Effect of Qilongtoutong granule on calcitonin gene-related peptide, beta-endorphin, serotonin, dopamine, and noradrenalin in migraine model rats and mice

AbstractObjectiveTo study the effect of Qilongtoutong granule (QLTT) on plasma calcitonin gene-related peptide (CGRP), beta-endorphin (β-EP), 5-HT, dopamine (DA), noradrenalin (NE), and blood viscosity in migraine model rats and mice.MethodsBoth the acute blood stasis model group and nitroglycerin-induced migraine model group included 60 Sprague-Dawley rats. The reserpine-reduced model group had 60 Kunming mice. Rats from each test were grouped into normal control group, model group, Zhengtian pill (ZTP) group, and high, moderate, or low-dose QLTT groups. In the acute blood stasis model test, after gavage for 7 days, rats were given 0.8 mL/kg adrenaline hydrochloride subcutaneously twice, and kept in ice water for 5 min. After fasting for 12 h, rats were anesthetized and blood samples were collected for detection of blood viscosity. In the nitroglycerin-induced migraine group, after gavage for 7 days, rats were intraperitoneally injected nitroglycerin (10 mg/kg), and 4 h later, blood samples were collected from postcava for measuring the plasma CGRP and β-EP levels. In the reserpine-reduced model test, except the normal control group, mice were administered reserpine (0.25 mg/kg, i.h.) for 9 days. Mice received intragastric administration from the third day to the ninth day. One hour after the last gavage, blood and brain tissue samples were obtained. Then, blood clotting time and the contents of neurotransmitters were determined.ResultsQLTT- (3.6, 1.8, and 0.9 g/kg) and ZTP-treated rats had lower blood viscosity than that in model rats under different shear rates (P< 0.01). QLTT- (3.6, 1.8 g/kg) and ZTP-treated rats had significantly lower plasma CGRP levels and higher plasma β-EP levels than those in model rats (P< 0.01). QLTT treatment at dose of 0.9 g/kg had lower plasma CGRP levels as well (P<0.05). QLTT- (5.2, 2.6 g/kg) and ZTP-treated mice had longer blood clotting time than that in model mice (P<0.01). QLTT- (2.6 g/kg) and ZTP-treated mice had higher plasma serotonin (5-HT) levels than those in model mice (P<0.05).ConclusionQLTT-treated animals had lower plasma CGRP level, higher plasma β-EP, 5-HT, higher brain tissue 5-HT, NE, DA levels, and lower blood viscosity than those in the migraine model animals

Aqua­bis(2-methyl-4-oxopyrido[1,2-a]pyrimidin-9-olato)zinc(II) monohydrate

The crystal structure of the title compound, [Zn(C9H7N2O2)2(H2O)]·H2O, involves discrete mononuclear complex mol­ecules. The special positions on the rotation twofold axis are occupied by ZnII and O atoms of the coordinated and uncoordinated water mol­ecules. The coordination around the ZnII atom can be described as transitional from trigonal-bipyramidal to square-pyramidal. The two chelating 2-methyl-4-oxopyrido[1,2-a]pyrimidin-9-olate ligands and the coordin­ated water mol­ecule form the Zn coordination. O—H⋯O hydrogen bonds between the coordinated water mol­ecule and the ligand and between the uncoordinated water mol­ecule and the ligand dominate the crystal packing