 201
Magnetic Fields and Massive Star Formation
Massive stars ( \msun) typically form in parsec-scale molecular clumps
that collapse and fragment, leading to the birth of a cluster of stellar
objects. We investigate the role of magnetic fields in this process through
dust polarization at 870 m obtained with the Submillimeter Array (SMA).
The SMA observations reveal polarization at scales of \lsim 0.1 pc. The
polarization pattern in these objects ranges from ordered hour-glass
configurations to more chaotic distributions. By comparing the SMA data with
the single dish data at parsec scales, we found that magnetic fields at dense
core scales are either aligned within of or perpendicular to the
parsec-scale magnetic fields. This finding indicates that magnetic fields play
an important role during the collapse and fragmentation of massive molecular
clumps and the formation of dense cores. We further compare magnetic fields in
dense cores with the major axis of molecular outflows. Despite a limited number
of outflows, we found that the outflow axis appears to be randomly oriented
with respect to the magnetic field in the core. This result suggests that at
the scale of accretion disks (\lsim 10^3 AU), angular momentum and dynamic
interactions possibly due to close binary or multiple systems dominate over
magnetic fields. With this unprecedentedly large sample massive clumps, we
argue on a statistical basis that magnetic fields play an important role during
the formation of dense cores at spatial scale of 0.01 - 0.1 pc in the context
of massive star and cluster star formation.Comment: Accepted for publication in Astrophysical Journa
Evidence of liquid–liquid transition in glass-forming La50Al35Ni15 melt above liquidus temperature
Liquid–liquid transition, a phase transition of one liquid phase to another with the same composition, provides a key opportunity for investigating the relationship between liquid structures and dynamics. Here we report experimental evidences of a liquid–liquid transition in glass-forming La50Al35Ni15 melt above its liquidus temperature by 27Al nuclear magnetic resonance including the temperature dependence of cage volume fluctuations and atomic diffusion. The observed dependence of the incubation time on the degree of undercooling is consistent with a first-order phase transition. Simulation results indicate that such transition is accompanied by the change of bond-orientational order without noticeable change in density. The temperature dependence of atomic diffusion revealed by simulations is also in agreement with experiments. These observations indicate the need of two-order parameters in describing phase transitions of liquids
New Insights into Traffic Dynamics: A Weighted Probabilistic Cellular Automaton Model
From the macroscopic viewpoint for describing the acceleration behavior of
drivers, this letter presents a weighted probabilistic cellular automaton model
(the WP model, for short) by introducing a kind of random acceleration
probabilistic distribution function. The fundamental diagrams, the
spatio-temporal pattern are analyzed in detail. It is shown that the presented
model leads to the results consistent with the empirical data rather well,
nonlinear velocity-density relationship exists in lower density region, and a
new kind of traffic phenomenon called neo-synchronized flow is resulted.
Furthermore, we give the criterion for distinguishing the high-speed and
low-speed neo-synchronized flows and clarify the mechanism of this kind of
traffic phenomena. In addition, the result that the time evolution of
distribution of headways is displayed as a normal distribution further
validates the reasonability of the neo-synchronized flow. These findings
suggest that the diversity and randomicity of drivers and vehicles has indeed
remarkable effect on traffic dynamics.Comment: 12 pages, 5 figures, submitted to Europhysics Letter
Surgical treatment of congenital biliary duct cyst
<p>Abstract</p> <p>Background</p> <p>It is acknowledged that total cyst excision is a safe and ideal surgical treatment for congenital biliary duct cyst, compared to simple internal drainage. The aim of this study was to determine the optimal operation occasion and the effect of laparoscopy on congenital biliary duct cyst based upon total cyst excision.</p> <p>Methods</p> <p>From January 2002 to January 2011, 217 patients were admitted to Southwest Hospital for congenital biliary duct cyst. To determine the optimal surgery occasion, we divided these subjects into three groups, the infant group (age ≤ 3 years), the immaturity group (3 < age ≤ 18 years), and the maturity group (age > 18 years), and then evaluated the feasibility, risk and long-term outcome after surgery in the three groups. To analyze the effect of laparoscopic technique on congenital biliary duct cyst, we divided the patients into the laparoscopy and the open surgery groups.</p> <p>Results</p> <p>Among the three groups, the morbidity from cholangiolithiasis before surgical treatment had obvious discrepancy (p < 0.05) (lowest in the infant group), and intraoperative blood loss also had apparent diversity (p < 0.05). Furthermore, long-term outcomes (secondary cholangiolithiasis, stoma stenosis and cholangiocarcinoma) showed no significant difference between different groups (p > 0.05).</p> <p>Similarly, no significant discrepancy was observed in the morbidity from postoperative complications or long-term postoperative complications (p > 0.05) between the laparoscopic and the open surgery groups.</p> <p>Conclusions</p> <p>We conclude that total cyst excision should be performed as early as possible. The optimal treatment occasion is the infant period, and laparoscopic resection may be a new safe and feasible minimally invasive surgery for this disease.</p
The comparison of grey-scale ultrasonic and clinical features of hepatoblastoma and hepatocellular carcinoma in children: a retrospective study for ten years
<p>Abstract</p> <p>Background</p> <p>Hepatoblastoma (HBL) and hepatocellular carcinoma (HCC) are respectively the first and the second most common pediatric malignant liver tumors. The purpose of this study was to evaluate the combined use of the ultrasound examination and the assessment of the patients' clinical features for differentiating HBL from HCC in children.</p> <p>Methods</p> <p>Thirty cases of the confirmed HBL and 12 cases of the confirmed HCC in children under the age of 15 years were enrolled into our study. They were divided into the HBL group and the HCC group according to the histological types of the tumors. The ultrasonic features and the clinical manifestations of the two groups were retrospectively analyzed, with an emphasis on the following parameters: onset age, gender (male/female) ratio, positive epatitis-B-surface-antigen (HBV), alpha-fetoprotein increase, and echo features including septa, calcification and liquefaction within the tumors.</p> <p>Results</p> <p>Compared with the children with HCC, the children with HBL had a significantly younger onset age (8.2 years vs. 3.9 years, P < 0.001) and a significantly smaller frequency of positive HBV (66.7% vs. 13.3%, P < 0.001). The septa and liquefaction were more frequently found in HBL than in HCC (25/30, 83.3% vs. 2/12, 16.7%, P < 0.001; 17/30, 56.7% vs. 3/12, 25%, P = 0.02). When a combination of the liquefaction, septa, negative HBV and onset age smaller than 5 years was used in the evaluation, the sensitivity was raised to 90%, the accuracy was raised to 88%, and the negative predictive value was raised to 73%.</p> <p>Conclusion</p> <p>Ultrasonic features combined with clinical manifestations are valuable for differentiating HBL from HCC in children.</p
- …