STUDY ON LOAD MEASURING METHOD AND TEST OF THE PIN-SHAFT IN LOADER WORKING DEVICE

Based on the mechanical force model of the bucket on the working device of loader,the problem of the dynamic loading test was analyzed. The testing method considering pin-shaft lateral dynamic force was put forward,and the 3D forces in horizontal,vertical and lateral direction of the loader working device were accurately measured. Without changing the loading characteristics of the working device,the pin-shaft dynamic force test system was established with the pin-shaft sensor design and the bucket structure modification. Primary soil containing granular iron ore was chosen as the shovel loading operation case,and the dynamic forces in three directions of the pin-shaft linking the arm and the bucket were acquired through the shovel loading test. The results show that: the dynamic testing method and the system could accurately obtain the dynamic loads of three directions of the pin-shaft; the dynamic loads of the three directions measured by the pin-shaft sensors are closely related to the loading process; the dynamic loads of the lateral force of the loader working device with the change of loading process were obtained firstly. It has important guiding roles for the study on the mechanical characteristics of the loader working device,the acquisition of the load spectrum and the prediction of fatigue life

Study on Stress Analysis and Welding Deformation of the Loader Boom

This paper takes the 5T loader boom as the research object. Firstly, the static analysis of the boom loader is carried out under the typical working conditions. The weak part of the welding seam is found, and then the welding deformation of the boom is analyzed for the weak welding part. Further, the deformation trends of the welding arm are studied. It is found that the weak part of the welding seam is at the welding arc of the boom plate and the beam. Besides, the deformation of the arm plate is about 30mm inward in the welding process due to the welding stress, and the residual stress of the boom. This work can provide theoretical and data support for the processing and deformation correction of the boom

Study on Stress Analysis and Welding Deformation of the Loader Boom

This paper takes the 5T loader boom as the research object. Firstly, the static analysis of the boom loader is carried out under the typical working conditions. The weak part of the welding seam is found, and then the welding deformation of the boom is analyzed for the weak welding part. Further, the deformation trends of the welding arm are studied. It is found that the weak part of the welding seam is at the welding arc of the boom plate and the beam. Besides, the deformation of the arm plate is about 30mm inward in the welding process due to the welding stress, and the residual stress of the boom. This work can provide theoretical and data support for the processing and deformation correction of the boom

An integrated multi-source energy harvester based on vibration and magnetic field energy

In this paper, an integrated multi-source energy harvester (IMSEH) employing a special shaped cantilever beam and a piezoelectric transducer to convert vibration and magnetic field energy into electrical energy is presented. The electric output performance of the proposed IMSEH has been investigated. Compared to a traditional multi-source energy harvester (MSEH) or single source energy harvester (SSEH), the proposed IMSEH can simultaneously harvest vibration and magnetic field energy with an integrated structure and the electric output is greatly improved. When other conditions keep identical, the IMSEH can obtain high voltage of 12.8V. Remarkably, the proposed IMSEHs have great potential for its application in wireless sensor network

A Blueprint of Microstructures and Stage-Specific Transcriptome Dynamics of Cuticle Formation in Bombyx mori

Insect cuticle is critical for the environmental adaptability and insecticide resistance of insects. However, there is no clear understanding of the structure and protein components of the cuticle during each developmental stage of holometabolous insects, and knowledge about the protein components within each layer is vague. We conducted serial sectioning, cuticular structure analysis, and transcriptome sequencing of the larval, pupal, and adult cuticles of Bombyx mori. The deposition processes of epicuticle, exocuticle, and endocuticle during larval, pupal, and adult cuticle formation were similar. Transcriptome analysis showed that these cuticle formations share 74% of the expressed cuticular protein (CP) genes and 20 other structural protein genes, such as larval serum protein and prisilkin. There are seven, six, and eleven stage-specific expressed CP genes in larval, pupal, and adult cuticles, respectively. The types and levels of CP genes may be the key determinants of the properties of each cuticular layer. For example, the CPs of the RR-2 protein family with high contents of histidine (His) are more essential for the exocuticle. Functional analysis suggested that BmorCPAP1-H is involved in cuticle formation. This study not only offers an in-depth understanding of cuticle morphology and protein components but also facilitates the elucidation of molecular mechanisms underlying cuticle formation in future studies

Table_2_Alternative splicing signature of alveolar type II epithelial cells of Tibetan pigs under hypoxia-induced.XLS

Alternative splicing (AS) allows the generation of multiple transcript variants from a single gene and affects biological processes by generating protein diversity in organisms. In total, 41,642 AS events corresponding to 9,924 genes were identified, and SE is the most abundant alternatively spliced type. The analysis of functional categories demonstrates that alternatively spliced differentially expressed genes (DEGs) were enriched in the MAPK signaling pathway and hypoxia-inducible factor 1 (HIF-1) signaling pathway. Proteoglycans in cancer between the normoxic (21% O2, TN and LN) and hypoxic (2% O2, TL and LL) groups, such as SLC2A1, HK1, HK2, ENO3, and PFKFB3, have the potential to rapidly proliferate alveolar type II epithelial (ATII) cells by increasing the intracellular levels of glucose and quickly divert to anabolic pathways by glycolysis intermediates under hypoxia. ACADL, EHHADH, and CPT1A undergo one or two AS types with different frequencies in ATII cells between TN and TL groups (excluding alternatively spliced DEGs shared between normoxic and hypoxic groups), and a constant supply of lipids might be obtained either from the circulation or de novo synthesis for better growth of ATII cells under hypoxia condition. MCM7 and MCM3 undergo different AS types between LN and LL groups (excluding alternatively spliced DEGs shared between normoxic and hypoxic groups), which may bind to the amino-terminal PER-SIM-ARNT domain and the carboxyl terminus of HIF-1α to maintain their stability. Overall, AS and expression levels of candidate mRNAs between Tibetan pigs and Landrace pigs revealed by RNA-seq suggest their potential involvement in the ATII cells grown under hypoxia conditions.</p

Data_Sheet_2_Alternative splicing signature of alveolar type II epithelial cells of Tibetan pigs under hypoxia-induced.doc

Alternative splicing (AS) allows the generation of multiple transcript variants from a single gene and affects biological processes by generating protein diversity in organisms. In total, 41,642 AS events corresponding to 9,924 genes were identified, and SE is the most abundant alternatively spliced type. The analysis of functional categories demonstrates that alternatively spliced differentially expressed genes (DEGs) were enriched in the MAPK signaling pathway and hypoxia-inducible factor 1 (HIF-1) signaling pathway. Proteoglycans in cancer between the normoxic (21% O2, TN and LN) and hypoxic (2% O2, TL and LL) groups, such as SLC2A1, HK1, HK2, ENO3, and PFKFB3, have the potential to rapidly proliferate alveolar type II epithelial (ATII) cells by increasing the intracellular levels of glucose and quickly divert to anabolic pathways by glycolysis intermediates under hypoxia. ACADL, EHHADH, and CPT1A undergo one or two AS types with different frequencies in ATII cells between TN and TL groups (excluding alternatively spliced DEGs shared between normoxic and hypoxic groups), and a constant supply of lipids might be obtained either from the circulation or de novo synthesis for better growth of ATII cells under hypoxia condition. MCM7 and MCM3 undergo different AS types between LN and LL groups (excluding alternatively spliced DEGs shared between normoxic and hypoxic groups), which may bind to the amino-terminal PER-SIM-ARNT domain and the carboxyl terminus of HIF-1α to maintain their stability. Overall, AS and expression levels of candidate mRNAs between Tibetan pigs and Landrace pigs revealed by RNA-seq suggest their potential involvement in the ATII cells grown under hypoxia conditions.</p