Secretory immune system of saliva in irritable bowel syndrome on children

Background. It is known there are links between irritable bowel syndrome and development of allergy, between irritable bowel syndrome and hypoergical immunological reactions. On the other side, there is link between irritable bowel syndrome and non adequate activation of the mucosal immune system as the result of cytokin s dysbalance. That is why we investigated the secretory immune system of saliva in children with irritable bowel syndrome. The purpose of this study was to determine secretory immune system of saliva on children with different forms of irritable bowel syndrome. Methods. 102 children with irritable bowel syndrome were examined. The level of the immunoglobulins (slgA, IgA, IgG, IgM, IgE, lgG1-4), the concentration of TN F-a and lactoferrin, the total activity of the complement system (CH50) and its components (C1-C5) were obtained in the saliva. Results. It was revealed secretory immune system of saliva in children with irritable bowel syndrome was significantly differed from its of healthy children. The level of IgA, IgM in the saliva of children with irritable bowel syndrome was less than in healthy children, however, the level of IgG t-4, IgE in the saliva was significantly higher. The prevalence of the food allergy (atopy history and clinical symptoms) in children with irritable bowel syndrome was 42.2% and was differed from prevalence of the food allergy in population. Perhaps, this fact confirms the IgE-depending pathway of the irritable bowel syndrome. The saliva s level of the total activity of the complement system (CH50) and its components (C1-C5) and the concentration of TN F-a was decreased on children with irritable bowel syndrome. Also it was determined the increasing of the saliva s level of slgA, IgA, IgG, IgM, lgG1-4 was typical for the irritable bowel syndrome with abdominal pain and meteorism. The levels of some of these immune proteins in irritable bowel syndrome with diarrhea or obstipation were decreased. Conclusion Thus, these peculiarities of the secretory immunity of saliva in irritable bowel syndrome are dysregulating troubles. This fact confirms mucosal immune system takes part in development of the irritable bowel syndrome.В работе изучается характер изменений показателей секреторного иммунитета слюны у детей с синдромом раздраженного кишечника (СРК) в связи с предполагаемой связью СРК с формированием аллергии, с наличием гипзргических иммунологических реакций и напротив, с неадекватной активацией мукозальной иммунной системы в результате нарушения цитокинергической регуляции. Для проведения исследования секреторного иммунитета слюны обследовано 102 ребенка с СРК. В слюне определялось: количество иммуноглобулинов А, М. G, подклассы lg G1-4, количество slgA и IgE, активность СН50 и компонентов комплемента С1-С5, количество лактоферрина и туморнекротичский фактор TNF-a. Показатели секреторного иммунитета слюны у детей с синдромом раздраженного кишечника значительно отличаются от параметров здоровых детей. В общей группе больных детей с СРК отмечается снижение иммуноглобулинов lg A, lg М, тогда как количество IgG. Субклассов lg G1-4, IgE значительно и достоверно повышено. Происходит снижение активности отдельных компонентов комплемента и TNF-a. т.е. флогогенных факторов. Изменения показателей секреторного иммунитета слюны при синдроме раздраженного кишечника относятся к дизрегуляторным нарушениям и могут свидетельствовать об участии мукозального иммунитета в формировании данной дисфункции кишечника

Clinical application of induced sputum in children with newly diagnosed asthma: cellular and immunologic characteristics

Authors investigate cells and immunologic factors of induced sputum in children with newly diagnosed asthma and healthy children without atopy. The aim of the study was to find out the differences of cellular and immunologic profiles of induced sputum in children with newly diagnosed asthma. 35 children aged 1,5-5 years old (Me = 3,5 years) were include in this study: 18 children with newly diagnosed asthma and 17 children (control group) without allergic diseases, which had no respiratory symptoms during last month. Sputum induction carried out according to our modification of protocol developed by Pin et al. The levels of IgE, slgA, lgG4, IL-1β, IL-4, IL-8, IL-13, TNFα, INFγ, N03, NOX and cells percentage (macrophages, neutrophils, eosinophils, lymphocytes) were evaluated in sputum. Results. The percentage of eosinophils was significantly higher and the percentage of macrophages was significantly lower in induced sputum of children with newly diagnosed asthma. The levels of proinflammatory factors (IL-1β, IL-4, IL-8, IL-13, TNFα), immunoglobulins, which participate in allergic inflammation (IgE, slgA, lgG4) and stable metabolites of NO (NO3, NOX) in sputum were also significantly higher in children with newly diagnosed asthma.Проводился подсчет клеток и определение иммунологических факторов в индуцированной мокроте у детей с вперсые выявленной бронхиальной астмой и здоровых детей без атопии. Целью исследования было выявление цитоиммунологических особенностей индуцированной мокроты у детей раннего возраста с впервые выявленной бронхиальной астмой. В исследовании приняли участие 35 детей в возрасте 1,5-5 лет (средний возраст 3,5г.): 18 детей с впервые выявленной бронхиальной астмой и 17 практически здоровых детей без атопии - контрольная группа. У всех детей в исследовании в течение предшествующего месяца не было зарегистрировано эпизодов респираторной инфекции. Индукция мокроты проводилась по модифицированному нами протоколу с использованием гипертонического раствора хлорида натрия. Прототипом явился метод, разработанный Pin et al., исследовался клеточный состав (%) (макрофаги, нейтрофилы, эозинофилы, лимфоциты) и иммунологический профиль (IgE, slgA, lgG4, IL-1β, IL-4, IL-8, IL-13. TNFα, INFγ, NO3, NOX) индуцированной мокроты. Результаты. У детей с впервые выявленной бронхиальной астмой в индуцированной мокроте уровень эозинофилов (%) достоверно выше, а уровень макрофагов (%) достоверно ниже по сравнению со здоровыми детьми без атопии. Так же у детей с бронхиальной астмой выявлены более высокие концентрации провоспалительных цитокинов (ФНОа, ИЛ 4, IL-1B, ИЛ8, ИЛ 13), иммуноглобулинов (IgE, lgG4) участвующих в аллергическом воспалении бронхов при бронхиальной астме и конечных стабильных метаболитов оксида азота (NO3, NOX) по сравнению с показателями здоровых детей без атопии

Application of NMR for quantification of magnetic nanoparticles and development of paper-based assay

H1 NMR relaxometry is a method that is extremely sensitive to the presence of magnetic nanoparticles, which significantly affect the transverse relaxation time of the water proton. Accordingly, the use of magnetic nanoparticles as labels allows detection of even extremely small amounts of the test substance. This paper analyzes the prospects for applying the method of solid-phase NMR-relaxometric determination of biologically active molecules. The nitrocellulose membranes are chosen as a solid phase and nanoparticles based on iron core with a carbon shell are used as magnetic labels. The possibility of detecting small concentrations of magnetic particles in porous medium is demonstrated. Finally, the ability to detect extremely low concentrations of an analyte, in this case, streptavidin protein (0.5 ng/ml to 100 ng/ml), which is actively used in various fields of biology and medicine, is demonstrated. © Published under licence by IOP Publishing Ltd.Russian Science Foundation, RSF: 17-15-01116The work was carried out within the Russian Science Foundation project 17-15-01116. equipment of the Ural Center for Shared Use Modern nanotechnology UrFU was used

The problem of predisposition to diseases: classic approaches and modern technologies

The article is devoted to urgent pediatric problem of comparing traditional and innovative approaches to the study of predisposition to diseases. It is noted that in the era of the formation of personalized pediatrics, along with the introduction of the latest diagnostic and therapeutic technologies, it is important to take into account the classic achievements, including those associated with the ideology of diathesis developed in the Russian pediatrics – concepts of hereditary predispositions. The article contains data on changing classification of diathesis. Within the framework of the 4P medical paradigm and, first of all, personalized medicine, the authors discuss molecular genetic and other high-tech potential methods of the disease risk assessment. Based on the concept of the space-time bioecological continuum of transitional health states, in particular, using the example of the atopic march, the authors consider the approaches to multi-level research, adaptation mechanisms and their breakdowns, and possible development of appropriate prognostic biomarkers. Using the example of biophenotypes of bronchial asthma and variants of manifestations of disenergetic states, the authors pay their attention to the importance of identifying the individual characteristics of pathological processes in children. The authors have concluded that the main task of personalized pediatrics is the development of methodology used for designing a personal health management program based on the principles of a new strategy for diagnosing, monitoring and controlling individual (including genomic) health, with the formation of a genetic passports for each child

Environmental Changes of the Aral Sea (Central Asia) in the Holocene: Major Trends

From the 20th International Radiocarbon Conference held in Kona, Hawaii, USA, May 31-June 3, 2009.Changes of the Aral Sea level have been observed in 3 sediment boreholes, 2 outcrops, and associated archaeological sites. The obtained results are supported by 25 radiocarbon dates. Major trends of lake-level changes have been reconstructed in some detail for the last 2000 yr, and additional data provide an outline of fluctuations throughout the Holocene. Several distinct changes are shown to precede the modern, human-induced regression of the Aral Sea. These include: 1) the latest maximum in the 16th-20th centuries AD (53 m asl); 2) a Medieval "Kerderi" minimum of the 12th-15th centuries AD (29 m asl); 3) the early Medieval maximum of the 4th-11th centuries AD (52 m asl); and 4) a near BC/AD lowstand, whose level is not well established. Since then, events are only inferred from sparse data. The studied cores contain several sandy layers representing the lowering of the lake level within the Holocene, including the buried shore-bar of ~4500 cal BP (38 m asl), and shallow-water sediments of ~5600 cal BP (44 m asl), 7200 cal BP (28 m asl), and 8000 cal BP (26.5 m asl).The Radiocarbon archives are made available by Radiocarbon and the University of Arizona Libraries. Contact [email protected] for further information.Migrated from OJS platform February 202