A 53-year-old male who underwent 1-level corpectomy with a titanium mesh cage used for cervical reconstruction.

<p>The preoperative cervical X-ray film (4A) and immediately postoperative lateral X-ray (4B) show C5 corpectomy and the titanium mesh cage used for reconstruction. The lateral X-ray one year postoperative (4C) and at the final follow-up (two years and six months; 4D) shows bony fusion between the graft and the adjacent endplates; nevertheless, marked cage subsidence was observed.</p

A 61-year-old male who underwent 2-level corpectomy with a nano-hydroxyapatite/polyamide66 cage used for cervical reconstruction.

<p>A preoperative cervical X-ray film (3A) shows a loss of cervical lordosis. The immediately postoperative lateral X-ray (3B) shows C5 and C6 corpectomy and the n-HA/PA66 cage used for reconstruction. The 3D-CT (3C) and lateral X-ray (3D) show obvious bony fusion and restoration of cervical alignment at the final follow-up (four years and four months).</p

Photographs of lateral (1A) and superior (1B) views of the nano-hydroxyapatite/polyamide66 cage.

<p>Photographs of lateral (1A) and superior (1B) views of the nano-hydroxyapatite/polyamide66 cage.</p

A 36-year-old male who underwent 1-level corpectomy with a nano-hydroxyapatite/polyamide66 cage used for cervical reconstruction.

<p>The preoperative cervical X-ray film (2A) and MRI scan (2B) show the spinal cord compression resulting from C4/5 and C5/6 disc herniations. The immediately postoperative lateral X-ray (2C) shows C5 corpectomy and the n-HA/PA66 cage used for reconstruction, and an obvious radiolucent gap can be observed between the cage and the endplates. The lateral X-ray film (2D) shows no obvious radiolucent gap, and the 3D-CT (2E) scan shows the autogenous bone granules filling the cage and achieving bony fusion with adjacent endplates at the 1-year follow-up. A lateral X-ray film (2F) at the final follow-up (four years and eight months) shows satisfying bony fusion and no obvious migration or subsidence.</p

Demographic and clinical data of patients.

<p>Demographic and clinical data of patients.</p

Radiographic and clinical outcomes in each group.

<p>Radiographic and clinical outcomes in each group.</p

A 46-year-old male who underwent 2-level corpectomy with a titanium mesh cage used for cervical reconstruction.

<p>A cervical MRI scan (5A) shows multi-level disc herniations (C4/5, C5/6, C6/7) and oppression of the spinal cord. The immediately postoperative lateral X-ray (5B) shows C5 and C6 corpectomy and the titanium mesh cage used for reconstruction in which the cervical alignment was marginally restored. A lateral X-ray at the one-year follow-up (5C) shows subsidence. A lateral X-ray at the final follow-up (5D) shows increased subsidence and a loss of cervical alignment.</p

Data_Sheet_1_Development and validation of a novel necroptosis-related gene signature for predicting prognosis and therapeutic response in Ewing sarcoma.zip

Ewing sarcoma (ES) is the second most common malignant bone tumor in children and has a poor prognosis due to early metastasis and easy recurrence. Necroptosis is a newly discovered cell death method, and its critical role in tumor immunity and therapy has attracted widespread attention. Thus, the emergence of necroptosis may provide bright prospects for the treatment of ES and deserves our further study. Here, based on the random forest algorithm, we identified 6 key necroptosis-related genes (NRGs) and used them to construct an NRG signature with excellent predictive performance. Subsequent analysis showed that NRGs were closely associated with ES tumor immunity, and the signature was also good at predicting immunotherapy and chemotherapy response. Next, a comprehensive analysis of key genes showed that RIPK1, JAK1, and CHMP7 were potential therapeutic targets. The Cancer Dependency Map (DepMap) results showed that CHMP7 is associated with ES cell growth, and the Gene Set Cancer Analysis (GSCALite) results revealed that the JAK1 mutation frequency was the highest. The expression of 3 genes was all negatively correlated with methylation and positively with copy number variation (CNV). Finally, an accurate nomogram was constructed with this signature and clinical traits. In short, this study constructed an accurate prognostic signature and identified 3 novel therapeutic targets against ES.</p