MOESM1 of Long non-coding RNA MIAT promotes growth and metastasis of colorectal cancer cells through regulation of miR-132/Derlin-1 pathway

Additional file 1: Figure S1. Down-regulation of MIAT inhibited SW480 cell proliferation, migration and invasion. SW480 cells were transfected with si-control, si-MIAT-1 or si-MIAT-2 for different time, (A) MIAT expression and cell viability was measured. SW480 cells were transfected with si-control, si-MIAT-1 or si-MIAT-2 for 72 h, (B) cell apoptosis, (C) cell migration and cell invasion was determined. **P < 0.01, compared to si-control

Synthesis, Structural Characterization, and Field-Effect Transistor Properties of <i>n</i>‑Channel Semiconducting Polymers Containing Five-Membered Heterocyclic Acceptors: Superiority of Thiadiazole Compared with Oxadiazole

Five-membered 1,3,4-oxadiazole (OZ) and 1,3,4-thiadiazole (TZ) heterocycle-based copolymers as active layer have long been ignored in solution-processable <i>n</i>-channel polymer field-effect transistors (PFETs) despite the long history of using OZ or TZ derivatives as the electron-injecting materials in organic light-emitting devices and their favorable electron affinities. Herein, we first report the synthesis and PFETs performance of two <i>n</i>-channel conjugated polymers bearing OZ- or TZ-based acceptor moieties, i.e., PNOZ and PNTZ, where simple thiophene units are utilized as the weak donors and additional alkylated-naphthalenediimides units are used as the second acceptors. A comparative study has been performed to reveal the effect of different heterocyclic acceptors on thermal properties, electronic properties, ordering structures, and carrier transport performance of the target polymers. It is found that both polymers possess low-lying LUMO values below −4.0 eV, indicating high electron affinity for both heterocycle-based polymers. Because of strong polarizable ability of sulfur atom in TZ heterocycle, PNTZ exhibits a red shift in maximal absorption and stronger molecular aggregation even in the diluted chlorobenzene solution as compared to the OZ-containing PNOZ. Surface morphological study reveals that a nodule-like surface with a rough surface morphology is observed clearly for PNOZ films, whereas PNTZ films display highly uniform surface morphology with well interconnected fiber-like polycrystalline grains. Investigation of PFETs performance indicates that both polymers afford air-stable <i>n</i>-channel transport characteristics. The uniform morphological structure and compact π–π stacking endow PNTZ with a high electron mobility of 0.36 cm² V^–1 s^–1, much higher than that of PNOZ (0.026 cm² V^–1 s^–1). These results manifest the feasibility in improving electron-transporting property simply by tuning heteroatom substitutes in <i>n</i>-channel polymers; further demostrate that TZ derivatives possess much superior potential for developing high-performance <i>n</i>-channel polymers compared to OZ derivatives

MOESM2 of Long non-coding RNA MIAT promotes growth and metastasis of colorectal cancer cells through regulation of miR-132/Derlin-1 pathway

Additional file 2: Figure S2.. Down-regulation of MIAT inhibited SW480 cell proliferation, migration and invasion by miR-132/Derlin-1 axis. SW480 cells were transfected with si-MIAT-2, miR-132 inhibitor and Derlin-1 shRNA (shRNA-Derlin-1) for 72 h, (A) cell viability, cell apoptosis, (B) cell migration and cell invasion was determined. **P < 0.01, compared to si-control. ##P < 0.01, compared to si-MIAT-2 + NC. & P < 0.01, compared to si-MIAT-2 + miR-132 inhibitor + shRNA

Number of unigenes related to RNAi.

<p>x-axis indicates the corresponding number of unigenes and y-axis indicates the specific unigenes related to RNAi. Forty-two unigenes coding for RNAi identified in the database, 26 of them were larger than 600 bp (61.90%) and 23 were more than 1 kb (54.76%).</p

Characteristics of the homology search of Illumina sequences against the NR database.

<p>(A) E-value distribution of the BLAST hits for each unique sequence with a cut-off E-value of 1.0E^-5. (B) Species distribution of the BLASTX results. The first hit of each sequence was used for further <i>in silico</i> analysis.</p

Number of unigenes related to intestinal digestive enzymes.

<p>x-axis indicates the corresponding number of unigenes and y-axis indicates the specific unigenes related to intestinal digestive enzymes. Three hundred and ninety-four protease-related unigenes were identified in <i>M</i>. <i>alternatus</i> Hope transcriptome. <b>(I) Number of specific unigenes related to serine proteases in intestinal digestive enzymes</b>. The icon indicates specific unigenes. The number in brackets indicates the corresponding number of unigenes.</p

Putative P450 genes identified in <i>Monochamus alternatus</i> Hope.

<p>Putative P450 genes identified in <i>Monochamus alternatus</i> Hope.</p

Number of unigenes related to immune-related molecules.

<p>x-axis indicates the corresponding number of unigenes and y-axis indicates the specific unigenes related to immune-related molecules. 478 unigenes were identified related to immune molecules and receptors in the transcriptome. This group contains 20 widely recognized immune factors. <b>(I) Number of specific unigenes related to IMD pathway in immune-related molecules. (II) Number of specific unigenes related to Toll pathway in immune-related molecules</b>. The icon indicates specific unigenes. The number in brackets indicates the corresponding number of unigenes.</p

Number of unigenes related to possible future insect control targets.

<p>x-axis indicates the corresponding number of unigenes and y-axis indicates the specific unigenes related to possible future insect control targets. 465 possible future insect control targets unigenes were found in <i>M</i>. <i>alternatus</i> Hope transcriptome. Among these, 117 unigenes had a length above 600 bp and 87 above 1 kb. <b>(I) Number of specific unigenes related to Serine Peptidase in possible future insect control targets</b>. The icon indicates specific unigenes. The number in brackets indicates the corresponding number of unigenes. Serine carboxypeptidase, serine-type endopeptidase and others belongs to the serine peptidases.</p

Unigenes length distribution.

<p>The y-axis number has been converted into logarithmic scale.</p