13 research outputs found
CADM2, as a new target of miR-10b, promotes tumor metastasis through FAK/AKT pathway in hepatocellular carcinoma
Abstract Background Cell adhesion molecules (CADMs) comprise of a protein family whose functions include maintenance of cell polarity and tumor suppression. Hypo-expression of CADM2 gene expression has been observed in several cancers including hepatocellular carcinoma (HCC). However, the role and mechanisms of CADM2 in HCC remain unclear. Methods The expression of CADM2 and miRNA-10b (miR-10b) in HCC tissues and cell lines were detected using real-time PCR and Western blotting. Immunofluorescence was used to detect Epithelial-mesenchymal transition (EMT) progression in HCC cell lines. Dual-luciferase reporter assay was used to determine miR-10b binding to CADM2 3’UTR. Wound healing assay and Transwell assay were performed to examine the migration and invasion of HCC cells. Results We report the effect of CADM2 as a tumor suppressor in HCC. Firstly, we confirmed that CADM2 expression was significantly down regulated in HCC tissues compared to normal tissues according to TCGA data analysis and fresh HCC sample detection. Secondly, overexpression of CADM2 could inhibit EMT process, migratory and invasion ability of HCC cells. Furthermore, the results indicated that CADM2 is a direct target of miR-10b in HCC cells and miR-10b/CADM2 modulates EMT process and migration ability via focal adhesion kinase (FAK) /AKT signaling pathway in HCC. Conclusions Our study demonstrates that miR-10b-CADM2-FAK/AKT axis plays an important role in HCC metastasis, which might be a novel potential therapeutic option for HCC treatment
Vapor Phase Growth of Air-Stable Hybrid Perovskite FAPbBr<sub>3</sub> Single-Crystalline Nanosheets
Organic–inorganic
hybrid perovskites have attracted
tremendous
attention owing to their fascinating optoelectronic properties. However,
their poor air stability seriously hinders practical applications,
which becomes more serious with thickness down to the nanoscale. Here
we report a one-step vapor phase growth of HC(NH2)2PbBr3 (FAPbBr3) single-crystalline nanosheets
of tunable size up to 50 μm and thickness down to 20 nm. The
FAPbBr3 nanosheets demonstrate high stability for over
months of exposure to air with no degradation in surface roughness
and photoluminescence efficiency. Besides, the FAPbBr3 photodetectors
exhibit superior overall performance as compared to previous devices
based on nonlayered perovskite nanosheets, such as an ultralow dark
current of 24 pA, an ultrahigh responsivity of 1033 A/W, an external
quantum efficiency over 3000%, a rapid response time around 25 ms,
and a high on/off ratio of 104. This work provides a strategy
to tackle the challenges of hybrid perovskites toward integrated optoelectronics
with requirements of nanoscale thickness, high stability, and excellent
performance
Engineering the Phases and Heterostructures of Ultrathin Hybrid Perovskite Nanosheets
Low-dimensional perovskites have gained increasing attention recently, and engineering their material phases, structural patterning and interfacial properties is crucial for future perovskite-based applications. Here a phase and heterostructure engineering on ultrathin perovskites, through the reversible cation exchange of hybrid perovskites and efficient surface functionalization of low-dimensional materials, is demonstrated. Using PbI2 as precursor and template, perovskite nanosheets of varying thickness and hexagonal shape on diverse substrates is obtained. Multiple phases, such as PbI2, MAPbI3 and FAPbI3, can be flexibly designed and transformed as a single nanosheet. A perovskite nanosheet can be patterned using masks made of 2D materials, fabricating lateral heterostructures of perovskite and PbI2. Perovskite-based vertical heterostructures show strong interfacial coupling with 2D materials. As a demonstration, monolayer MoS2/MAPbI3 stacks give a type-II heterojunction. The ability to combine the optically efficient perovskites with versatile 2D materials creates possibilities for new designs and functionalities
Engineering the Phases and Heterostructures of Ultrathin Hybrid Perovskite Nanosheets
Low-dimensional perovskites have gained increasing attention recently, and engineering their material phases, structural patterning and interfacial properties is crucial for future perovskite-based applications. Here a phase and heterostructure engineering on ultrathin perovskites, through the reversible cation exchange of hybrid perovskites and efficient surface functionalization of low-dimensional materials, is demonstrated. Using PbI2 as precursor and template, perovskite nanosheets of varying thickness and hexagonal shape on diverse substrates is obtained. Multiple phases, such as PbI2, MAPbI3 and FAPbI3, can be flexibly designed and transformed as a single nanosheet. A perovskite nanosheet can be patterned using masks made of 2D materials, fabricating lateral heterostructures of perovskite and PbI2. Perovskite-based vertical heterostructures show strong interfacial coupling with 2D materials. As a demonstration, monolayer MoS2/MAPbI3 stacks give a type-II heterojunction. The ability to combine the optically efficient perovskites with versatile 2D materials creates possibilities for new designs and functionalities
Additional file 3: of CADM2, as a new target of miR-10b, promotes tumor metastasis through FAK/AKT pathway in hepatocellular carcinoma
Table S4. Information of antibodies used in this study. (DOCX 18 kb
Additional file 2: of CADM2, as a new target of miR-10b, promotes tumor metastasis through FAK/AKT pathway in hepatocellular carcinoma
Table S3. Primers and RNA oligonucleotides. (DOCX 22 kb
Additional file 6: of CADM2, as a new target of miR-10b, promotes tumor metastasis through FAK/AKT pathway in hepatocellular carcinoma
Table S2. Correlations between clinical features and CADM2 expression in 36 HCC patients. (DOCX 19 kb
Additional file 7: of CADM2, as a new target of miR-10b, promotes tumor metastasis through FAK/AKT pathway in hepatocellular carcinoma
Figure S3. Correlation between CADM2 mRNA and its DNA methylation level in human HCC samples. (JPG 7198 kb