Dynamic 3D shape measurement based on the phase-shifting moir\'e algorithm

In order to increase the efficiency of phase retrieval,Wang proposed a high-speed moire phase retrieval method.But it is used only to measure the tiny object. In view of the limitation of Wang method,we proposed a dynamic three-dimensional (3D) measurement based on the phase-shifting moire algorithm.First, four sinusoidal fringe patterns with a pi/2 phase-shift are projected on the reference plane and acquired four deformed fringe patterns of the reference plane in advance. Then only single-shot deformed fringe pattern of the tested object is captured in measurement process.Four moire fringe patterns can be obtained by numerical multiplication between the the AC component of the object pattern and the AC components of the reference patterns respectively. The four low-frequency components corresponding to the moire fringe patterns are calculated by the complex encoding FT (Fourier transform) ,spectrum filtering and inverse FT.Thus the wrapped phase of the object can be determined in the tangent form from the four phase-shifting moire fringe patterns using the four-step phase shifting algorithm.The continuous phase distribution can be obtained by the conventional unwrapping algorithm. Finally, experiments were conducted to prove the validity and feasibility of the proposed method. The results are analyzed and compared with those of Wang method, demonstrating that our method not only can expand the measurement scope, but also can improve accuracy.Comment: 14 pages,5 figures. ams.or

Sensing polymer/DNA polyplex dissociation using quantum dot fluorophores

We characterized the dissociation of polymer/DNA polyplexes designed for gene delivery using water-soluble quantum dots (QDs). A pH-responsive pentablock copolymer was designed to form stable complexes with plasmid DNA via tertiary amine segments. Dissociation of the polyplex was induced using chloroquine where the efficiency of this process was sensed through changes in QD fluorescence. We found that increasing concentrations of pentablock copolymer and DNA led to quenching of QD fluorescence, while chloroquine alone had no measurable effect. The mechanism of quenching was elucidated by modeling the process as the combination of static and dynamic quenching from the pentablock copolymer and DNA, as well as self-quenching due the bridging of QDs. Tertiary amine homopolymers were also used to study the effect of chain length on quenching. Overall, these QDs were found to be highly effective at monitoring the dissociation of pentablock copolymer/DNA polyplexes in vitro and may have potential for studying the release of DNA within cells

Energetics of a solar flare and a coronal mass ejection generated by a hot channel eruption

Hot channels (HCs) are prevalent in the solar corona and play a critical role in driving flares and CMEs. In this paper, we estimate the energy contents of an X1.4 eruptive flare with a fast CME generated by a HC eruption on 2011 September 22. Originating from NOAA AR11302, the HC is the most dramatic feature in 131 and 94 {\AA} images observed by SDO/AIA. The flare is simultaneously observed by SDO/AIA, RHESSI, and STEREO-B/EUVI. The CME is simultaneously detected by the white-light coronagraphs of SOHO/LASCO and STEREO-B/COR1. Using multiwavelength and multiview observations of the eruption, various energy components of the HC, flare, and CME are calculated. The thermal and kinetic energies of the HC are (1.77$\pm$0.61)$\times10^{30}$ erg and (2.90$\pm$0.79)$\times10^{30}$ erg, respectively. The peak thermal energy of the flare and total radiative loss of SXR-emitting plasma are (1.63$\pm$0.04)$\times10^{31}$ erg and (1.03$-$1.31)$\times10^{31}$ erg, respectively. The ratio between the thermal energies of HC and flare is 0.11$\pm$0.03, suggesting that thermal energy of the HC is not negligible. The kinetic and potential energies of the CME are (3.43$\pm$0.94)$\times10^{31}$ erg and (2.66$\pm$0.49)$\times10^{30}$ erg, yielding a total energy of (3.69$\pm$0.98)$\times10^{31}$ erg for the CME. Continuous heating of the HC is required to balance the rapid cooling by heat conduction, which probably originate from intermittent magnetic reconnection at the flare current sheet. Our investigation may provide insight into the buildup, release, and conversion of energies in large-scale solar eruptions.Comment: 11 pages, 9 figures, accepted for publication by Ap