Two Iterative algorithms for the matrix sign function based on the adaptive filtering technology

In this paper, two new efficient algorithms for calculating the sign function of the large-scale sparse matrix are proposed by combining filtering algorithm with Newton method and Newton Schultz method respectively. Through the theoretical analysis of the error diffusion in the iterative process, we designed an adaptive filtering threshold, which can ensure that the filtering has little impact on the iterative process and the calculation result. Numerical experiments are consistent with our theoretical analysis, which shows that the computational efficiency of our method is much better than that of Newton method and Newton Schultz method, and the computational error is of the same order of magnitude as that of the two methods.Comment: 18 pages,12 figure

A CRISPR/Cas12a-assisted rapid detection platform by biosensing the apxIVA of Actinobacillus pleuropneumoniae

Actinobacillus pleuropneumoniae is an important respiratory pig pathogen that causes substantial losses in the worldwide swine industry. Chronic or subclinical infection with no apparent clinical symptoms poses a challenge for preventing transmission between herds. Rapid diagnostics is important for the control of epidemic diseases. In this study, we formulated an A. pleuropneumoniae species-specific apxIVA-based CRISPR/Cas12a-assisted rapid detection platform (Card) that combines recombinase polymerase amplification (RPA) of target DNA and subsequent Cas12a ssDNase activation. Card has a detection limit of 10 CFUs of A. pleuropneumoniae, and there is no cross-reactivity with other common swine pathogens. The detection process can be completed in 1 h, and there was 100% agreement between the conventional apxIVA-based PCR and Card in detecting A. pleuropneumoniae in lung samples. Microplate fluorescence readout enables high-throughput use in diagnostic laboratories, and naked eye and lateral flow test readouts enable use at the point of care. We conclude that Card is a versatile, rapid, accurate molecular diagnostic platform suitable for use in both laboratory and low-resource settings

The mechanisms and factors that induce trained immunity in arthropods and mollusks

Besides dividing the organism’s immune system into adaptive and innate immunity, it has long been thought that only adaptive immunity can establish immune memory. However, many studies have shown that innate immunity can also build immunological memory through epigenetic reprogramming and modifications to resist pathogens’ reinfection, known as trained immunity. This paper reviews the role of mitochondrial metabolism and epigenetic modifications and describes the molecular foundation in the trained immunity of arthropods and mollusks. Mitochondrial metabolism and epigenetic modifications complement each other and play a key role in trained immunity

A novel HD-Zip I/C2H2-ZFP/WD-repeat complex regulates the size of spine base in cucumber

Fruit spine is an important trait in cucumber, affecting not only commercial quality, but also fruit smoothness, transportation and storage. Spine size is determined by a multi-cellular base. However, the molecular mechanism underlying the regulation of cucumber spine base remains largely unknown. Here, we report map-based cloning and characterization of a spine base size 1 (SBS1) gene, encoding a C2H2 zinc-finger transcription factor.Near-isogenic lines of cucumber were used to map, identify and quantify cucumber spine base size 1 (CsSBS1). Yeast-hybrid, bimolecular fluorescence complementation (BiFC), co-immunoprecipitation (Co-IP) and RNA-sequencing assays were used to explore the molecular mechanism of CsSBS1 in regulating spine base size development.CsSBS1 was specifically expressed in cucumber ovaries with particularly high expression in fruit spines. Overexpression of CsSBS1 resulted in large fruit spine base, while RNA-interference silencing of CsSBS1 inhibited the expansion of fruit spine base. Sequence analysis of natural cucumber accessions revealed that CsSBS1 was lost in small spine base accessions, resulting from a 4895 bp fragment deletion in CsSBS1 locus. CsSBS1 can form a trimeric complex with two positive regulators CsTTG1 and CsGL1 to regulate spine base development through ethylene signaling.A novel regulator network is proposed that the CsGL1/CsSBS1/CsTTG1 complex plays a significant role in regulating spine base formation and size, which offers a strategy for cucumber breeders to develop smooth fruit.This work was supported by the National Natural Science Foundation of China (31902020, 31972427), the Zhongyuan Youth Talent Program (ZYQR201912161), the Key Research Project of Henan institutions of higher learning (20A210015), the Program for Science & Technology Innovation Talents of Henan Province (21HASTIT038), and the Major Science and Technology Projects of Henan Province (201300111300).Peer reviewe

Reinvestigating the BB →{\to} PVPV decays by including the contributions from ϕB2{\phi}_{B2} with the perturbative QCD approach

Considering the $B$ mesonic wave function ${\phi}_{B2}$, the $B$ ${\to}$ $PV$ decays are restudied at the leading order for three scenarios using the perturbative QCD approach within the standard model, where $P$ $=$ ${\pi}$ and $K$, and $V$ denotes the ground $SU(3)$ vector mesons. It is found that contributions from ${\phi}_{B2}$ can enhance most branching ratios, and are helpful for improving the overall consistency of branching ratios between the updated calculations and available data, although there are still several discrepancies between the experimental and theoretical results.Comment: 50 page

Global calibration of multi-cameras with non-overlapping fields of view based on photogrammetry and reconfigurable target

Abstract Multi-camera vision systems are often needed to achieve large-scale and high-precision measurement because these systems have larger fields of view (FOV) than a single camera. Multiple cameras may have no or narrow overlapping FOVs in many applications, which pose a huge challenge to global calibration. This paper presents a global calibration method for multi-cameras without overlapping FOVs based on photogrammetry technology and a reconfigurable target. Firstly, two planar targets are fixed together and made into a long target according to the distance between the two cameras to be calibrated. The relative positions of the two planar targets can be obtained by photogrammetric methods and used as invariant constraints in global calibration. Then, the reprojection errors of target feature points in the two cameras’ coordinate systems are calculated at the same time and optimized by the Levenberg–Marquardt algorithm to find the optimal solution of the transformation matrix between the two cameras. Finally, all the camera coordinate systems are converted to the reference coordinate system in order to achieve global calibration. Experiments show that the proposed method has the advantages of high accuracy (the RMS error is 0.04?mm) and low cost and is especially suitable for on-site calibration

Research on Construction and SWRL Reasoning of Ontology of Maize Diseases

International audienceIn this paper, according to the characteristics of maize disease knowledge, OWL DL language was used to build maize diseases ontology, and the reasoning rule of maize diseases was defined by using the expressive ability of SWRL rule language.The author introduced several realizable reasoning functions,and achieved the diagnostic reasoning of maize disease knowledge by Jess inference engine.The results indicated that constructing the maize diseases ontology,and introducing SWRL rule into maize disease ontology provided an effective way for the construction of high-intelligent, shareable and reused maize disease knowledge database and diagnostic rule database

Zircon U-Pb dating and trace elements geochemistry of the Gaotan gabbro from Ziyang County, Shaanxi Province

Basic dike swarms are widely distributed in the Ziyang-Langao-Zhenping area of Ankang in Shaanxi Province and Zhuxi area in Hubei Province, intruding the Early Paleozoic strata which characterized by bimodal volcanic association. The gabbro dikes emplacement dated 410±5 Ma by the LA-ICP-MS zircon U-Pb dating. They show the patterns of enriched LREE with high fractionation of light and heavy REE, and enrichment of incompatible elements in the primitive mantle-normalized spider-diagrams with peaks of Ba, Nb and Ti and valleys of Pb and U. The ratios of Th/Nb is less than 0.11 and those of Nb/Zr more than 0.15, indicating that they were derived from enriched mantle source closely related to the plume. The ratios of Nb/U, Ce/Pb and concentration of U, Th, Pb and Ni in the gabbro dikes indicate that the pyroxenes and olivines were main fractional minerals in the fractional crystallization during the magma evolution, and they were not affected by contamination of continental crust from the mantle to crust. These helps to infer that the extensional tectonics developed constrainedly in the Early Paleozoic by the mantle plume activities that happened at the boundary of paleocontinent on the northern margin of Yangtze Plate, thus producing the large group of basic dike swarms in these areas. The deep thermodynamic condition is responsible for the crustal extension and end. Thus the age of 410±4.5 Ma not only represents the magma emplacement, but also the extension evolution history of this continent crust rift from beginning to end in Silurian