Additional file 1 of METTL3 promotes osteoblast ribosome biogenesis and alleviates periodontitis

Additional file 1: Figure S1. The effect of METTL3 on the rRNA stability. A The rRNA expression in METTL3-kncokdown cells after treating with 5 μg/mL ActD for 0–8 h was measured by qRT-PCR. n = 3. Figure S2. A, B The level of ROS was measured after METTL3 knockdown. n = 3. All data represent the mean ± SD. Figure S3. The effect of METTL3 knockdown on nucleolus, mTOR-Akt, and p53 pathway. A, B The nucleolar morphology of osteoblasts under LPS and osteogenic induction 3 days was assessed by immunocytochemistry. 20 nM actinomycin D (ActD) was the positive control of nucleolar stress. C The nucleolar number was detected by AgNOR staining. D, E The activation of p53 and AKT-mTOR signaling were examined by western blotting. *P < 0.05; **P < 0.01; ***P < 0.001. Figure S4. The effect of CHIR on the expression of Dkk3 and Sostdc1 in METTL3 knockdown cells. A, B The shCTRL and shMETTL3 cells were stimulated by LPS and osteogenic induction medium with or without CHIR. The mRNA expression of Dkk3 and Sostdc1 was detected by RT-qPCR. n = 3. All data represent the mean ± SD. *P < 0.05; ***P < 0.001. Figure S5. The effect of SAH and CHIR in periodontitis mice. A The proteins were evaluated in LPS-stimulated cells after stimulating with 5 μM SAH and 3 μM CHIR for 3 days. B Masson staining images of the periodontium. All data represent the mean ± SD. *P < 0.05; **P < 0.01. Table S1. Primer sequences for qRT-PCR. Table S2. Polysome profiling buffer