3,020 research outputs found
Poly[[diaquatris[μ4-(p-phenylenedioxy)diacetato]didysprosium(III)] dihydrate]
The title dysprosium coordination polymer, {[Dy2(C10H8O6)3(H2O)2]·2H2O}n, was synthesized by reacting dysprosium(III) nitrate and the flexible ligand (p-phenylenedioxy)diacetic acid under hydrothermal conditions. Each DyIII ion is coordinated by nine O atoms in a tricapped trigonal prismatic geometry. The DyO9 polyhedra form layers parallel to the bc plane. The rigid benzene rings of the anions link the layers along the a axis, forming a three-dimensional framework
Downregulation of KLF8 expression by shRNA induces inhibition of cell proliferation in CAL27 human oral cancer cells
Objectives: KLF8 is a member of KLF transcription factors which play an important tolr in oncogenesis. It is
barely expressed in normal human epithelial cells but highly overexpressed in several types of human cancer cell
lines. In the present study, we investigate the role of KLF8 in oral cancer and the effects of KLF8 knockdown via
lentivirus mediated siRNA infection in human adenosquamos carcinoma CAL 27 cells.
Study
Design:
We developed a vector-based siRNA expression system that can induce RNAi in CAL 27 oral cancer cells. Downregulation of KLF8 was confirmed by evaluating GFP expressions, RT-PCR and western blot analysis. Finally, the effects of KLF8 downregulation were analyzed by MTT assay and colony formation assays.
Results: The expression levels of KLF8 mRNA and proteins are reduced in CAL 27 cells that transfected with 21-
nt siRNA against KLF8. Lentivirus-mediated silencing of KLF8 reduces cell proliferation and colonies number,
thereby indicating the role of KLF8 in cell proliferation and tumorigenesis.
Conclusions: These results strongly suggest that KLF8 is essential for growth of CAL 27 cancer cells. A better understanding of KLF8 function and processing may provide novel insights into the clinical therapy of oral cancer
Increased Number of Tc17 and Correlation with Th17 Cells in Patients with Immune Thrombocytopenia
BACKGROUND: IL-17-secreting CD8+ T cells (Tc17 subset) have recently been defined as a subpopulation of effector T cells implicated in the pathogenesis of autoimmune diseases. The role of Tc17 and correlation with Th17 cells in the pathophysiology of immune thrombocytopenia (ITP) remain unsettled. DESIGN AND METHODS: We studied 47 ITP patients (20 newly-diagnosed and 27 with complete response) and 34 healthy controls. IL-17-producing CD3+CD8+ cells (Tc17) and IL-17-producing CD3+CD8- cells (Th17) were evaluated by flow cytometry and expressed as a percentage of the total number of CD3+ cells. Specific anti-platelet glycoprotein (GP) GPIIb/IIIa and/or GPIb/IX autoantibodies were measured by modified monoclonal antibody specific immobilization of platelet antigens. Peripheral blood mononuclear cells of ITP patients were isolated, incubated in the presence of 0, 0.25, 0.5, or 1 µmol/L of dexamethasone for 72 h, and collected to detect Tc17 and Th17 cells by flow cytometric analysis. RESULTS: IL-17 was expressed on CD3+CD8- and CD3+CD8+ T cells. The percentages of Tc17 and Th17 cells in newly-diagnosed patients were significantly elevated compared to controls, and Tc17 was decreased after clinical treatment. The Th17∶Tc17 ratio was significantly lower in newly-diagnosed patients compared with controls, and was increased in patients who had complete response. There was a significantly positive correlation between Tc17 and Th17 cells in the control group, but not in the ITP patients. A positive correlation existed between Tc17 and the CD8∶CD4 ratio, as well as CD8+ cells in patients with ITP. The frequencies of Tc17 were marginally higher in autoantibody-negative patients than autoantibody-positive patients. Moreover, both Tc17 and Th17 cell percentages decreased as the concentration of dexamethasone in the culture media increased in ITP patients. CONCLUSIONS: Tc17 and the Th17 subset are involved in the immunopathology of ITP. Blocking the abnormally increased number of Tc17 may be a reasonable therapeutic strategy for ITP
Decreased NPC1L1 expression in the liver from Chinese female gallstone patients
<p>Abstract</p> <p>Background</p> <p>Cholesterol gallstone disease is a very common disease in both industrialized and developing countries. Many studies have found that cholesterol gallstones are more common in women than men. The molecular mechanisms underlying the relationship between female gallstone disease and hepatic sterol transporters are still undergoing definition and have not been evaluated in humans.</p> <p>Aims</p> <p>The aim of this study is to probe for underlying hepatic molecular defects associated with development of gallstones in female.</p> <p>Methods/Results</p> <p>Fifty-seven nonobese, normolipidemic Chinese female gallstone patients (GS) were investigated with 12 age- and body mass index-matched female gallstone-free controls (GSF). The bile from the female GS had higher cholesterol saturation than that from the female GSF. The hepatic NPC1L1 mRNA levels were lower in female GS, correlated with SREBP2 mRNA. NPC1L1 downregulation was confirmed at protein levels. Consistently, immunohistochemistry showed decreased NPC1L1 expression in female GS.</p> <p>Conclusions</p> <p>The decreased hepatic NPC1L1 levels in female GS might indicate a downregulated reabsorption of biliary cholesterol in the liver, which, in turn, leads to the cholesterol supersaturation of bile. Our data are consistent with the possibility that hepatic NPC1L1 may be mediated by SREBP2.</p
Analysis on Steady-State Operation and Heat Loss of Chinese Integrated Pressurized Water Reactor
Chinese integrated pressurized water reactor (CIPWR) has compact configuration and high inherent safety, which is appropriate for nuclear power plants of small and medium scale. Heat balance model has been adopted widely in thermal power calibration of PWRs because of its advantage of accuracy. In this paper, a package based on FORTRAN language is developed and added into RELAP5 to calculate the heat loss value needed in heat balance analysis. The steady-state operation of CIPWR is modelled correctly by RELAP5. The heat loss of CIPWR is calculated by the package, and the comparison of the main values of parameters needed in the heat loss calculation between RELAP5 and the package has been done. It shows that the package has high calculation accuracy and can be applied in reactor design and monitoring
Deja Vu: Contextual Sparsity for Efficient LLMs at Inference Time
Large language models (LLMs) with hundreds of billions of parameters have
sparked a new wave of exciting AI applications. However, they are
computationally expensive at inference time. Sparsity is a natural approach to
reduce this cost, but existing methods either require costly retraining, have
to forgo LLM's in-context learning ability, or do not yield wall-clock time
speedup on modern hardware. We hypothesize that contextual sparsity, which are
small, input-dependent sets of attention heads and MLP parameters that yield
approximately the same output as the dense model for a given input, can address
these issues. We show that contextual sparsity exists, that it can be
accurately predicted, and that we can exploit it to speed up LLM inference in
wall-clock time without compromising LLM's quality or in-context learning
ability. Based on these insights, we propose DejaVu, a system that uses a
low-cost algorithm to predict contextual sparsity on the fly given inputs to
each layer, along with an asynchronous and hardware-aware implementation that
speeds up LLM inference. We validate that DejaVu can reduce the inference
latency of OPT-175B by over 2X compared to the state-of-the-art
FasterTransformer, and over 6X compared to the widely used Hugging Face
implementation, without compromising model quality. The code is available at
https://github.com/FMInference/DejaVu
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