Minimally invasive surgery for uterine fibroids

The incidence of uterine fibroids, which comprise one of the most common female pelvic tumors, is almost 70–75% forwomen of reproductive age. With the development of surgical techniques and skills, more individuals prefer minimallyinvasive methods to treat uterine fibroids. There is no doubt that minimally invasive surgery has broad use for uterinefibroids. Since laparoscopic myomectomy was first performed in 1979, more methods have been used for uterine fibroids,such as laparoscopic hysterectomy, laparoscopic radiofrequency volumetric thermal ablation, and uterine artery embolization,and each has many variations. In this review, we compared these methods of minimally invasive surgery for uterinefibroids, analyzed their benefits and drawbacks, and discussed their future development

HPV Genotyping by Molecular Mapping of Tissue Samples in Vaginal Intraepithelial Neoplasia (VaIN) and Vaginal Squamous Cell Carcinoma (VaSCC)

HPV genotypes were determined in 63 vaginal intraepithelial neoplasia (VaIN) and 7 vaginal squamous cell carcinomas (VaSCC). Of these, 37 cases had VaIN alone, and 26 cases had both VaIN and cervical intraepithelial neoplasia (CIN) or condyloma. HPV typing was performed in scraped cells by Genosearch-31 (GS-31) and in the archived tissues by uniplex E6/E7 PCR. In a total of 49 VaIN1, 17 VaIN2/3, and 7 VaSCC tissues, the prevalence of HPV was 91.2% in VaIN (VaIN1: 87.8%, VaIN2/3: 100%) and 85.7% in VaSCC. Comparing HPV results in scraped cell and tissue, 46.2% of high-risk (HR) types and 68.1% of any HPV types that had been identified in cell samples were not present in corresponding tissues. HPV types in VaIN and CIN lesions differed in 92.3% (24/26) of cases with multiple lesions. These results suggest that there are many preclinical HPV infections in the vagina or the cervix, and VaIN and CIN are independently developed. The manual microdissection procedure of tissue revealed one HPV type in one lesion. Seventeen HPV types, including high-risk (HR), possible high-risk (pHR), and low-risk (LR), were identified in 43 VaIN1 lesions. In higher grade lesions, six HR (HPV16, 18, 51, 52, 56, 58), one pHR (HPV66), and one LR (HPV42) HPV types were identified in 17 VaIN2/3, and six HPV types, including HPV16, 45, 58, and 68 (HR), and HPV53 and 67 (pHR), were detected in each case of VaSCC. The vagina appears to be the reservoir for any mucosal HPV type, and HR- or pHR-HPV types are causative agents for vaginal malignancies

Cytosine–phosphate–guanine oligodeoxynucleotides alleviate radiation-induced kidney injury in cervical cancer by inhibiting DNA damage and oxidative stress through blockade of PARP1/XRCC1 axis

Abstract Background Radiotherapy can cause kidney injury in patients with cervical cancer. This study aims to investigate the possible molecular mechanisms by which CpG-ODNs (Cytosine phosphate guanine-oligodeoxynucleotides) regulate the PARP1 (poly (ADP-ribose) polymerase 1)/XRCC1 (X-ray repair cross-complementing 1) signaling axis and its impact on radiation kidney injury (RKI) in cervical cancer radiotherapy. Methods The GSE90627 dataset related to cervical cancer RKI was obtained from the Gene Expression Omnibus (GEO) database. Bioinformatics databases and R software packages were used to analyze the target genes regulated by CpG-ODNs. A mouse model of RKI was established by subjecting C57BL/6JNifdc mice to X-ray irradiation. Serum blood urea nitrogen (BUN) and creatinine levels were measured using an automated biochemical analyzer. Renal tissue morphology was observed through HE staining, while TUNEL staining was performed to detect apoptosis in renal tubular cells. ELISA was conducted to measure levels of oxidative stress-related factors in mouse serum and cell supernatant. An in vitro cell model of RKI was established using X-ray irradiation on HK-2 cells for mechanism validation. RT-qPCR was performed to determine the relative expression of PARP1 mRNA. Cell proliferation activity was assessed using the CCK-8 assay, and Caspase 3 activity was measured in HK-2 cells. Immunofluorescence was used to determine γH2AX expression. Results Bioinformatics analysis revealed that the downstream targets regulated by CpG-ODNs in cervical cancer RKI were primarily PARP1 and XRCC1. CpG-ODNs may alleviate RKI by inhibiting DNA damage and oxidative stress levels. This resulted in significantly decreased levels of BUN and creatinine in RKI mice, as well as reduced renal tubular and glomerular damage, lower apoptosis rate, decreased DNA damage index (8-OHdG), and increased levels of antioxidant factors associated with oxidative stress (SOD, CAT, GSH, GPx). Among the CpG-ODNs, CpG-ODN2006 had a more pronounced effect. CpG-ODNs mediated the inhibition of PARP1, thereby suppressing DNA damage and oxidative stress response in vitro in HK-2 cells. Additionally, PARP1 promoted the formation of the PARP1 and XRCC1 complex by recruiting XRCC1, which in turn facilitated DNA damage and oxidative stress response in renal tubular cells. Overexpression of either PARP1 or XRCC1 reversed the inhibitory effects of CpG-ODN2006 on DNA damage and oxidative stress in the HK-2 cell model and RKI mouse model. Conclusion CpG-ODNs may mitigate cervical cancer RKI by blocking the activation of the PARP1/XRCC1 signaling axis, inhibiting DNA damage and oxidative stress response in renal tubule epithelial cells

Exploring the Spatio-Temporal and Behavioural Variations in Taxi Travel Based on Big Data during the COVID-19 Pandemic: A Case Study of New York City

The COVID-19 pandemic has caused severe social and economic chaos worldwide. To explore the impact of the COVID-19 pandemic on the travel patterns of residents, we analysed taxi trajectory data and COVID-19 pandemic data from New York City. Pearson coefficients, which were −0.7139, −0.8041, and −0.7046 during the three waves of the COVID-19, revealed a significant negative correlation between confirmed cases and taxi trips. Moran’s I was higher in drop-off areas than in pick-up areas, indicating a stronger spatial autocorrelation in drop-off areas during the study period. The hotspots of travel destinations had changed by spatial clustering, and variations in origin–destination distribution were obvious after the pandemic. Comparison of temporal and spatial dimensions before and after the pandemic revealed that strict epidemic policies directly affected travel. For instance, a week after the restrictions the taxi journeys plummeted by 95.3%, and their spatial and temporal patterns also changed. Once the anti-epidemic policy was eased or lifted, the taxi travel recovered, whereas, notably the new Omicron wave did not cause dramatic changes in taxi journeys. Despite this, travel spatial and temporal patterns did not return to pre-pandemic levels by the end of March 2022, the taxi journeys remained below half the pre-pandemic level. This study identified the profound impact of the COVID-19 outbreak on travel patterns and revealed distinct variations in behavioural responses during the pandemic and in response to subsequent policies. Strengthening targeted epidemic prevention and control measures are required to improve the balance between anti-epidemic policies and implementation efforts, that will facilitate the recovery of urban transport, work, and lifestyle of residents

Quantitative Research Methods of Linguistic Niche and Cultural Sustainability

Building Sustainable Cities and Human Communities is one of the 17 Sustainable Development Goals of the United Nations. And the sustainability of culture plays an important role in the sustainable development of cities and human communities. Language is an important carrier of culture, and the sustainability of language is the key factor in the sustainability of culture. How to measure the sustainability of language and its niche is key to achieving sustainable cities and communities. This paper systematically summarized the concept of niche and the theory of ecolinguistics as a theoretical basis for the quantitative study of the linguistic niche, and at the same time, the methods of niche measurement were summarized to provide mathematical support for the quantitative study of the linguistic niche. The Shannon-Wiener index and Pianka index were used to quantitatively study a particular linguistic niche for the first time, based on the use of Hmong and Mandarin in Jianhe County, Guizhou Province, China. The results showed that in the temporal dimension, the niche overlap indexes of Hmong and Mandarin were all above 0.9 in the sample villages, but in the spatial dimension, the niche overlap indexes of both languages were between 0.5 and 0.6. The spatial niche separation moderated the high temporal niche overlap, which made the two languages’ spatio-temporal niche overlap at a medium-high level. The quantitative study of a linguistic niche proved helpful in quantifying the level of sustainable development of language and culture, thus providing timely, accurate, and dynamic reference data to inform macro-control policies on the sustainable development of cities and human communities

Pavement Maintenance Decision Making Based on Optimization Models

Pavement maintenance prioritization considering both quality and cost is an important decision-making problem. In this paper, the actual pavement condition index of city roads was calculated using municipal patrol data. A linear optimization model that maximized maintenance quality with limited maintenance costs and a multi-objective optimization model that maximized maintenance quality while minimizing maintenance costs were developed based on the pavement condition index. These models were subsequently employed in making decisions for actual pavement maintenance using sequential quadratic programming and a genetic algorithm. The results showed that the proposed decision-making models could effectively address actual pavement maintenance issues. Additionally, the results of the single-objective linear optimization model verified that the multiobjective optimization model was accurate. Thus, they could provide optimal pavement maintenance schemes for roads according to actual pavement conditions. The reliability of the models was investigated by analyzing their assumptions and validating their optimization results. Furthermore, their applicability in pavement operation-related decision making and preventive maintenance for roads of different grades was confirmed

Multifunctional Microneedle Patches via Direct Ink Drawing of Nanocomposite Inks for Personalized Transdermal Drug Delivery

Additive manufacturing, commonly known as 3D printing, allows decentralized drug fabrication of orally administered tablets. Microneedles are comparatively favorable for self-administered transdermal drug delivery with improved absorption and bioavailability. Due to the cross-scale geometric characteristics, 3D-printed microneedles face a significant trade-off between the feature resolution and production speed in conventional layer-wise deposition sequences. In this study, we introduce an economical and scalable direct ink drawing strategy to create drug-loaded microneedles. A freestanding microneedle is efficiently generated upon each pneumatic extrusion and controlled drawing process. Sharp tips of ∼5 μm are formed with submillimeter nozzles, representing 2 orders of magnitude improved resolution. As the key enabler of this fabrication strategy, the yield-stress fluid inks are formulated by simply filling silica nanoparticles into regular polymer solutions. The approach is compatible with various microneedles based on dissolvable, biodegradable, and nondegradable polymers. Various matrices are readily adopted to adjust the release behaviors of the drug-loaded microneedles. Successful fabrication of multifunctional patches with heterogeneously integrated microneedles allows the treatment of melanoma via synergistic photothermal therapy and combination chemotherapy. The personalized patches are designed for cancer severity to achieve high therapeutic efficacy with minimal side effects. The direct ink drawing reported here provides a facile and low-cost fabrication strategy for multifunctional microneedle patches for self-administering transdermal drug delivery

Multifunctional Microneedle Patches via Direct Ink Drawing of Nanocomposite Inks for Personalized Transdermal Drug Delivery

Additive manufacturing, commonly known as 3D printing, allows decentralized drug fabrication of orally administered tablets. Microneedles are comparatively favorable for self-administered transdermal drug delivery with improved absorption and bioavailability. Due to the cross-scale geometric characteristics, 3D-printed microneedles face a significant trade-off between the feature resolution and production speed in conventional layer-wise deposition sequences. In this study, we introduce an economical and scalable direct ink drawing strategy to create drug-loaded microneedles. A freestanding microneedle is efficiently generated upon each pneumatic extrusion and controlled drawing process. Sharp tips of ∼5 μm are formed with submillimeter nozzles, representing 2 orders of magnitude improved resolution. As the key enabler of this fabrication strategy, the yield-stress fluid inks are formulated by simply filling silica nanoparticles into regular polymer solutions. The approach is compatible with various microneedles based on dissolvable, biodegradable, and nondegradable polymers. Various matrices are readily adopted to adjust the release behaviors of the drug-loaded microneedles. Successful fabrication of multifunctional patches with heterogeneously integrated microneedles allows the treatment of melanoma via synergistic photothermal therapy and combination chemotherapy. The personalized patches are designed for cancer severity to achieve high therapeutic efficacy with minimal side effects. The direct ink drawing reported here provides a facile and low-cost fabrication strategy for multifunctional microneedle patches for self-administering transdermal drug delivery

Multifunctional Microneedle Patches via Direct Ink Drawing of Nanocomposite Inks for Personalized Transdermal Drug Delivery

Additive manufacturing, commonly known as 3D printing, allows decentralized drug fabrication of orally administered tablets. Microneedles are comparatively favorable for self-administered transdermal drug delivery with improved absorption and bioavailability. Due to the cross-scale geometric characteristics, 3D-printed microneedles face a significant trade-off between the feature resolution and production speed in conventional layer-wise deposition sequences. In this study, we introduce an economical and scalable direct ink drawing strategy to create drug-loaded microneedles. A freestanding microneedle is efficiently generated upon each pneumatic extrusion and controlled drawing process. Sharp tips of ∼5 μm are formed with submillimeter nozzles, representing 2 orders of magnitude improved resolution. As the key enabler of this fabrication strategy, the yield-stress fluid inks are formulated by simply filling silica nanoparticles into regular polymer solutions. The approach is compatible with various microneedles based on dissolvable, biodegradable, and nondegradable polymers. Various matrices are readily adopted to adjust the release behaviors of the drug-loaded microneedles. Successful fabrication of multifunctional patches with heterogeneously integrated microneedles allows the treatment of melanoma via synergistic photothermal therapy and combination chemotherapy. The personalized patches are designed for cancer severity to achieve high therapeutic efficacy with minimal side effects. The direct ink drawing reported here provides a facile and low-cost fabrication strategy for multifunctional microneedle patches for self-administering transdermal drug delivery