Cell transcriptomic atlas of the non-human primate Macaca fascicularis.

Studying tissue composition and function in non-human primates (NHPs) is crucial to understand the nature of our own species. Here we present a large-scale cell transcriptomic atlas that encompasses over 1 million cells from 45 tissues of the adult NHP Macaca fascicularis. This dataset provides a vast annotated resource to study a species phylogenetically close to humans. To demonstrate the utility of the atlas, we have reconstructed the cell-cell interaction networks that drive Wnt signalling across the body, mapped the distribution of receptors and co-receptors for viruses causing human infectious diseases, and intersected our data with human genetic disease orthologues to establish potential clinical associations. Our M. fascicularis cell atlas constitutes an essential reference for future studies in humans and NHPs.We thank W. Liu and L. Xu from the Huazhen Laboratory Animal Breeding Centre for helping in the collection of monkey tissues, D. Zhu and H. Li from the Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory) for technical help, G. Guo and H. Sun from Zhejiang University for providing HCL and MCA gene expression data matrices, G. Dong and C. Liu from BGI Research, and X. Zhang, P. Li and C. Qi from the Guangzhou Institutes of Biomedicine and Health for experimental advice or providing reagents. This work was supported by the Shenzhen Basic Research Project for Excellent Young Scholars (RCYX20200714114644191), Shenzhen Key Laboratory of Single-Cell Omics (ZDSYS20190902093613831), Shenzhen Bay Laboratory (SZBL2019062801012) and Guangdong Provincial Key Laboratory of Genome Read and Write (2017B030301011). In addition, L.L. was supported by the National Natural Science Foundation of China (31900466), Y. Hou was supported by the Natural Science Foundation of Guangdong Province (2018A030313379) and M.A.E. was supported by a Changbai Mountain Scholar award (419020201252), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16030502), a Chinese Academy of Sciences–Japan Society for the Promotion of Science joint research project (GJHZ2093), the National Natural Science Foundation of China (92068106, U20A2015) and the Guangdong Basic and Applied Basic Research Foundation (2021B1515120075). M.L. was supported by the National Key Research and Development Program of China (2021YFC2600200).S

Experimental Study on the Anisotropic Characteristics and Engineering Application of Tight Sandstone

The anisotropy of tight sandstone (a type of unconventional gas reservoirs) is a significant factor influencing the characteristics of cracks network under hydrofracturing; thus, it also has a large influence on the final production capacity of the gas reservoirs. To improve the understanding of anisotropy degree and mechanical properties of the tight sandstone of Xujiahe Formation and thus to provide reliable reference for the establishment of hydrofracture model and parameter designing in fracture field, a series of experiments including ultrasonic wave velocity and uniaxial and triaxial compression tests of the tight sandstone samples obtained from Xujiahe Formation with different inclination angles (the angle between sample drilling direction and bedding plane) have been conducted. With the increase of inclination angle, the velocity of the longitudinal wave and elastic modulus both show the tendency of decreasing, whereas the compressive strength shows a “U” shape varying pattern, which is high on sides and low in the middle region. The values of uniaxial compression strength (UCS) are the lowest of sandstone with the inclination angles of 30° and 45°. The fracture patterns are dominant by splitting fracture under uniaxial compression tests. However, shear fracture and dilatancy morphology is the main pattern under triaxial compression test. But the local morphology of the failure surfaces behaves different if the inclination angle is changed. Combining the mechanic theory of transversely isotropic material, the anisotropy parameters of the tight sandstone are analyzed, as well as the influence on the hydrofracturing technology for tight sandstone in the field

Origin of anomalous instability of grid‐forming converters tied to stiff grid

Abstract Grid‐forming (GFM) converter is believed to be highly promising in the future power systems, due to its ability of providing voltage and frequency support. However, some recent studies have shown that the GFM converter may suffer from instability in stiff grids, which seriously hampers its application. In this paper, the mechanism of this anomalous effect is studied by using the small‐signal stability analysis in detail. First, a detailed state‐space model of a single converter grid‐tied system is established from the first principle, and by using the participation factor analysis, the interaction between the terminal voltage loop and the power synchronization loop is identified as the major cause for the system instability. Then relying on a reduced‐order model containing only these two controls and using two classical analytical methods including the Routh criterion analysis and the Phillips– Heffron model of complex torque analysis, the origin of this anomalous instability of GFM converters tied to stiff grid coming from the negative damping provided by the terminal voltage loop is well uncovered and the critical grid strength is well predicted. In addition, these results may provide ideas for subsequent control optimization and stability improvement of GFM converters under various situations

Optimization of the Physical and Mechanical Properties of Grouting Material for Non-Soil-Squeezing PHC Pipe Pile

The physical and mechanical properties of grouting materials greatly affect the friction resistance and the bearing performance of a non-soil-squeezing PHC pipe pile. Orthogonal tests for four factors at five levels were carried out to optimize the proportion of the water–cement mixture by using Portland cement as a raw material and a water-reducing agent, expansion agent and early-strength agent as additives. The following conclusions were obtained: (1) Both the water–cement ratio and the dosage of water-reducing agent are positively correlated with the fluidity of the water–cement mixture and have the greatest influence on the fluidity, followed by the expansion agent and early-strength agent. The saturation point of the water-reducing agent is 1.5%. (2) The strength of the grouting body decreases linearly with the increase of the water–cement ratio, and the dosage of the water-reducing agent has no obvious effect on the strength. As the dosage of expansion agent increases, the strength of the grouting body decreases rapidly. The expansion agent mainly plays a key role in the middle and late stages of the hardening process of the slurry. Early-strength agents have a greater impact on the early strength, but less on the later strength. When the slurry is solidified for 3 h, the early-strength agent has the greatest impact on the strength with an optimal dosage of 5%. (3) The volume of the grouting body has an inverse relationship with the water–cement ratio, and the optimal amount of expansion agent is 12%. The incorporation of an expansion agent makes the volume increase of the grouting body exceed the volume shrinkage ratio caused by the hardening of the grouting body with a curing time of more than 3 days, ensuring a slight increase in the volume of the grouting body. After 3 days, even though the effect of the expansion agent is gradually weakened, it can still ensure that the volume of the grouting body does not shrink. With the increase of the amount of water-reducing agent, the volume of the grouting body gradually decreases. When the amount of water-reducing agent exceeds 1.5%, the volume of the grouting body no longer decreases. (4) The early-strength agent has almost no effect on the volume of the grouting body. When the curing time is 3 h, the water–cement ratio has the greatest influence on the volume of the grouting body, followed by the water-reducing agent, and, finally, the expansion agent. After 3 h, the water–cement ratio still has the greatest influence, and the influence of the expansion agent gradually exceeds that of the water-reducing agent. The water-reducing agent mainly affects the volume of the grouting body in the water separation stage, and the expansion agent mainly plays a role in the middle and late stages of the slurry solidification. After optimized ratio analysis, the fluidity of the water–cement mixture can be improved, the volume shrinkage ratio rate can be lowered and the early strength can be increased

Optimization of the Physical and Mechanical Properties of Grouting Material for Non-Soil-Squeezing PHC Pipe Pile

The physical and mechanical properties of grouting materials greatly affect the friction resistance and the bearing performance of a non-soil-squeezing PHC pipe pile. Orthogonal tests for four factors at five levels were carried out to optimize the proportion of the water–cement mixture by using Portland cement as a raw material and a water-reducing agent, expansion agent and early-strength agent as additives. The following conclusions were obtained: (1) Both the water–cement ratio and the dosage of water-reducing agent are positively correlated with the fluidity of the water–cement mixture and have the greatest influence on the fluidity, followed by the expansion agent and early-strength agent. The saturation point of the water-reducing agent is 1.5%. (2) The strength of the grouting body decreases linearly with the increase of the water–cement ratio, and the dosage of the water-reducing agent has no obvious effect on the strength. As the dosage of expansion agent increases, the strength of the grouting body decreases rapidly. The expansion agent mainly plays a key role in the middle and late stages of the hardening process of the slurry. Early-strength agents have a greater impact on the early strength, but less on the later strength. When the slurry is solidified for 3 h, the early-strength agent has the greatest impact on the strength with an optimal dosage of 5%. (3) The volume of the grouting body has an inverse relationship with the water–cement ratio, and the optimal amount of expansion agent is 12%. The incorporation of an expansion agent makes the volume increase of the grouting body exceed the volume shrinkage ratio caused by the hardening of the grouting body with a curing time of more than 3 days, ensuring a slight increase in the volume of the grouting body. After 3 days, even though the effect of the expansion agent is gradually weakened, it can still ensure that the volume of the grouting body does not shrink. With the increase of the amount of water-reducing agent, the volume of the grouting body gradually decreases. When the amount of water-reducing agent exceeds 1.5%, the volume of the grouting body no longer decreases. (4) The early-strength agent has almost no effect on the volume of the grouting body. When the curing time is 3 h, the water–cement ratio has the greatest influence on the volume of the grouting body, followed by the water-reducing agent, and, finally, the expansion agent. After 3 h, the water–cement ratio still has the greatest influence, and the influence of the expansion agent gradually exceeds that of the water-reducing agent. The water-reducing agent mainly affects the volume of the grouting body in the water separation stage, and the expansion agent mainly plays a role in the middle and late stages of the slurry solidification. After optimized ratio analysis, the fluidity of the water–cement mixture can be improved, the volume shrinkage ratio rate can be lowered and the early strength can be increased

Optimal Placement of Virtual Masses for Structural Damage Identification

Adding virtual masses to a structure is an efficient way to generate a large number of natural frequencies for damage identification. The influence of a virtual mass can be expressed by Virtual Distortion Method (VDM) using the response measured by a sensor at the involved point. The proper placement of the virtual masses can improve the accuracy of damage identification, therefore the problem of their optimal placement is studied in this paper. Firstly, the damage sensitivity matrix of the structure with added virtual masses is built. The Volumetric Maximum Criterion of the sensitivity matrix is established to ensure the mutual independence of measurement points for the optimization of mass placement. Secondly, a method of sensitivity analysis and error analysis is proposed to determine the values of the virtual masses, and then an improved version of the Particle Swarm Optimization (PSO) algorithm is proposed for placement optimization of the virtual masses. Finally, the optimized placement is used to identify the damage of structures. The effectiveness of the proposed method is verified by a numerical simulation of a simply supported beam structure and a truss structure

Experimental Study on Strength of Polypropylene Fiber Reinforced Cemented Silt Soil

To improve the poor characteristics of low strength and high compressibility of weak silty soil, a series of samples with different cement dosage, fiber content, and fiber length was prepared in this experiment, and unconfined compressive strength (UCS) tests, triaxial tests, and scanning electron microscopy (SEM) tests were carried out to explore the influence of polypropylene fiber on the strength of cement-stabilized soil and analyze the curing mechanism of fiber-reinforced cement soil. The test results show that the factors affecting the UCS of the sample from high to low were: cement dosage, fiber content, and fiber length. An orthogonal test found that the optimal ratio of the sample was cement dosage of 18%, fiber content of 0.4%, and fiber length of 3 mm, and the UCS of the sample can reach 1.63 MPa. The triaxial test shows that when the cement dosage is 15% and the fiber length is 9 mm, the incorporation of fiber can significantly improve the toughness and strength of soil. When the cement dosage is 15%, the UCS with 0.4% fiber content is 1.6 times that without fiber. With the increase of fiber content, the peak stress and axial strain of fiber-cured soil are increased, and the cohesion and internal friction angle are also increased. The failure mode and SEM test of fiber-reinforced cement soil show that when the cement dosage is 15% and the fiber length is 9 mm, the addition of fiber can improve the deformation ability of cement soil and slow down the development of cracks. With the increase in fiber content, the number and width of cracks are significantly reduced, and the failure mode changes from brittle failure to ductile failure

An Evaluation Method of Brittleness Characteristics of Shale Based on the Unloading Experiment

Shale reservoir has an initial unloading effect during the natural uplift and erosion process, which causes the shale brittleness to change, affecting the design of the fracturing scheme. To consider this, the axial compression loading and confining pressure unloading experiment of shale is carried out, and then the influence of unloading rate on the mechanical parameters, failure characteristics, and the brittleness of rock are analyzed. What is more, a new evaluation method of brittleness characteristics that take the unloading effect into consideration is proposed. The conclusions are as follows: (1) The unloading rate has a weakening effect on the mechanical parameters, such as the destructive confining pressure and the residual strength of the samples. (2) The failure characteristics of shale specimens are a single shear failure in an oblique section under low unloading rate, and multiple shear zones accompanied with bedding fracture under high unloading rate. (3) The brittleness of shale samples is well verified by the brittleness index B d 1 and B d 2 during the loading path; nevertheless, it has shortage at the unloading path. This paper proposes a new brittleness evaluation method which can consider the influence of the different unloading rates and unloading points. Furthermore, there is a nice characterization between the brittleness damage and this method

Experimental Study on Strength of Polypropylene Fiber Reinforced Cemented Silt Soil

To improve the poor characteristics of low strength and high compressibility of weak silty soil, a series of samples with different cement dosage, fiber content, and fiber length was prepared in this experiment, and unconfined compressive strength (UCS) tests, triaxial tests, and scanning electron microscopy (SEM) tests were carried out to explore the influence of polypropylene fiber on the strength of cement-stabilized soil and analyze the curing mechanism of fiber-reinforced cement soil. The test results show that the factors affecting the UCS of the sample from high to low were: cement dosage, fiber content, and fiber length. An orthogonal test found that the optimal ratio of the sample was cement dosage of 18%, fiber content of 0.4%, and fiber length of 3 mm, and the UCS of the sample can reach 1.63 MPa. The triaxial test shows that when the cement dosage is 15% and the fiber length is 9 mm, the incorporation of fiber can significantly improve the toughness and strength of soil. When the cement dosage is 15%, the UCS with 0.4% fiber content is 1.6 times that without fiber. With the increase of fiber content, the peak stress and axial strain of fiber-cured soil are increased, and the cohesion and internal friction angle are also increased. The failure mode and SEM test of fiber-reinforced cement soil show that when the cement dosage is 15% and the fiber length is 9 mm, the addition of fiber can improve the deformation ability of cement soil and slow down the development of cracks. With the increase in fiber content, the number and width of cracks are significantly reduced, and the failure mode changes from brittle failure to ductile failure

Simplified Seismic Loss Estimation of RC Frame using Component-performance-based Methodology

A framework of simplified probabilistic seismic loss estimation of RC frame using component- performance -based methodology was presented in this paper. Firstly, component- performance -based methodology was introduced, and component performance level was measured using elasto-plastic rotation angle of structural component. Then structural performance level was determined by the statistics of damaged components distribution. Additionally, the relationship between the structural performance level and loss ratio of RC frame was established. Last, expected seismic loss ratio of 3-storey RC frame was studied in detail. This study reveals that the simplified probabilistic framework proposed herein could be used in RC frame