A novel two-step recombinogenic engineering method for bacterial artificial chromosome manipulation

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Physiological responses to swimming fatigue of juvenile white-leg shrimp Litopenaeus vannamei exposed to different current velocities, temperatures and salinities

Swimming performance is one of the crucial factors determining the lifestyle and survival of Penaeid shrimps. This study examined under controlled laboratory conditions, the physiological responses to swimming fatigue of juvenile white-leg shrimp Litopenaeus vannamei (8.85 ± 0.05 cm TL) exposed to different current velocities, temperatures and salinities factors which have been correlated with their swimming performance. The swimming endurance of juveniles decreased as current velocity increasedfrom 5.41 to 11.47 cm s-1 at any of the temperatures and salinities tested. Exercise to fatigue led to severe loss of serum total protein concentration (PC) and serum glucose level (SG) in L. vannamei exposed to different current velocities, temperatures and salinities (P < 0.05). Moreover, decrease of PC and SG in fatigued shrimp varied with current velocity, temperature and salinity. The results showed that the mobilization of protein and glucose in response to swimming fatigue was rapidly diminished and suggest how physiological responses to swimming fatigue of juvenile white-leg shrimp L. vannamei exposed to different current velocity, temperature and salinity may determine their swimming performances.Key words: Litopenaeus vannamei, swimming fatigue, current velocities, temperatures, salinities

Identification of potential drug resistant mutations in HIV-1 infected patients with long-term undetectable viral load after receiving HAART

This journal suppl. entitled: HIV DART 2010 ... Final Program and Abstract BookSession - Drug Resistance and ModelingConference Theme: Frontiers in drug development for in antiretroviral therapiesBACKGROUND: This study aimed to identify potential drug resistant mutations in proviral DNA in HIV patients with long-term undetectable viral load after receiving HAART. METHOD: Peripheral blood mononuclear cells (PBMC) were collected from 45 patients twice per year from 2005 to 2009. These patients have been receiving effective highly active antiretroviral therapy …published_or_final_versio

Electrocatalytic Activity of Electropolymerized Cobalt Tetraaminophthalocyanine Film Modified Electrode towards 6-Mercaptopurine and 2-Mercaptobenzimidazole

The electrocatalytic activity of electropolymerized cobalt tetraaminophthalocyanine (poly-CoTAPc) film modified on the glassy carbon electrode (GCE) towards 6-mercaptopurine (6MP) and 2-Mercaptobenzimidazole (MBI) was studied. Comparing with the case at the unmodified GCE, the poly-CoTAPc film decreased the overpotential of oxidation of 6MP (1.0 × 10–3 mol L–1) and MBI (1.0×10–3 mol L–1) by 335 and 189mV, respectively, and increased the peak current by about 3 and 2 times, respectively, while the reduction potential of the disulphide product of previous oxidation of 6MP shifted positively by 312 mV and the peak current increased by 40 times in 0.1 mol L–1 phosphate buffer solution (PBS, pH = 2.0). The poly-CoTAPc film exhibited efficiently electrocatalytic activity for 6MP and MBI with relatively high sensitivity, stability and long-life.Keywords: Electrocatalysis, 6-mercaptopurine, 2-mercaptobenzimidazole, Cobalt Tetraaminophthalocyanine, Electropolymeric Fil

Networked Control Systems: The Communication Basics and Control Methodologies

As an emerging research field, networked control systems have shown the increasing importance and attracted more and more attention in the recent years. The integration of control and communication in networked control systems has made the design and analysis of such systems a great theoretical challenge for conventional control theory. Such an integration also makes the implementation of networked control systems a necessary intermediate step towards the final convergence of control, communication, and computation. We here introduce the basics of networked control systems and then describe the state-of-the-art research in this field. We hope such a brief tutorial can be useful to inspire further development of networked control systems in both theory and potential applications

Power distance orientation alleviates the beneficial effects of empowering leadership on actors' work engagement via negative affect and sleep quality

Although many studies have explored the benefits of empowering leadership for followers, the beneficial effect of such behavior for actors who demonstrate empowering leadership has been overlooked. Applying conservation of resources theory, we propose and test a model that determines why and when empowering leadership benefits actors. We use an experience sampling survey to examine the effect of empowering leadership on actors’ daily work engagement. In particular, we focus on the moderating role of power distance orientation and the mediating roles of negative affect and sleep quality, which operate sequentially. The results based on responses from 160 supervisors in two Chinese organizations indicated that empowering leadership in the morning was negatively related to negative affect in the afternoon and positively related to sleep quality at night and next-day work engagement. The strength of this beneficial effect was moderated by power distance orientation, such that supervisors with a high degree of power distance orientation obtained fewer benefits from empowering leadership than those with a low degree of power distance orientation. The theoretical and practical implications of these findings for the leadership, affect, sleep, power distance, and conservation of resources literatures are discussed

Finite-temperature Gutzwiller projection for strongly correlated electron systems

We generalized the Gutzwiller projectional variational method for the ground state of strongly correlated electron systems to the case of finite temperature. Under the Gutzwiller approximation, we show that this maps to a finite temperature renormalized mean-field theory. As one of the key ingredients in the theory, we obtained an explicit expression of the projection entropy or the entropy change due to the projection. We illustrate the application of the theory to the Anderson impurity problem and the half-filled Hubbard model and compare the theory to more elaborate techniques. We find qualitative agreement. The theory can be applied to a wide variety of Hubbard, t-J, and Anderson impurity models. © 2010 The American Physical Society.published_or_final_versio