The role of immunohistochemistry in distinguishing malignant mesothelioma and adenocarcinoma

WOS: 000249454601271

Pulmonary effects of deltamethrin inhalation: an experimental study in rats

PubMedID: 16677916Deltamethrin is a synthetic pyrethroid widely used as the insecticide of choice especially for local vector mosquitoes in most countries. The application is mostly by cold aerosol spraying using vehicle-mounted equipment with a duration of 3-4 months during the summer. This experimental study aimed to evaluate the morphologic changes in the lungs caused by the inhalation of this insecticide. The study was performed on 30 mature male Wistar rats. While 10 of the rats were used as control group, 20 rats, separated into two groups, were exposed to 1:10 dilution of deltamethrin aerosol spray for 30 min each day for 45 days in doses of 6.0 and 12.0 mg/m3. The animals were sacrificed and tissue samples taken from the lungs were processed for both light microscopy and transmission electron microscopy. Light microscopic examination revealed heavy congestion, marked perivascular edema, and lymphoplasmocytic infiltration with focal nonspecific interstitial pneumonia, foamy macrophage accumulation, emphysema, peribronchial lymphoid tissue hyperplasia, and focal hemorrhage. Ultrastructurally, the ciliated cells of the airways appeared swollen with a few structurally abnormal cilia. Alveolar lining cells revealed mild degeneration and a slight hyperplasia in type II cells. Increases in the number of collagen bundles and edema in the alveolar septa were also noted. © 2004 Elsevier Inc. All rights reserved.This study was supported by the Cukurova University Research Foundation

Light chain deposition disease with typical morphologic and ultrastructural findings

Light chain deposition disease (LCDD), a rare form of monoclonal gammopathy, is characterized by deposits of amorphous light-chain material mainly in the kidneys and usually associated with Multiple Myeloma (MM). A patient with LCDD having typical morphological, immunohistochemical and ultrastructural changes in kidney biopsy was diagnosed and presented here. We described a 55 year-old woman who had presented with generalized edema, malaise, nausea and vomiting. Laboratory examination revealed anemia, hypergammaglobulinemia, hypoalbuminemia, Bence-Jones proteinuria. Diagnosis of MM was established on the basis of laboratory features, bone marrow aspiration (material was insufficient but had 15-20% plasma cells) and trephine bone marrow biopsy (plasma cell infiltration with moderate reticulin). Renal biopsy disclosed prominent nodular expansion of the mesangium. Ultrastructurally granular electron dense deposits were observed along the glomerular and tubular basement membrane and mesangium typical of LCDD. An immunohistochemical study showed depositions of kappa light chain in the nodules and tubular basement membrane. Patient was accepted as LCDD and treated with 6 course of VAD (including vincristine, doxorubicine, dexamethasone). Partial remission was achieved and high dose chemotherapy and stem cell transplantation were planned.Light chain deposition disease (LCDD), a rare form of monoclonal gammopathy, is characterized by deposits of amorphous light-chain material mainly in the kidneys and usually associated with Multiple Myeloma (MM). A patient with LCDD having typical morphological, immunohistochemical and ultrastructural changes in kidney biopsy was diagnosed and presented here. We described a 55 year-old woman who had presented with generalized edema, malaise, nausea and vomiting. Laboratory examination revealed anemia, hypergammaglobulinemia, hypoalbuminemia, Bence-Jones proteinuria. Diagnosis of MM was established on the basis of laboratory features, bone marrow aspiration (material was insufficient but had 15-20% plasma cells) and trephine bone marrow biopsy (plasma cell infiltration with moderate reticulin). Renal biopsy disclosed prominent nodular expansion of the mesangium. Ultrastructurally granular electron dense deposits were observed along the glomerular and tubular basement membrane and mesangium typical of LCDD. An immunohistochemical study showed depositions of kappa light chain in the nodules and tubular basement membrane. Patient was accepted as LCDD and treated with 6 course of VAD (including vincristine, doxorubicine, dexamethasone). Partial remission was achieved and high dose chemotherapy and stem cell transplantation were planned

Expression of survivin and topoisomerase II alpha in ovarian carcinoma: Correlation with clinicopathological parameters and prognosis

WOS: 000249454600886

A molecular study on selected BRCA-1 and BRCA-2 germline mutations in breast, ovary and fallopian tube cancer cases in Turkey

WOS: 000249454601204

Causes of pulmonary granulomas: a retrospective study of 500 cases from seven countries

Background The frequencies of various causes of pulmonary granulomas in pathological material are unknown, as is the influence of geographical location on aetiology. The aim of this study was to identify the causes of pulmonary granulomas in pathological specimens, to define their frequencies, and to determine whether these causes vary by geographical location. Methods 500 lung biopsies and resections containing granulomas were reviewed retrospectively by expert pulmonary pathologists from 10 institutions in seven countries. Fifty consecutive cases from each location were assigned a diagnosis based on histological features and available clinical/microbiological data. Results A specific cause was identified in 58% of cases (290/500), most commonly sarcoidosis (136, 27%) and mycobacterial or fungal infections (125, 25%). Mycobacteria were identified in 19% of cases outside the USA versus 8% within the USA. In contrast, fungi accounted for 19% cases in the USA versus 4% in other locations. Fungi were mostly detected by histology, whereas most mycobacteria were identified in cultures. In 42% of cases (210/500) an aetiology could not be determined. Conclusions Across several geographical settings, sarcoidosis and infections are the most common causes of pulmonary granulomas diagnosed in pathological specimens. Fungi are more commonly identified than mycobacteria in the USA, whereas the reverse is true in other countries. A definite aetiology cannot be demonstrated in more than a third of all cases of pulmonary granulomas, even after histological examination. These findings highlight the need to submit material for histology as well as cultures in all cases in which granulomatous disease enters the differential diagnosis