An immunohistochemistry analysis of hypoxia inducible factor-2α (HIF-2α).

<p>Control group (a), HIF-2α protein levels on the 2^nd week (f vs a, b, c) (P<0.01), and the 2^nd to 4^th week (P<0.05). Original magnification, ×200. Scale bars: 20 µm.</p

The immunohistochemistry analysis of epithelial cell kinase (EphA2).

<p>Control group (a), EphA2 protein levels from the 1st day to the 1st week (b, c, d, e) (P<0.01), and from the 2nd week to the 4th week (a vs h P<0.05). Original magnification, ×200. Scale bars: 20 µm.</p

MVD analyses.

<p>Control group (a), micro-vessel density (MVD) from the 1^st day to the 1^st week (b, c, d, e) (P<0.01), and from the 2nd week to the 4th week (g vs a, P<0.05). Original magnification, ×200. Scale bars:20 µm.</p

An immunohistochemistry analysis of vascular endothelial growth factor A (VEGF-A).

<p>Control group (a), HIFU treatment from the 1st day to the 1st week (b, c, d, e) (P<0.01), and from the 2st week to the 4st week (a vs h, P<0.05). Original magnification, ×200. Scale bars: 20 µm.</p

Hypoxia inducible factor-1, 2α, VEGFA, and EphA2 mRNA expression measured by Q-PCR.

<p>HIF-1α mRNA expression at the 3^rd day (P<0.01), compared with the control, and other groups of the time points (P<0.05). HIF-2α mRNA at the 2^nd week (P<0.01) and levels of VEGFA and EphA2 mRNA at various time points from the 1st day to the 1st week, P<0.05, and from the 3 w to 4 w, P<0.05, compared with control group, and the 2nd week vs the control group, P>0.05.</p

The VEGFA and EphA2 protein expression after HIFU treatment from 1 d to 1 w, compared with the control group (P<0.01) and from the 3 w to 4 w compared to the control group, respectively (P<0.05).

<p>(2nd week vs control group, P>0.05).</p

The HIF-2α protein levels on the 2^nd week, compared with the control group (P<0.01), from the 2^nd to 4^th week (P<0.05, compared with the control group).

<p>The HIF-2α protein levels on the 2^nd week, compared with the control group (P<0.01), from the 2^nd to 4^th week (P<0.05, compared with the control group).</p

The picture shows residual hepatic tumor tissue after high intensity focused ultrasound (HIFU) treatment.

<p>(<b>a</b>) The residual hepatic tumor tissue was obtained from the subcutaneously transplanted tumor. (<b>b</b>) The cut surface of the residual tumor tissue. (<b>c</b>) Residual tumor tissue with hematoxylin and eosin staining, revealing necrotic cells (<b>d</b>), inflammatory cells (<b>e</b>), and normal hepatoma cells (<b>f</b>). Original magnification, ×200. Scale bars: 20 µm.</p

HIF-1α protein expression on the 3^rd day, compared with the control group and other groups of the time points (P<0.01).

<p>(1^st day vs the 1^st week, P>0.05, from the 2 w to 4 w group, compared with each other, P>0.05).</p