Additional file 2: Figure S2. of Characterization of four vaccine-related polioviruses including two intertypic type 3/type 2 recombinants associated with aseptic encephalitis

Phylogenetic trees based on 3D genomic regions of HEV-C generated by the neighbor-joining algorithm implemented in MEGA (version 6.06) using the Kimura two-parameter substitution model and 1,000 bootstrap pseudo-replicates. ▲strains isolated in this investigation; ● other PV3 strains. (DOC 682 kb

The expression of neural-specific marker containing TH, β-tubulinIII, MAP-2, NSE, Nestin and Lmx1α in hUC-MSCs induced 21 days was analyzed by immunofluorescence.

<p>Scale bar: 100 µm.</p

Immunohistochemistry of the TH-positive cells in the substantia nigra of the monkeys in two groups.

<p>Scale bar: 100 µm.</p

Expansion of hUC-MSCs cultured <i>in vitro</i>.

<p>(A) Morphology of hUC-MSCs primary cultured for 20 days. Scale bar: 40 µm. (B) Morphology of hUC-MSCs cultured at the 7^th generation. Scale bar: 100 µm.</p

Detection of NTN secretion in 24 h, 48 h, 72 h and 96 h of hUC-MSCs infected with Ad-NGFpreproNTN and control hUC-MSCs by ELISA (* p<0.05).

<p>Detection of NTN secretion in 24 h, 48 h, 72 h and 96 h of hUC-MSCs infected with Ad-NGFpreproNTN and control hUC-MSCs by ELISA (* p<0.05).</p

Primers sequences used in reverse transcription-polymerase chain reaction and Real-time PCR.

<p>Primers sequences used in reverse transcription-polymerase chain reaction and Real-time PCR.</p

Detction of the expression of Lmx1α by western blot.

<p>Lane 1, was loaded with the cell lysis infected with Ad-Lmx1α. Lane 2, was loaded with the medium infected with Ad-Lmx1α. Lane 3, was loaded with the cell lysis from control hUC-MSCs. Lane 4, was loaded with the medium from control hUC-MSCs.</p

The expression of neural-specific marker containing TH, β-tubulinIII, MAP-2, NSE, Nestin and Lmx1α in hUC-MSCs induced 7 days was analyzed by immunofluorescence.

<p>Scale bar: 100 µm.</p

Differentiation of hUC-MSCs into osteoblasts <i>in vitro</i>.

<p>(A, D) Morphology of hUC-MSCs cultured in osteogenic medium for 21 days. (B, E) <i>In vitro</i> differentiation of hUC-MSCs into osteoblasts, were shown by positive alizarin red (B) and ALP staining (E) of calcified extracellular matrix. (C, F) Staining was not observed in control hUC-MSCs cultured in growth medium. Scale bar: 100 µm.</p

Characterizaton and proliferation index of hUC-MSCs cultured in growth medium.

<p>(A) hUC-MSCs counterstain with BrdU. (B) hUC-MSCs counterstain with DAPI. Scale bar: 100 µm.</p