1 research outputs found
Efficient Coproduction of Mannanase and Cellulase by the Transformation of a Codon-Optimized Endomannanase Gene from Aspergillus niger into Trichoderma reesei
Cellulase
and mannanase are both important enzyme additives in
animal feeds. Expressing the two enzymes simultaneously within one
microbial host could potentially lead to cost reductions in the feeding
of animals. For this purpose, we codon-optimized the Aspergillus niger <i>Man5A</i> gene to
the codon-usage bias of Trichoderma reesei<i>.</i> By comparing the free energies and the local structures
of the nucleotide sequences, one optimized sequence was finally selected
and transformed into the T. reesei pyridine-auxotrophic
strain TU-6. The codon-optimized gene was expressed to a higher level
than the original one. Further expressing the codon-optimized gene
in a mutated T. reesei strain through
fed-batch cultivation resulted in coproduction of cellulase and mannanase
up to 1376 U·mL<sup>–1</sup> and 1204 U·mL<sup>–1</sup>, respectively