Metagenomic Next-Generation Sequencing in the Diagnosis of HHV-1 Reactivation in a Critically Ill COVID-19 Patient: A Case Report

Background: Secondary infections pose tremendous challenges in Coronavirus disease 2019 (COVID-19) treatment and are associated with higher mortality rates. Clinicians face of the challenge of diagnosing viral infections because of low sensitivity of available laboratory tests. Case Presentation: A 66-year-old woman initially manifested fever and shortness of breath. She was diagnosed as critically ill with COVID-19 using quantitative reverse transcription PCR (RT-qPCR) and treated with antiviral therapy, ventilator and extracorporeal membrane oxygenation (ECMO). However, after the condition was relatively stabled for a few days, the patient deteriorated with fever, frequent cough, increased airway secretions, and increased exudative lesions in the lower right lung on chest X-rays, showing the possibility of a newly acquired infection, though sputum bacterial and fungal cultures and smears showed negative results. Using metagenomic next-generation sequencing (mNGS), we identified a reactivation of latent human herpes virus type 1 (HHV-1) in the respiratory tract, blood and gastrointestinal tract, resulting in a worsened clinical course in a critically ill COVID-19 patient on ECMO. Anti-HHV-1 therapy guided by these sequencing results effectively decreased HHV-1 levels, and improved the patient\u27s clinical condition. After 49 days on ECMO and 67 days on the ventilator, the 66-year-old patient recovered and was discharged. Conclusions: This case report demonstrates the potential value of mNGS for evidence-based treatment, and suggests that potential reactivation of latent viruses should be considered in critically ill COVID-19 patients

The Development of Quality Indicator System for the Home Care Services for the Disabled Elderly in Beijing

Background With the deepening of aging in China, there is an increasing demand for home care services for disabled elderly individuals. The evaluation of service quality provides an assurance for the implementation of high-quality services. Objective To develop a quality indicator system for the delivery of home care services to the disabled elderly population in Beijing based on the Delphi method, so as to offer objective criteria for evaluating the quality of home care services provided by community health service institutions. Methods Based on literature research and qualitative study, a preliminary pool of indicator system entries for the indicator system was formed, and an expert correspondence form was designed from March to May 2021. A total of 21 community nursing, general practice, and management research personnel experts with rich experience in the field of home medical services and elderly comprehensive assessment in Beijing were selected, and an expert correspondence form was sent to them by email from June to October 2021 to conduct three rounds of Delphi expert correspondence. The experts' personal information and authority were recorded and analyzed, to the establish a quality indicator system for home care services for disabled elderly individuals in Beijing. Results All three rounds of expert correspondence form were returned and valid, with a 100.00% positive coefficient of experts from the 21 selected experts who had a mean expert authority score of 0.927 (ranging from 0.700 to 1.000), indicating an acceptable level of expertise. The first, second, and third rounds of expert correspondence yielded importance and feasibility coordination coefficients of 0.170 and 0.140 (P<0.001), 0.147 and 0.175 (P<0.001), 0.231 and 0.208 (P<0.001), respectively, indicating a high level of consensus among the experts and the reliability of the correspondence results. The resulting quality indicator system for home care services for disabled elderly individuals included three primary indicators of service conditions, service processes, and service outcomes, as well as nine secondary indicators and 34 tertiary indicators. Conclusion The indicator system constructed in this study is appropriate for evaluating the implementation quality of single visit medical service, and providing a reference for community health service institutions to formulate regulations and work programs

Comparison of Phytochemical Differences of the Pulp of Different Peach [<i>Prunus persica</i> (L.) Batsch] Cultivars with Alpha-Glucosidase Inhibitory Activity Variations in China Using UPLC-Q-TOF/MS

In order to fully understand the variation of the fruit alpha-glucosidase inhibitory activity-related phytochemical basis in the Chinese peach [Prunus persica (L.) Batsch], mature fruit from 33 cultivars was used for the investigation of fruit phenolic phytochemical attributes, including total phenolics, flavonoids, anthocyanins, and procyanidins, as well as the alpha-glucosidase inhibitory activity in vitro. Alpha-glucosidase inhibitory activity varied significantly among tested peach cultivars and was strongly correlated with total phenolics, total procyanidins, and total flavonoids. Untargeted UPLC-Q-TOF/MS-based metabolomics were used to comprehensively discriminate between peaches with different inhibitory activity on alpha-glucosidase. Principal component analysis (PCA) and orthogonal partial least squares discrimination analysis (OPLS-DA) were used for this process. Twenty-three differential compounds were identified between peach cultivars with high and low alpha-glucosidase inhibitory activity, and nine, including procyanidin C1, procyanidin trimer isomer 1, procyanidin trimer isomer 2, procyanidin B1, procyanidin dimer, epicatechin-epicatechin-epicatechin, phloridzin, kaempferol 3-(2&#8217;&#8217;,6&#8217;&#8217;-di-(E)-p-coumarylglucoside), and luteolin 3&#8217;-methyl ether 7-malonylglucoside, were identified as marker compounds responsible for the discrimination. Overall, variations in metabolites in peach pulp reflect the diversity in peach germplasm, and these nine compounds are good candidate markers for future genetic breeding of peach fruit with high alpha-glucosidase inhibitory activity

Composition of dissolved organic matter controls interactions with La and Al ions : Implications for phosphorus immobilization in eutrophic lakes

Applications of aluminium (Al) salt or lanthanum (La) modified bentonite (LMB) have become popular methodologies for immobilizing phosphorus (P) in eutrophic lakes. The presence of humic substances, has been shown to inhibit this form of treatment due to the complexation with La/Al. However, the effects of other dissolved organic matter (DOM), especially that derived from phytoplankton (the dominant source in eutrophic lakes) are unknown. In this study, the interaction with La/Al of Suwannee River Standard Humic Acid Standard II (SRHA) and algae-derived DOM (ADOM) were investigated and compared. Differed to SRHA which was dominated by polyphenol-like component (76.8%, C1-SRHA), majority in ADOM were protein-like substance, including 41.9% tryptophan-like component (C2-ADOM) and 21.0% tyrosine-like component (C3-ADOM). Two reactions of complexation and coprecipitation were observed between SRHA/ADOM and La/Al. Complexation dominated at low metal inputs less than 10 μM and coprecipitation was the main reaction at higher metal inputs. For ADOM, the tryptophan-like component (C2-ADOM) was the important component to react with metal. The reaction rate for C2-ADOM with La were about two-third of that for C1-SRHA, indicating that the influence of C2-ADOM was significant during the P immobilization by La/Al-based treatment in eutrophic lakes. The P removal data in the presence of ADOM confirmed the significant inhibition of ADOM. In addition, based on the composition of coprecipitates and relatively biodegradable character of tryptophan-like substances (C2-ADOM), the coprecipitation of ADOM was assumed to reduce the stability of precipitated P in eutrophic lakes. The release of P from the potential biodegradation of the coprecipitates and thus the possible decline of the performance of P immobilization by La/Al-based treatments is an important work in the future.</p

Local-scale soil nematode diversity in a subtropical forest depends on the phylogenetic and functional diversity of neighbor trees

Purpose Understanding the impact of neighbor tree diversity on soil biodiversity at the individual tree scale and clarifying which facets of neighbor tree diversity have a decisive impact on soil biodiversity. Methods We collected and identified soil nematodes underneath 256 individual trees of 16 species at four species-richness levels (1, 2, 4, 8 species) in a large tree diversity experiment in southeast China. We analyzed how the taxonomic, phylogenetic, and functional diversity of the nematode community were influenced by neighbor tree diversity. Results Our analyses showed that nematode alpha diversity at the individual tree scale decreased with increasing neighbor tree richness while beta diversity increased at taxonomic, phylogenetic, and functional levels. Nematode alpha diversity at the plot scale increased with increasing neighbor tree richness. Secondly, we found that reducing redundant species and increasing distinct species led to the differentiation of nematode communities under different individual trees in high-richness plots. Finally, our data revealed that the functional diversity of the neighbor tree community had the largest effects on the diversity of the local nematode community. Conclusion Our study emphasizes that there is a significant influence from neighboring trees, mainly the functional traits of the trees, even though they are spaced at distances multitudes greater than the distance over which soil nematodes can move. Hence it is important to consider these broader spatial aspects when examining the plant and soil biotic interactions

Comparison of Waterlogging Tolerance of Three Peach Rootstock Seedlings Based on Physiological, Anatomical and Ultra-Structural Changes

Peach (Prunus persica (L.) Batsch) is a typical shallow-rooted fruit plant with a high respiratory intensity and oxygen demand, which makes it highly susceptible to oxygen-deficient soil conditions resulting from waterlogging. Rootstock waterlogging resistance is essential to the performance of cultivated peaches under waterlogging stress. In comparison to Prunus persica var. persica (&lsquo;Maotao&rsquo;, M) and Prunus davidiana (Carr.) C. de Vos (&lsquo;Shantao&rsquo;, S), Prunus persica f. Hossu (&lsquo;Hossu&rsquo;, H) exhibited superior leaf photosynthetic electron transfer efficiency, a higher rate of mycorrhizal fungi infection in both fine roots and mesophyll palisade cells, as well as earlier air cavity formation in both leaf midvein and fine roots under waterlogging stress. Furthermore, under non-waterlogging conditions, Hossu had greater leaf superoxide dismutase (SOD) activity, higher proline content, and a greater content of starch granules in the pith and xylem ray cells of stems and roots than rootstocks M and S. As a result, Hossu&rsquo;s tolerance to waterlogging may be due to its higher photosynthetic efficiency, improved tissue oxygen permeability, higher energy metabolism, and increased intracellular mycorrhizal fungus infection rates in both root parenchyma cells and mesophyll palisade cells