Clinical report about the effectiveness of andriol, tamoxifen, vitamin A and lecithin on low acrosome

BackgroundAcrosin is the most important proteolytic enzyme in fertilization, acrosome dysfunction affects fertilization. Recently, our group found that the treatment of andriol, tamoxifen, vitamin A and lecithin helped to improve acrosome function.AimsTo assess the effectiveness of andriol, tamoxifen, vitamin A and lecithin on low acrosome.MethodsSeventy-eight infertility males in my hospital were enrolled. Kenedy test was used to test acrosome function. Andriol, tamoxifen, vitamin A and lecithin were used for one to four months.ResultsSperm concentration (t=5.05, P=0.000) raised while seminal volume (t=1.93, P=0.057), normal morphology (t=0.24, P=0.811) and progressive sperm (t=2.14, P=0.036) did not show statistical difference after treatment. Acrosin was (37.62±11.07)μIU/106 sperm before treatment and raised to (52.68±15.68)μIU/106 sperm after treatment and showed statistical difference (t=8.18, P=0.00). In all the 78 males, the acrosin of seven men (11 per cent) bellowed after treatment while the acrosin of 71 males (91 per cent) raised and ten couples (13 per cent) got pregnant.ConclusionThe combined treatment of andriol, tamoxifen, vitamin A and lecithin were effective on low acrosin

Effectiveness of two kind of medicine on non-obstructive azoospermia

ObjectiveTo assess the effectiveness of medicine on non-obstructive azoospermia (NOA). Cases32 male patients were enrolled from March 2012 to March 2016, with age range of 23–41 years old and infertility time of 1–3 years.Methods According to “WHO Human Semen Detection and Processing Experiment Manual";;;;;; fifth edition, semen routine was based on manual detection and serum hormones based on chemiluminescence detection, semen volume, sperm density, forward moving sperm ratio. If sperm was detected in semen for more than two consecutive times, the treatment was considered effective and was included into the statistical analysis. The detection parameters took the first test data and the last test data. Treatment Andriol and tamoxifen were used for idiopathic NOA while aescuven forte and aspirin used for NOA with varicocele (including two varicocelectomy). The time was four months.ResultsOf the 32 patients, 13 underwent testicular biopsy, pathological results showed six patients (46 per cent) with hypospermatogenesis, five patients (39 per cent) with maturation arrest, and two patients (15 per cent) with sertoli cell syndrome. Nine patients (28 per cent) had varicocele; two patients (6 per cent) underwent surgical treatment. After treatment, sperm was detected in 20 patients (63 per cent), seven of them (22 per cent) had detectable sperm in semen after one month of treatment; five (19 per cent) had sperm after two months of treatment; eight (25 per cent) had sperm after three months of treatment. Among the 23 patients with idiopathic azoospermia, 13 patients had detectable sperm in semen after treatment (56 per cent). The level of hormone gradually increased in 15 patients, rapidly increased in four patients, but four patients had no obvious change of hormone level. Among the nine patients with varicocele, three persons (including one patient undergoing surgery) had normal sperm density after 3–4 months of treatment, continuing the treatment for another 2–3 months, wife of the patients gained pregnancies. After treatment, seven patients had gradually decreasing serum level of FSH, LH and T while two patients had increased serum level of hormones. Excluding the three pregnancy patients, it was showed that the sperm density increased (t=-3.342, p=0.004) after treatment, so did the forward moving sperm ratio (t=-4.037, p=0.001), but semen volume had no significant changes (t=-1.698, p=0.109). ConclusionThe treatment of varococele is important for the NOA with varicocele while andriol and tamoxifen is other method for the treatment of idiopathic NOA

Porcine epidemic diarrhea virus causes diarrhea by activating EGFR to regulates NHE3 activity and mobility on plasma membrane

As part of the genus Enteropathogenic Coronaviruses, Porcine Epidemic Diarrhea Virus (PEDV) is an important cause of early diarrhea and death in piglets, and one of the most difficult swine diseases to prevent and control in the pig industry. Previously, we found that PEDV can block Na+ absorption and induce diarrhea in piglets by inhibiting the activity of the sodium-hydrogen ion transporter NHE3 in pig intestinal epithelial cells, but the mechanism needs to be further explored. The epidermal growth factor receptor (EGFR) has been proved to be one of the co-receptors involved in many viral infections and a key protein involved in the regulation of NHE3 activity in response to various pathological stimuli. Based on this, our study used porcine intestinal epithelial cells (IPEC-J2) as an infection model to investigate the role of EGFR in regulating NHE3 activity after PEDV infection. The results showed that EGFR mediated viral invasion by interacting with PEDV S1, and activated EGFR regulated the downstream EGFR/ERK signaling pathway, resulting in decreased expression of NHE3 and reduced NHE3 mobility at the plasma membrane, which ultimately led to decreased NHE3 activity. The low level of NHE3 expression in intestinal epithelial cells may be a key factor leading to PEDV-induced diarrhea in newborn piglets. This study reveals the importance of EGFR in the regulation of NHE3 activity by PEDV and provides new targets and clues for the prevention and treatment of PEDV-induced diarrhea in piglets

Low-latency Data Computation of Inland Waterway USVs for RIS-Assisted UAV MEC Network

Unmanned Surface Vehicles (USVs) in inland waterways have drawn increasing attention for their excellent capability to serve maritime time-consuming missions such as autonomous navigation and intelligent monitoring. However, USVs struggle to accomplish emerging computation-intensive tasks (e.g., sensor, telemetry, etc) timely due to the limited on-board resources. This paper proposes a novel reconfigurable intelligent surface (RIS)-assisted unmanned aerial vehicle (UAV) multi-access edge computing (MEC) network architecture to support low-latency USVs data computation with time window. Aiming to enhance USVs task processing efficiency, the minimization of USVs task processing time is formulated by jointly considering UAVs flight route selection, USVs execution mode selection, UAVs hovering coordinates and RIS phase shift vector. A heuristic solution is proposed to tackle the formulated challenging problem iteratively. The original problem is decoupled into three subproblems: an enhanced deferred acceptance algorithm is proposed to solve UAVs flight route selection subproblem; an enhanced Lagrangian relaxation method is proposed to solve USVs execution mode selection subproblem; a joint alternating direction method of multipliers (ADMM)-successive convex approximation (SCA)-based algorithm is proposed to solve UAVs hovering coordinates subproblem. Experiment results demonstrate that the proposed solution can decrease task processing time by approximately 54% compared with numerous selected advanced algorithms. Moreover, the performance of the proposed solution under typical UAVs caching capability and the number of UAVs has been investigated

Mechanism of Lactiplantibacillus plantarum regulating Ca2+ affecting the replication of PEDV in small intestinal epithelial cells

Porcine epidemic diarrhea virus (PEDV) mainly invades the small intestine and promotes an inflammatory response, eventually leading to severe diarrhea, vomiting, dehydration, and even death of piglets, which seriously threatens the economic development of pig farming. In recent years, researchers have found that probiotics can improve the intestinal microenvironment and reduce diarrhea. At the same time, certain probiotics have been shown to have antiviral effects; however, their mechanisms are different. Herein, we aimed to investigate the inhibitory effect of Lactiplantibacillus plantarum supernatant (LP-1S) on PEDV and its mechanism. We used IPEC-J2 cells as a model to assess the inhibitory effect of LP-1S on PEDV and to further investigate the relationship between LP-1S, Ca2+, and PEDV. The results showed that a divalent cation chelating agent (EGTA) and calcium channel inhibitors (Bepridil hydrochloride and BAPTA-acetoxymethylate) could inhibit PEDV proliferation while effectively reducing the intracellular Ca2+ concentration. Furthermore, LP-1S could reduce PEDV-induced loss of calcium channel proteins (TRPV6 and PMCA1b), alleviate intracellular Ca2+ accumulation caused by PEDV infection, and promote the balance of intra- and extracellular Ca2+ concentrations, thereby inhibiting PEDV proliferation. In summary, we found that LP-1S has potential therapeutic value against PEDV, which is realized by modulating Ca2+. This provides a potential new drug to treat PEDV infection

EGFR as a Negative Regulatory Protein Adjusts the Activity and Mobility of NHE3 in the Cell Membrane of IPEC-J2 Cells With TGEV Infection

Transmissible gastroenteritis (TGE) has caused devastating economic losses to the swine industry worldwide, despite extensive research focusing on the pathogenesis of virus infection. The molecular pathogenic mechanism of TGEV-induced diarrhea in piglets is unknown. Intestinal diarrhea is closely related to the function of the Na+/H+ exchanger protein NHE3 in the brush border membrane of small intestine epithelial cells. The epidermal growth factor receptor (EGFR) may act to regulate NHE3 expression. In addition, EGFR may promote viral invasion of host cells. The present study aimed to determine whether NHE3 activity is regulated by altering EGFR expression to affect Na+ absorption in TGEV-infected intestinal epithelial cells. Porcine intestinal epithelial cells were used as models for TGEV infection. The results showed that Na+ absorption and NHE3 expression levels decreased in TGEV-infected cells. Proliferation of TGEV within IPEC-J2 cells could be inhibited by treatment with the EGFR inhibitor AG1478 and knockdown; resulting in recovery of Na+ absorption in TGEV infected cells and increasing the activity and expression of NHE3. Moreover, we demonstrated that NHE3 activity was regulated through the EGFR/ERK pathway. Importantly, NHE3 mobility on the plasma membrane of TGEV infected cells was significantly weaker than that in normal cells, and EGFR inhibition and knockdown recovered this mobility. Our research indicated that NHE3 activity was negatively regulated by EGFR in TGEV-infected intestinal epithelial cells

Revisit of the Interaction between Holographic Dark Energy and Dark Matter

In this paper we investigate the possible direct, non-gravitational interaction between holographic dark energy (HDE) and dark matter. Firstly, we start with two simple models with the interaction terms $Q \propto \rho_{dm}$ and $Q \propto \rho_{de}$, and then we move on to the general form $Q \propto \rho_m^\alpha\rho_{de}^\beta$. The cosmological constraints of the models are obtained from the joint analysis of the present Union2.1+BAO+CMB+$H_0$ data. We find that the data slightly favor an energy flow from dark matter to dark energy, although the original HDE model still lies in the 95.4% confidence level (CL) region. For all models we find $c<1$ at the 95.4% CL. We show that compared with the cosmic expansion, the effect of interaction on the evolution of $\rho_{dm}$ and $\rho_{de}$ is smaller, and the relative increment (decrement) amount of the energy in the dark matter component is constrained to be less than 9% (15%) at the 95.4% CL. By introducing the interaction, we find that even when $c<1$ the big rip still can be avoided due to the existence of a de Sitter solution at $z\rightarrow-1$. We show that this solution can not be accomplished in the two simple models, while for the general model such a solution can be achieved with a large $\beta$, and the big rip may be avoided at the 95.4% CL.Comment: 26 pages, 9 figures, version accepted for publication in JCA

Analytical Computation of Information Rate for MIMO Channels

Information rate for discrete signaling constellations is significant. However, the computational complexity makes information rate rather difficult to analyze for arbitrary fading multiple-input multiple-output (MIMO) channels. An analytical method is proposed to compute information rate, which is characterized by considerable accuracy, reasonable complexity, and concise representation. These features will improve accuracy for performance analysis with criterion of information rate

Venture Capital and Chinese Firms&rsquo; Technological Innovation Capability: Effective Evaluation and Mechanism Verification

Making the financial industry a solider mainstay of the real economy is of great concern for China in the midst of economic reform. For China, leveraging venture capital (VC) to enhance a firm&rsquo;s technological innovation capability (TIC) is an important means of actualising its innovation and development strategy, as well as a must-do to realise sustainable development. In this study, firms that went public from 2010 to 2020 on the A-stock market were used as samples to study the effects of VC on TIC and the relevant mechanism based on the difference-in-differences (DID) method. As research findings show, VC can improve TIC through the medium of the internal incentive and external constraint easing effects. The contributory role of VC in TIC varies with firm size, ownership, and industry type. A range of robustness tests, including the PSM, variable substitution, and instrumental variable methods, further strengthened the reliability of the conclusions. This study can enlighten policymakers on how to implement comprehensive resource factor market reform to build a favourable innovation environment that materialises the role of marketisation