The stiffness of elastomeric surfaces influences the mechanical properties of endothelial cells

Optimal characterization of the mechanical properties of both cells and their surrounding is an issue of major interest. Indeed, cell function and development are strongly influenced by external stimuli. Furthermore, a change in cell mechanics might, in some cases, associate with diseases or malfunctioning. In this work, atomic force microscopy (AFM) was applied to examine the mechanical properties of the silicone elastomer polydimethylsiloxane (PDMS) a common substrate in cell culture. Force spectroscopy analysis was done over different specimens of this elastomeric material containing varying ratios of resin to cross-linker in its structure (5:1, 10:1, 20:1, 30:1 and 50:1), which impacts the final material properties (e.g., stiffness, elasticity). To quantify the mechanical properties of the PDMS, factors as the modulus of Young, the maximum adhesive forces as well as both relaxation amplitudes and times upon constant height contact of the tip (dwell time different of zero) were calculated from the different segments forming the force curves. It is demonstrated that the material stiffness is increased by prior oxygen plasma treatment of the sample, required for hydrophilic switching, contrarily to what observed for its adhesiveness. Subsequent incubation of endothelial HUVEC cells on top of these plasma treated PDMS systems yields minor variation in cell mechanics in comparison to those obtained on a glass reference, on which cells show much higher spreading tendency and, by extension, a remarkable membrane hardening. Thus, surface wettability turns a factor of higher relevance than substrate stiffness inducing variations in the cell mechanics.Comment: manuscript (12 pages, 4 figures, 2 tables), supplementary information (2 pages and 3 figures), the main results of the manuscript are based on a master thesi

Comparison of cauliflower-insect-fungus interactions and pesticides for cabbage root fly control

International audienceCabbage root fly (Delia radicum L.) control represents a major challenge in brassica production, therefore different management strategies for its control were tested in conventionally managed open field cauliflower production. Strategies included treatments with low-risk methods such as nitrogen lime, the insecticide spinosad and the Beauveria bassiana ATCC 74040-based biopesticide Naturalis. Their effects were compared with treatments based on nonformulated fungal species Metarhizium brunneum, B. bassiana, Clonostachys solani, Trichoderma atroviride, T. koningiopsis, and T. gamsii and commercial insecticides.-cyhalothrin and thiamethoxam. Spinosad and thiamethoxam were pipetted to individual plants before transplanting; lambda-cyhalothrin was sprayed after transplanting; nitrogen lime was applied at first hoeing. Nonformulated fungi were delivered onto cauliflower plantlets' roots as a single pretransplantation inoculation. The cabbage root fly population dynamics exhibited a strong spatiotemporal variation. The lowest number of cabbage root fly pupae recovered from cauliflower roots in the field experiments was recorded in plants treated with spinosad (significant reduction), followed by Naturalis and one of the tested M. brunneum strains (nonsignificant reduction). Significantly more pupae were counted in the nitrogen lime treatment. The field experiments showed that a single drench of cauliflower plantlets with spinosad offered consistent and enduring cabbage root fly control. Naturalis and nonformulated fungal isolates did not decrease cabbage root fly pressure significantly, apparently due to lack of statistical power. The implications of the substantial intra-and inter-annual pest pressure variation and the benefits of using single plant treatments are discussed, and recommendations for improvement of rhizosphere-competence utilizing biological control strategies provided