Genetic dissection of the biochemical activities of human DNA repair protein, APE1

Human apurinic/apyrimidinic endonuclease 1 (APE1) is a key DNA repair enzyme involved in both base excision repair (BER) and nucleotide incision repair (NIR) pathways. In the BER pathway, APE1 cleaves DNA at AP sites and 3'-blocking moieties generated by DNA glycosylases. In the NIR pathway, APE1 incises DNA 5' to a number of oxidatively damaged bases. Here we propose to identify and characterize critical amino acids of APE1 involved in either BER and/or NIR functions by using the alignment of the known three-dimensional (or tertiary) structures of Xth family AP endonucleases including the Methanothermobacter thermautotrophicus Mth212, Bacillus subtilis ExoA (1), E. coli Xth and human APE1 proteins (2)

Genetic dissection of the biochemical activities of human DNA repair protein, APE1

Human apurinic/apyrimidinic endonuclease 1 (APE1) is a key DNA repair enzyme involved in both base excision repair (BER) and nucleotide incision repair (NIR) pathways. In the BER pathway, APE1 cleaves DNA at AP sites and 3'-blocking moieties generated by DNA glycosylases. In the NIR pathway, APE1 incises DNA 5' to a number of oxidatively damaged bases. Here we propose to identify and characterize critical amino acids of APE1 involved in either BER and/or NIR functions by using the alignment of the known three-dimensional (or tertiary) structures of Xth family AP endonucleases including the Methanothermobacter thermautotrophicus Mth212, Bacillus subtilis ExoA (1), E. coli Xth and human APE1 proteins (2)

E-COMMERCE EVALUATION AND E BUSINESS TRENDS

E-Commerce in recent times has exploded many folds due to the invention of new Information and Communication (ICT) technologies and applications. Business models of today are highly depending upon internet based transaction and trading platforms, use of Web portals and mobile applications. Proposition of new business models sans the inclusion of internet and their application has become almost impossible for business. Business data pertaining to E Commerce has become highly valuable in recent times. Social networking websites, sharing of product data and its reviews in blogs paves ways for promoting business in new methods. There is need to develop metrics to assess the strength of E Commerce penetration in Business and also evaluate the various E Commerce platforms. Use of E business tools for data analysis, prediction and decision making has become the latest order of the day. Changing trends in E business has a drastic impact on the organizations depending upon internet based applications for handling its business processes and day to day transactions.E-Commerce in recent times has exploded many folds due to the invention of new Information and Communication (ICT) technologies and applications. Business models of today are highly depending upon internet based transaction and trading platforms, use of Web portals and mobile applications. Proposition of new business models sans the inclusion of internet and their application has become almost impossible for business. Business data pertaining to E Commerce has become highly valuable in recent times. Social networking websites, sharing of product data and its reviews in blogs paves ways for promoting business in new methods. There is need to develop metrics to assess the strength of E Commerce penetration in Business and also evaluate the various E Commerce platforms. Use of E business tools for data analysis, prediction and decision making has become the latest order of the day. Changing trends in E business has a drastic impact on the organizations depending upon internet based applications for handling its business processes and day to day transactions

The research and analysis of the physico-chemical composition of irga and chokeberry

Recently, there has been a need to conduct research on obtaining food additives based on natural raw materials, finding optimal methods of its processing, creating waste-free technologies, as well as expanding the production of food enriched with vitamins, protein and other components of increased nutritional value. It is known that about 500 species of wild raw materials grow in Northern Kazakhstan, which is a potential source of biologically active components. Many of these plants are not used in the food industry. The significance of the chemical components of fruits, vegetables and berries varies, but they are all necessary for a rational human nutrition. The demand for berries and other derivatives of their products has increased with the growth of global awareness about the role of these and other types of fresh fruits/foods, which has aroused increased interest in the scientific community not only in the study of their individual potential, but also in the development of functional foods enriched with berries. This paper presents a brief analytical review, organoleptic and chemical composition of the berries of irga and chokeberry. The influence of heat treatment on the chemical composition of juices has been determined. The optimal drying modes of the pomace of irgi berries and chokeberry have been investigated. The possibility and relevance of using these berries and squeezing berries as dietary supplements is substantiated. The study of the chemical composition of the berries of chokeberry and irga and its further use as dietary supplements in the food industry will help to create new enriched healthy foods for the population

Improving the technology of whey-based cheeses

The article provides the main qualities and characteristics of whey, the results of obtaining whey-based cheeses. All the properties of the serum have been determined, and the formulations of the new product have also been developed. Whey cheese formulations based on different types of whey have also been developed

Space-time continuum in A. Blok’s cycle «Poems about the Beautiful Lady»

Temirgazina, Z. K. Space-time continuum in A. Blok’s cycle «Poems about the Beautiful Lady» / Z. K. Temirgazina, S. V. Nikolayenko, G. K. Zhakupova // Вестник Карагандинского университета. Серия «Филология». – 2020. – № 1 (97). – С. 71–77.The article deals with a problem about the space-time continuum on the material of the cycle of poems by A. Blok «Poems about the Beautiful Lady». The analysis of poems is presented, semiotic dominants in the space-time category are revealed. The novelty of the article lies in the fact that the space-time continuum of this cycle of poems has not been studied from the point of view of semiotics. As a result of the work it was revealed that space and time form an indissoluble unity in the cycle. The lyrical hero of Blok in most poems about a Beautiful lady occupies a position between two worlds, on the edge, on the border between this world and the other world. At the same time, in a cycle it is possible to trace finding of the Beautiful Lady in «horizontal » and «vertical» spaces. The next direction in the analysis of space-time relations was the differentiation of linear and cyclic time. All locations and stay of a Beautiful Lady are endowed with respectively positive and negative characteristics. The lyrical hero is as it were between the visible and the invisible, the revealed and the hidden, the manifest and the secret, the real and the unreal. Thus, as a result of the study, we came to the conclusion that the cycle of poems is characterized by the awareness of space and time through the prism of the experience accumulated by the lyrical hero, the expectation of a Beautiful Lady