Immunohistochemical analysis of the expression of PNCK protein.

<p>PNCK is mainly localized within the nuclei and cytoplasmic. Immunostaining of the adjacent normal tissue samples(A) and the ccRCC tumor tissue samples(B) showed a sharp contrast between the negatively stained infiltrative tumorous area. (B): Negative or weak PNCK staining in cancerous tissue (400×). (C): Moderate PNCK staining in cancerous tissue(400×). (D): Strong PNCK staining in most of tumor cells (400×).</p

Real-time quantitative RT-PCR analysis of PNCK expression.

<p>The relative expression of PNCK mRNA in ccRCC tumor tissue samples was higher than that in the paired adjacent normal (N) tissue samples (n = 24, P<0.001). The bottom and the top of the box represent the 25th and the 75th percentile, respectively, and the band near the middle of the box is the 50th percentile (the median). The ends of the whiskers represent the 2.5th percentile and the 97.5th percentile.</p

Increased protein expression of PNCK in ccRCC.

<p>The relative protein expression of PNCK in ccRCC tumor (T) tissue samples was higher than that in the paired adjacent normal (N) tissue samples (n = 76, P<0.001). The bottom and top of the box are the lower and upper quartiles, and the band near the middle of the box is the median. The ends of the whiskers represent the 2.5th percentile and the 97.5th percentile. Four black stars represent the special value outliers.</p

Cox Regression analysis of the overall survival rates associated with different prognostic variables in patients with ccRCC.

<p>Cox Regression analysis of the overall survival rates associated with different prognostic variables in patients with ccRCC.</p

Correlation between PNCK expression and clinical pathologic features of the patients with clear cell renal cell carcinoma.

<p>Correlation between PNCK expression and clinical pathologic features of the patients with clear cell renal cell carcinoma.</p