Batch cultivation of <i>R. eutropha</i> for PHBV accumulation under the condition of stable pH and DO, which was automatically kept at 7.0 and 30%, respectively.

<p>1.2 L (pH 7.0) of media was in 2 L fermenter with 5% (v/v) inoculum at 30°C and aeration.</p

DSC thermograms of PHB (a), PHBV₁ (b) and PHBV₂ (c) samples.

<p>DSC thermograms of PHB (a), PHBV₁ (b) and PHBV₂ (c) samples.</p

Effect of different glucose concentrations on DCW, PHBV and HV production.

<p>Sharing a common lowercase are not significantly different in the HV content and the same capital are not significantly different in the concentration of DCW and PHBV (<i>P</i><0.05). 50 mL (pH 7.0) of media was in 250 mL flask with 5% (v/v) inoculum under shaking conditions at 200 rpm for 72 h at 30°C.</p

TG-DTG curve of PHB (a), PHBV1 (b) and PHBV2 (c) samples.

<p>TG-DTG curve of PHB (a), PHBV1 (b) and PHBV2 (c) samples.</p

Batch cultivation of <i>R. eutropha</i> for PHBV accumulation under the condition of stable pH, which was automatically fed to keep the pH of the culture at 7.0. 1.2 L (pH 7.0) of media was in 2 L fermenter with 5% (v/v) inoculum at 30°C and aeration/agitation (500 rpm and 2 vvm air volume/culture volume/min).

<p>Batch cultivation of <i>R. eutropha</i> for PHBV accumulation under the condition of stable pH, which was automatically fed to keep the pH of the culture at 7.0. 1.2 L (pH 7.0) of media was in 2 L fermenter with 5% (v/v) inoculum at 30°C and aeration/agitation (500 rpm and 2 vvm air volume/culture volume/min).</p

Thermal properties of PHBV samples with different fractions of HV.

<p>Xc: crystallinity, Tm: melting point, ▵H_f: melting enthalpy, T_eoi: initial thermal decomposition temperature, T_em: onset temperature, T_eof: finished thermal decomposition temperature, Wt-loss: thermal weight loss.</p

Effects of initial pH on DCW, PHBV and HV production.

<p>Sharing a common lowercase are not significantly different in the HV content and the same capital are not significantly different in the concentration of DCW and PHBV(<i>P</i><0.05). 50 mL of media was in 250 mL flask with 5% (v/v) inoculum under shaking conditions at 200 rpm for 72 h at 30°C.</p

Effect of different LA concentrations on DCW, PHBV and HV production.

<p>Sharing a common lowercase are not significantly different in the HV content and the same capital are not significantly different in the concentration of DCW and PHBV (<i>P</i><0.05). 50 mL (pH 7.0) of media was in 250 mL flask with 5% (v/v) inoculum under shaking conditions at 200 rpm for 72 h at 30°C.</p

Effects of NH₄Cl (a) and casein peptone (b) concentration on DCW, PHBV and HV production.

<p>Sharing a common lowercase are not significantly different in the HV content and the same capital are not significantly different in the concentration of DCW and PHBV (<i>P</i><0.05). 50 mL (pH 7.0) of media was in 250 mL flask with 5% (v/v) inoculum under shaking conditions at 200 rpm for 72 h at 30°C.</p