Climate warming accelerates temporal scaling of grassland soil microbial biodiversity.

Determining the temporal scaling of biodiversity, typically described as species-time relationships (STRs), in the face of global climate change is a central issue in ecology because it is fundamental to biodiversity preservation and ecosystem management. However, whether and how climate change affects microbial STRs remains unclear, mainly due to the scarcity of long-term experimental data. Here, we examine the STRs and phylogenetic-time relationships (PTRs) of soil bacteria and fungi in a long-term multifactorial global change experiment with warming (+3 °C), half precipitation (-50%), double precipitation (+100%) and clipping (annual plant biomass removal). Soil bacteria and fungi all exhibited strong STRs and PTRs across the 12 experimental conditions. Strikingly, warming accelerated the bacterial and fungal STR and PTR exponents (that is, the w values), yielding significantly (P < 0.001) higher temporal scaling rates. While the STRs and PTRs were significantly shifted by altered precipitation, clipping and their combinations, warming played the predominant role. In addition, comparison with the previous literature revealed that soil bacteria and fungi had considerably higher overall temporal scaling rates (w = 0.39-0.64) than those of plants and animals (w = 0.21-0.38). Our results on warming-enhanced temporal scaling of microbial biodiversity suggest that the strategies of soil biodiversity preservation and ecosystem management may need to be adjusted in a warmer world

Grape-Seed-Derived Procyanidin Attenuates Chemotherapy-Induced Cognitive Impairment by Suppressing MMP-9 Activity and Related Blood–Brain-Barrier Damage

(1) Background: Chemotherapy-induced cognitive impairment (CICI) is often observed in cancer patients and impairs their life quality. Grape-seed-orientated procyanidin has been shown to have anti-inflammatory and neuroprotective effects, yet its effects in preventing CICI have not been investigated. (2) Method: Adult male mice received 2.3 mg/kg cisplatin or saline injections for three cycles consisting of five daily injections followed by 5 days of rest. Procyanidin or saline was administered 1 h prior to cisplatin treatment. Cognitive testing, gelatin zymography, and blood–brain-barrier (BBB) penetration tests were performed after treatment cessation. RAW264.7 cells were treated by stimulated supernatant of SHSY5Y cells. In addition, high-mobility group protein B1 (HMGB1) expression and MMP-9 activity were tested. (3) Results: Repeated cisplatin treatment increased BBB penetration, MMP-9 activity, impaired performance in contextual fear conditioning, and novel object recognition tasks. The knockout of MMP-9 rescues cognitive impairment and cisplatin-induced upregulation of HMGB1 in SHSY5Y cells. HMGB1/TLR4/IP3K/AKT signaling contributes to the increased MMP-9 activity in RAW264.7 cells. Procyanidin treatment attenuates MMP-9 activity, BBB damage, and CICI. (4) Conclusions: The results indicated that MMP-9 activation and BBB disruption is involved in CICI. Procyanidin may effectively alleviate the harmful effects of cisplatin

Rearch on Electromagnetic Scattering from ocean covered by foams

Back scattering of ocean surface is investigated by considering the foams coverage on its surface. Firstly, by adopting modified two-scale mode and the vector radiative transfer theory, zero and first order scattering coefficients are obtained by solving VRT equations. Then, MIE theory is used to get the scattering from foams, and fraction of foams coverage is analyzed by changing parameters of wind speed and temperature difference of ocean-air. Finally, the modified two-scale model and algorithms are verified by comparing its results with measured results, and simulations of calculating back scattering from ocean surface with and without foams are carried out. The conclusion is obtained that wind speed has different influence on back scattering under different incident angles

Development of Flow Cytometric Assay for Detecting Papillary Thyroid Carcinoma Related hsa-miR-146b-5p through Toehold-Mediated Strand Displacement Reaction on Magnetic Beads

In this work, a simple enzyme-free flow cytometric assay (termed as TSDR-based flow cytometric assay) has been developed for the detection of papillary thyroid carcinoma (PTC)-related microRNA (miRNA), hsa-miR-146b-5p with high performance through the toehold-mediated strand displacement reaction (TSDR) on magnetic beads (MBs). The complementary single-stranded DNA (ssDNA) probe of hsa-miR-146b-5p was first immobilized on the surface of MB, which can partly hybridize with the carboxy-fluorescein (FAM)-modified ssDNA, resulting in strong fluorescence emission. In the presence of hsa-miR-146b-5p, the TSDR is trigged, and the FAM-modified ssDNA is released form the MB surface due to the formation of DNA/RNA heteroduplexes on the MB surface. The fluorescence emission change of MBs can be easily read by flow cytometry and is strongly dependent on the concentration of hsa-miR-146b-5p. Under optimal conditions, the TSDR-based flow cytometric assay exhibits good specificity, a wide linear range from 5 to 5000 pM and a relatively low detection limit (LOD, 3σ) of 4.21 pM. Moreover, the practicability of the assay was demonstrated by the analysis of hsa-miR-146b-5p amounts in different PTC cells and clinical PTC tissues

The impact of saturable absorber recovery time on hybrid mode-locked fiber lasers with near zero net dispersion

The hybrid mode-locking scheme holds great potential in obtaining high power ultrashort laser source since it overcomes low damage threshold, self-starting difficulty in single real saturable absorber (SA) or artificial SA mode-locking scheme. Here we numerically studied the effect of SA’s recovery time on the stability of laser output in an all polarization-maintaining (PM) Erbium-doped fiber laser with hybrid mode-locking scheme, operating at the near zero net dispersion. The mapping relationship between fiber fused angles of nonlinear polarization rotation (NPR) section and different mode-locking states is demonstrated. The proportion of stable single pulse operating points increased from 7 % to more than 77 %, as the recovery time of the SA was reduced from 10 ps to 0.1 ps. The results indicate that the laser output states with hybrid mode-locking scheme could be highly affected by the SA recovery time, when the cavity net dispersion is in near zero regime

Analysis of the Spatiotemporal Variation in Land Subsidence on the Beijing Plain, China

Since the 1970s, land subsidence has been rapidly developing on the Beijing Plain, and the systematic study of the evolutionary mechanism of this subsidence is of great significance in the sustainable development of the regional economy. On the basis of Interferometric Synthetic Aperture Radar (InSAR) results, this study employed the Mann–Kendall method for the first time to determine the mutation information of land subsidence on the Beijing Plain from 2004 to 2015. By combining the hydrogeological conditions, “southern water” project, and other data, we attempted to analyse the reasons for land subsidence mutations. First, on the basis of ENVISAT ASAR and RADARSAT-2 data, the land subsidence of the Beijing Plain was determined while using small baseline interferometry (SBAS-InSAR) and Persistent Scatterers Interferometry (PSI). Second, on the basis of the Geographic Information System (GIS) platform, vector data of displacement under different scales were obtained. Through a series of tests, a scale of 960 metres was selected as the research unit and the displacement rate from 2004 to 2015 was obtained. Finally, a trend analysis of land subsidence was carried out on the basis of the Mann–Kendall mutation test. The results showed that single-year mutations were mainly distributed in the middle and lower parts of the Yongding River alluvial fan and the Chaobai River alluvial fan. Among these mutations, the greatest numbers occurred in 2015 and 2005, being 1344 and 915, respectively. The upper and middle alluvial fan of the Chaobai River, the vicinity of the emergency water sources, and the edge of the groundwater funnel have undergone several mutations. Combining hydrogeological data of the study area and the impact of the south-to-north water transfer project, we analysed the causes of these mutations. The experimental results can quantitatively verify the mutation information of land subsidence in conjunction with time series to further elucidate the spatial-temporal variation characteristics of land subsidence in the study area