Fracture Mechanism of Brazilian Discs with Multiple Parallel Notches Using PFC2D

This study presents crack initiation, propagation and coalescenceat or near pre-existing open cracks in a numericalmodel under Brazilian test. Firstly, Particle Flow Code intwo dimensions (PFC2d) was calibrated with respect to thedata obtained from experimental laboratory tests to ensurethe conformity of the simulated numerical models response.Brazilian discs contain one, two, three, four, and five parallelcentred cracks (45° to the horizontal) under compressiveline loading. Models containing two and three cracks havedifferent joint spacing and joint configuration. In model consistingone flaws, tensile cracks initiated from notch tip andpropagates in direction of compressive loading till coalescewith model edge. By increasing the number of notch, first typeof tensile crack initiated at the tips of outer flaws and coalescedwith model edge. Also second type of tensile cracksinitiates from middle of inner flaws and coalesce with tip ofthe neighbouring flaws. The results show that joint spacingand joint configuration has important effect on the failure patternin model consisting two and three notch. Experimentaland numerical results rendered by other researchers showed agood agreement with the numerical results in the coalescencecharacteristics in cracked model. In addition, crack initiationand coalescence stresses in models were analyzed and comparedwith those in the single-flawed model

Energy-Economical Heuristically Based Control of Compass Gait Walking on Stochastically Varying Terrain

Investigation uses simulation to explore the inherent tradeoffs ofcontrolling high-speed and highly robust walking robots while minimizing energy consumption. Using a novel controller which optimizes robustness, energy economy, and speed of a simulated robot on rough terrain, the user can adjust their priorities between these three outcome measures and systematically generate a performance curveassessing the tradeoffs associated with these metrics

Latexin expression is downregulated in human gastric carcinomas and exhibits tumor suppressor potential

<p>Abstract</p> <p>Background</p> <p>Latexin, also known as endogenous carboxypeptidase inhibitor (CPI), has been found to inhibit mouse stem cell populations and lymphoma cell proliferation, demonstrating its potential role as a tumor suppressor. Our previous study also suggested a correlation between latexin expression and malignant transformation of immortalized human gastric epithelial cells. Here, we examined latexin expression in human gastric carcinomas and investigated the effect of differential latexin expression on proliferation of gastric cancer cells <it>in vitro </it>and <it>in vivo</it>.</p> <p>Methods</p> <p>Monoclonal antibody against human latexin was prepared and immunohistochemical analysis was performed to detect latexin expression in 41 paired gastric carcinomas and adjacent normal control tissues. Human gastric cancer cells MGC803 (latexin negative) stably transfected with LXN gene and BGC823 cells (latexin positive) stably transfected with antisense LXN gene were established for anchorage-dependent colony formation assay and tumorigenesis assay in nude mice. Differentially expressed genes in response to exogeneous latexin expression were screened using microarray analysis and identified by RT-PCR. Bisulfite sequencing was performed to analyze the correlation of the methylation status of LXN promoter with latexin expression in cell lines.</p> <p>Results</p> <p>Immunohistochemical analysis showed significantly reduced latexin expression in gastric carcinomas (6/41, 14.6%) compared to control tissues (31/41, 75.6%) (<it>P </it>< 0.05). Overexpression of LXN gene in MGC803 cells inhibited colony formation and tumor growth in nude mice. Conversely, BGC823 cells transfected with antisense LXN gene exhibited enhanced tumor growth and colony formation. Additionally, several tumor related genes, including Maspin, WFDC1, SLPI, S100P, and PDGFRB, were shown to be differentially expressed in MGC803 cells in response to latexin expression. Differential expression of Maspin and S100P was also identified in BGC823 cells while latexin expression was downregulated. Further bisulfite sequencing of the LXN gene promoter indicated CpG hypermethylation was correlated with silencing of latexin expression in human cells.</p> <p>Conclusions</p> <p>Latexin expression was reduced in human gastric cancers compared with their normal control tissues. The cellular and molecular evidences demonstrated the inhibitory effect of latexin in human gastric cancer cell growth and tumorigenicity. These results strongly suggest the possible involvement of latexin expression in tumor suppression.</p

Polycomb CBX7 Directly Controls Trimethylation of Histone H3 at Lysine 9 at the p16 Locus

BACKGROUND: H3K9 trimethylation (H3K9me3) and binding of PcG repressor complex-1 (PRC1) may play crucial roles in the epigenetic silencing of the p16 gene. However, the mechanism of the initiation of this trimethylation is unknown. METHODOLOGY/PRINCIPAL FINDINGS: In the present study, we found that upregulating the expression of PRC1 component Cbx7 in gastric cancer cell lines MGC803 and BGC823 led to significantly suppress the expression of genes within the p16-Arf-p15 locus. H3K9me3 formation was observed at the p16 promoter and Regulatory Domain (RD). CBX7 and SUV39H2 binding to these regions were also detectable in the CBX7-stably upregulated cells. CBX7-SUV39H2 complexes were observed within nucleus in bimolecular fluorescence complementation assay (BiFC). Mutations of the chromodomain or deletion of Pc-box abolished the CBX7-binding and H3K9me3 formation, and thus partially repressed the function of CBX7. SiRNA-knockdown of Suv39h2 blocked the repressive effect of CBX7 on p16 transcription. Moreover, we found that expression of CBX7 in gastric carcinoma tissues with p16 methylation was significantly lower than that in their corresponding normal tissues, which showed a negative correlation with transcription of p16 in gastric mucosa. CONCLUSION/SIGNIFICANCE: These results demonstrated for the first time, to our knowledge, that CBX7 could initiate H3K9me3 formation at the p16 promoter

Analysis of mine backfill behaviour and stability

Stable mine backfill is necessary for safe working conditions, maximum ore recovery and underground and surface stability. Due to the fact that cement costs can be a significant part of the operating costs in large underground mines, the consumption of cement (or binder) should be minimized. Therefore, developing a safe and economical backfilling method for the large underground mining operations is very significant.In this thesis, first laboratory tests and in-situ tests are implemented to determine backfill material properties and backfill stress distribution. The laboratory tests include high sulphide paste fill property tests and layered backfill tests. Second, a backfill finite element model is presented and it is validated by the results of laboratory tests and in-situ tests. Finally, by using this model, the following subjects are studied, (1) backfill stress distribution; (2) influences of backfill material properties and dimensions on backfill stability; (3) stress distribution of layered backfill; (4) optimum layered backfill. The results show that: (a) The variation of backfill material properties is quite large, the compressive strength of the layered backfill model is much higher than that of the non-layered backfill model, and the backfill vertical stress is much less than that anticipated by the formula, rhogH; (b) During the process of adjacent pillar recovery, the minor principal stress inside a backfill is tensile, and this tensile principal stress causes backfill failure and spalling near the exposed surfaces. The spalling size progressively increases with the height of the exposed surface, and a sliding zone creates and leads to backfill collapse; (c) Optimum backfill material should be high elastic modulus, high Poisson's ratio and low density. Optimum backfill size should be large depth and small width; (d) For layered backfill, no sliding zone occurs during the process of adjacent pillar recovery, so layered backfill can improve backfill stability. The optimum layered backfill should consist of strong layers distributed evenly with thicknesses of 1~2m and weak layers 2~2.5 times the thickness of the strong layers. This can save binder consumption by about 11%

An Experimental Study on Deformation Fractures of Fissured Rock around Tunnels in True Triaxial Unloads

Joints and cracks are frequently encountered in underground rock mass. During the process of tunnel excavations or other underground construction, the rock will be exposed suddenly, and such sudden unloading process will cause crack expansion and destabilize the rock structure. In order to investigate the crack behaviour during this process, a true triaxial loading apparatus with a computer-controlled electrohydraulic servosystem was established, and a series of true triaxial loading and unloading experiments was conducted by using concrete specimens containing inclined cracks with inclinations of 15°, 30°, 45°, 60°, and 75°. The stress-strain behavior and the failure property of rock models during unloading process were obtained, and, additionally, the coefficient of brittle stress drop was investigated. The uniaxial compression tests were simulated by using finite element method

The Fracture Characteristic of Three Collinear Cracks under True Triaxial Compression

The mechanical behavior of multicracks under compression has become a very important project in the field of fracture mechanics and rock mechanics. In this paper, experimental and numerical studies on the fracture property of three collinear cracks under compression were implemented. The specimens were a square concrete plate, and the cracks were made by a very thin film. The tests were conducted by using true triaxial loading device. In the numerical study, the Abaqus code was employed. The effect of crack orientation and the confining stress on cracked specimen compressive strength were investigated. The results show that the critical stresses of cracked specimens change with crack inclination angles, and, as the angle is 45°, the critical stress is the lowest; the critical stresses increase with the confining stresses

Numerical Study on Crack Propagation by Using Softening Model under Blasting

A mixed failure criterion, which combined the modified maximum principal stress criterion with the damage model of tensile crack softening, was developed to simulate crack propagation of rock under blasting loads. In order to validate the proposed model, a set of blasting models with a crack and a borehole with different incident angles with the crack were established. By using this model, the property of crack propagation was investigated. The linear equation of state (EOS) was used for rock, and the JWL EOS was applied to the explosive. In order to validate the numerical simulation results, experiments by using PMMA (polymethyl methacrylate) with a crack and a borehole were carried out. The charge structure and incident angle of the blasting experimental model were the same as those in the numerical models. The experiment results agree with the numerical simulation results