A Study of Flow Characteristics Near a Channel Confluence Using CCHE 2D/3D Models

Source: ICHE Conference Archive - https://mdi-de.baw.de/icheArchiv

Annealing novel nucleobase-lipids with oligonucleotides or plasmid DNA based on H-bonding or π-π interaction:Assemblies and transfections

Lipid derivatives of nucleoside analogs have been highlighted for their potential for effective gene delivery. A novel class of nucleobase-lipids are rationally designed and readily synthesized, comprising thymine/cytosine, an ester/amide linker and an oleyl lipid. The diversity of four nucleobase-lipids termed DXBAs (DOTA, DNTA, DOCA and DNCA) is investigated. Besides, DNCA is demonstrated to be an effective neutral transfection material for nucleic acid delivery, which enbles to bind to oligonucleotides via H-bonding and π-π stacking with reduced toxicity in vitro and in vivo. Several kinds of nucleic acid drugs including aptamer, ssRNA, antisense oligonucleotide, and plasmid DNAs can be delivered by DXBAs, especially DNCA. In particular, G4-aptamer AS1411 encapsulated by DNCA exhibits cellular uptake enhancement, lysosome degradation reduction, cell apoptosis promotion, cell cycle phase alteration in vitro and duration prolongation in vivo, resulting in significant anti-proliferative activity. Our results demonstrate that DNCA is a promising transfection agent for G4-aptamers and exhibites bright application prospects in the permeation improvement of single-stranded oligonucleotides or plasmid DNAs

ElectrochemicalN-demethylation of tropane alkaloids

A practical, efficient, and selective electrochemicalN-demethylation method of tropane alkaloids to their nortropane derivatives is described. Nortropanes, such as noratropine and norscopolamine, are important intermediates for the semi-synthesis of the medicines ipratropium or oxitropium bromide, respectively. Synthesis was performed in a simple home-made electrochemical batch cell using a porous glassy carbon electrode. The reaction proceeds at room temperature in one step in a mixture of ethanol or methanol and water. The method avoids hazardous oxidizing agents such as H(2)O(2)orm-chloroperbenzoic acid (m-CPBA), toxic solvents such as chloroform, as well as metal-based catalysts. Various key parameters were investigated in electrochemical batch or flow cells, and the optimized conditions were used in batch and flow-cells at gram scale to synthesize noratropine in high yield and purity using a convenient liquid-liquid extraction method without any need for chromatographic purification. Mechanistic studies showed that the electrochemicalN-demethylation proceeds by the formation of an iminium intermediate which is converted by water as the nucleophile. The optimized method was further applied to scopolamine, cocaine, benzatropine, homatropine and tropacocaine, showing that this is a generic way ofN-demethylating tropane alkaloids to synthesize valuable precursors for pharmaceutical products

Forward single‐cell sequencing into clinical application: Understanding of ageing and rejuvenation from clinical observation to single‐cell solution

Abstract Ageing is the part of natural biology lifespan and living pathology healthspan that is associated with impact from stress, environmental changes and diseases. The present Editorial emphasises the understanding of ageing biology and potential impact of rejuvenating interventions as a critical focus of clinical and translational medicine. The discovery and development of ageing‐associated biomarkers and therapeutic targets require understanding of the complexity of molecular metabolisms, immune responses and genetic regulations associated with ageing and rejuvenation. Transcriptomic patterns of circulating immune cells, red blood cells and platelets at single‐cell solution can be an important window to molecular mechanisms of ageing initiation and development. As part of programmes, deep understanding of ageing and rejuvenation correlations of clinical with single‐cell transcriptomics will provide more innovative opportunities to translate single‐cell RNA sequencing (scRNA‐seq) to clinical application

Electrochemical polymerization of carbon fibers and its effect on the interfacial properties of carbon reinforced epoxy resin composites

Electrochemical polymerization of carbon fibers and its effect on the interfacial properties of carbon reinforced epoxy resin composite

Borrelia miyamotoi sensu lato in Père David Deer and Haemaphysalis longicornis Ticks

By sequence analysis of 16S rRNA, flaB, p66, and glpQ, we identified Borrelia miyamotoi in 1 of 4 Père David deer (n = 43) seropositive for Borrelia spp. and 1.2% (3/244) of Haemaphysalis longicornis ticks from Dafeng Elk National Natural Reserve, China. Future studies should assess Borrelia pathogenesis in deer

Application and Development of Natural Plant Metabolite Oleanolic Acid in the Nano Era

Like other pentacyclic triterpenoids, oleanolic acid, a natural plant metabolite prevalent in plant peels, stems, and leaves, is regarded as a possible drug candidate. A growing number of studies have shown that oleanolic acid exhibits a variety of beneficial properties, including antiviral, anti-inflammatory, antioxidant, anticancer, and hepatoprotective effects. Additionally, the rapid advance of nanotechnology has dramatically improved oleanolic acid’s bioavailability and minimized its disadvantages, leading to unexpected changes in its pharmacological activity and use. Therefore, our aim was to review the progress of research on the distribution and biological properties of oleanolic acid in plants and to discuss new pharmaceutical approaches for oleanolic acid

A Novel Optimization Method for a Multi-Year Planning Scheme of an Active Distribution Network in a Large Planning Zone

Electric power distribution networks plays a significant role in providing continuous electrical energy to different categories of customers. In the context of the present advancements, future load expansion in the active distribution networks (ADNs) poses the key challenge of planning to be derived as a multi-stage optimization task, including the optimal expansion planning scheme optimization (EPSO). The planning scheme optimization is a multi-attribute decision-making issue with high complexity and solving difficulty, especially when it involves a large-scale planning zone. This paper proposes a novel approach of a multi-year planning scheme for the effective solution of the EPSO problem in large planning zones. The proposed approach comprises three key parts, where the first part covers two essential aspects, i.e., (i) suggesting a project condition set that considers the elements directly related to a group of specific conditions and requirements (collectively referred to as conditions) to ADN planning projects; and (ii) Developing a condition scoring system to evaluate planning projects. The second part of our proposed scheme is a quantization method of correlativity among projects based on two new concepts: contribution index (CI) and dependence index (DI). Finally, considering the multi-year rolling optimization, a detailed mathematical model of condition evaluation and spatiotemporal optimization sequencing of ADN planning projects is developed, where the evaluation and optimization are updated annually. The proposed model has been successfully validated on a practical distribution network located in Xiantao, China. The investigated case study and comparisons verify the various advantages, suitability, and effectiveness of the proposed planning scheme, consequently saving more than 10% of the investment compared with the existing implemented scheme