3 research outputs found
Evaluating Single-Cell DNA Damage Induced by Enhanced Radiation on a Gold Nanofilm Patch
Although
radiotherapy is a general oncology treatment and is often synergistically
applied with surgery and chemotherapy, it can cause side effects during
and after treatment. Gold nanoparticles were studied as a potential
material to enhance radiation to induce damage in cancer cells. However,
few studies have been conducted to examine the effects of gold nanofilm
on cell impairment under X-ray treatment. This paper describes a microfabrication-based
single-cell array platform to evaluate DNA damage induced by enhanced
X-ray radiation on gold nanofilm patches (GNFPs). Cancer cells were
patterned on GNFPs of different diameters and thicknesses, where each
cell was attached on one GNFP. The end-point DNA damage induced by
X-ray was examined in situ at the single-cell level using a halo assay.
The preliminary data demonstrated that the enhancement of DNA damage
was significantly related to the area and thickness of the GNFP. This
platform may be hopefully used to establish the mathematical relationships
among DNA damage, X-ray dosage, and thickness and area of the GNFP,
and further contribute to radiation dosage screening for personalized
radiotherapy