Small tourism enterprises (STMs) and sustainable tourism development in Lao PDR: a GSEM-based analysis

In many developing countries, small tourism enterprises took the central role in tourism development from economic, social and cultural perspectives. This study clarifies, (1) what kinds of firms are more concerned about such sustainable tourism development, (2) what factors are associated with these types of firms, and (3) how to encourage more firms to be engaged in promoting sustainable tourism. For this purpose, we conducted a questionnaire survey in two major tourist destinations, Luang Prabang and Pakse in Lao PDR, to tourism-related business owners, and obtained 177 valid questionnaires. This is the first study in literature to target Lao PDR in the above context. Lao and foreign enterprises were first compared from some of the above perspectives and then a generalized structural equation model (GSEM) is adopted to capture complicated cause-effect relationships in answering the above questions by reflecting original features of data included in a more scientific way

農村観光による条件不利地域の発展 : 小規模企業者と農村観光客の視点

内容の要約広島大学(Hiroshima University)博士(学術)Doctor of Philosophydoctora

Rethinking Attention-Based Multiple Instance Learning for Whole-Slide Pathological Image Classification: An Instance Attribute Viewpoint

Multiple instance learning (MIL) is a robust paradigm for whole-slide pathological image (WSI) analysis, processing gigapixel-resolution images with slide-level labels. As pioneering efforts, attention-based MIL (ABMIL) and its variants are increasingly becoming popular due to the characteristics of simultaneously handling clinical diagnosis and tumor localization. However, the attention mechanism exhibits limitations in discriminating between instances, which often misclassifies tissues and potentially impairs MIL performance. This paper proposes an Attribute-Driven MIL (AttriMIL) framework to address these issues. Concretely, we dissect the calculation process of ABMIL and present an attribute scoring mechanism that measures the contribution of each instance to bag prediction effectively, quantifying instance attributes. Based on attribute quantification, we develop a spatial attribute constraint and an attribute ranking constraint to model instance correlations within and across slides, respectively. These constraints encourage the network to capture the spatial correlation and semantic similarity of instances, improving the ability of AttriMIL to distinguish tissue types and identify challenging instances. Additionally, AttriMIL employs a histopathology adaptive backbone that maximizes the pre-trained model's feature extraction capability for collecting pathological features. Extensive experiments on three public benchmarks demonstrate that our AttriMIL outperforms existing state-of-the-art frameworks across multiple evaluation metrics. The implementation code is available at https://github.com/MedCAI/AttriMIL.Comment: 10 pages, 8 figure

H2ASeg: Hierarchical Adaptive Interaction and Weighting Network for Tumor Segmentation in PET/CT Images

Positron emission tomography (PET) combined with computed tomography (CT) imaging is routinely used in cancer diagnosis and prognosis by providing complementary information. Automatically segmenting tumors in PET/CT images can significantly improve examination efficiency. Traditional multi-modal segmentation solutions mainly rely on concatenation operations for modality fusion, which fail to effectively model the non-linear dependencies between PET and CT modalities. Recent studies have investigated various approaches to optimize the fusion of modality-specific features for enhancing joint representations. However, modality-specific encoders used in these methods operate independently, inadequately leveraging the synergistic relationships inherent in PET and CT modalities, for example, the complementarity between semantics and structure. To address these issues, we propose a Hierarchical Adaptive Interaction and Weighting Network termed H2ASeg to explore the intrinsic cross-modal correlations and transfer potential complementary information. Specifically, we design a Modality-Cooperative Spatial Attention (MCSA) module that performs intra- and inter-modal interactions globally and locally. Additionally, a Target-Aware Modality Weighting (TAMW) module is developed to highlight tumor-related features within multi-modal features, thereby refining tumor segmentation. By embedding these modules across different layers, H2ASeg can hierarchically model cross-modal correlations, enabling a nuanced understanding of both semantic and structural tumor features. Extensive experiments demonstrate the superiority of H2ASeg, outperforming state-of-the-art methods on AutoPet-II and Hecktor2022 benchmarks. The code is released at https://github.com/JinPLu/H2ASeg.Comment: 10 pages,4 figure

A Localization-to-Segmentation Framework for Automatic Tumor Segmentation in Whole-Body PET/CT Images

Fluorodeoxyglucose (FDG) positron emission tomography (PET) combined with computed tomography (CT) is considered the primary solution for detecting some cancers, such as lung cancer and melanoma. Automatic segmentation of tumors in PET/CT images can help reduce doctors' workload, thereby improving diagnostic quality. However, precise tumor segmentation is challenging due to the small size of many tumors and the similarity of high-uptake normal areas to the tumor regions. To address these issues, this paper proposes a localization-to-segmentation framework (L2SNet) for precise tumor segmentation. L2SNet first localizes the possible lesions in the lesion localization phase and then uses the location cues to shape the segmentation results in the lesion segmentation phase. To further improve the segmentation performance of L2SNet, we design an adaptive threshold scheme that takes the segmentation results of the two phases into consideration. The experiments with the MICCAI 2023 Automated Lesion Segmentation in Whole-Body FDG-PET/CT challenge dataset show that our method achieved a competitive result and was ranked in the top 7 methods on the preliminary test set. Our work is available at: https://github.com/MedCAI/L2SNet.Comment: 7 pages,3 figure

D3GU: Multi-Target Active Domain Adaptation via Enhancing Domain Alignment

Unsupervised domain adaptation (UDA) for image classification has made remarkable progress in transferring classification knowledge from a labeled source domain to an unlabeled target domain, thanks to effective domain alignment techniques. Recently, in order to further improve performance on a target domain, many Single-Target Active Domain Adaptation (ST-ADA) methods have been proposed to identify and annotate the salient and exemplar target samples. However, it requires one model to be trained and deployed for each target domain and the domain label associated with each test sample. This largely restricts its application in the ubiquitous scenarios with multiple target domains. Therefore, we propose a Multi-Target Active Domain Adaptation (MT-ADA) framework for image classification, named D3GU, to simultaneously align different domains and actively select samples from them for annotation. This is the first research effort in this field to our best knowledge. D3GU applies Decomposed Domain Discrimination (D3) during training to achieve both source-target and target-target domain alignments. Then during active sampling, a Gradient Utility (GU) score is designed to weight every unlabeled target image by its contribution towards classification and domain alignment tasks, and is further combined with KMeans clustering to form GU-KMeans for diverse image sampling. Extensive experiments on three benchmark datasets, Office31, OfficeHome, and DomainNet, have been conducted to validate consistently superior performance of D3GU for MT-ADA.Comment: Accepted Poster at WACV 202

Globularifolin inhibits CAMA-1 human breast cancer cell line via cell cycle arrest, apoptosis and inhibition of PI3K/AKT signalling pathway

Purpose: To investigate the anticancer activity of globularifolin against CAMA-1 breast cancer cells.Methods: The viability of CAMA-1 cells was assessed by MTT assay. DAPI and annexin V/PI staining were used for the determination of apoptotic cell death. Flow cytometry was employed for cell cycle analysis, while wound healing and immunoblotting assays were used to measure cell migration and protein expression, respectively.Results: Globularifolin decreased the viability of CAMA-1 breast cancer cells dose-dependently, with half-maximal inhibitory concentration (IC50) of 10 μM, relative to its IC50 of 65 μM against non-cancerous CMMT breast cells. It also initiated apoptotic cell death and cell cycle arrest. Moreover, it inhibited the migration of CAMA-1 breast cancer cells, and PI3K/AKT signalling cascade.Conclusion: These results suggest that globularifolin has promising potential for use in the treatment of breast cancer.Keywords: Breast cancer, Globularifolin, Apoptosis, Cell cycle, Cell migratio

Boundary-aware Contrastive Learning for Semi-supervised Nuclei Instance Segmentation

Semi-supervised segmentation methods have demonstrated promising results in natural scenarios, providing a solution to reduce dependency on manual annotation. However, these methods face significant challenges when directly applied to pathological images due to the subtle color differences between nuclei and tissues, as well as the significant morphological variations among nuclei. Consequently, the generated pseudo-labels often contain much noise, especially at the nuclei boundaries. To address the above problem, this paper proposes a boundary-aware contrastive learning network to denoise the boundary noise in a semi-supervised nuclei segmentation task. The model has two key designs: a low-resolution denoising (LRD) module and a cross-RoI contrastive learning (CRC) module. The LRD improves the smoothness of the nuclei boundary by pseudo-labels denoising, and the CRC enhances the discrimination between foreground and background by boundary feature contrastive learning. We conduct extensive experiments to demonstrate the superiority of our proposed method over existing semi-supervised instance segmentation methods.Comment: 12 pages, 3 figures, 6 table