9 research outputs found
Functional assays of miR-135b inhibition and overexpression.
<p>A) Baseline mRNA miR-135b expression in 3 different OTR-derived cSCC cell lines was established by qRT-PCR. B) Effect of miR-135b inhibition on LZTS1 mRNA expression in cSCC lines by qRT-PCR within 48 hours post-transfection. C) Effect of miR-135b overexpression on LZTS1 mRNA expression in cSCC lines by qRT-PCR within 48 hours post-transfection. * indicate p values less than 0.005.</p
miR-135b overexpression increases cell migration and invasion in MET1, PM1 and MET4 cSCC cell lines.
<p>A, B) miR-135b mimic significantly increases cSCC cell migration in the wound scratch assay within 24 hours post-transfection. C, D) miR-135b mimic significantly increases cSCC cell invasion in the Matrigel transwell invasion assay in PM1 cell line. * indicate p values less than 0.01. A) <i>Bar = 100um</i>; B) <i>Bar = 200um</i>.</p
Statistical analysis of differentially expressed miRNAs in cSCC tissues from organ transplant recipients.
<p>Statistical analysis of differentially expressed miRNAs in cSCC tissues from organ transplant recipients.</p
Immunohistochemical analysis of LZTS1 protein levels in cells transfected with miR-135b inhibitor.
<p>A) cSCC cell lines transfected with miR-135b inhibitor showed increased LZTS1staining intensity 48 hours post-transfection when compared to negative control. B) Mean fluorescent intensity per cell was quantified using NIH image J program in cell transfected with miR-135b inhibitor and negative control oligonucleotiides. * indicates p values less than 0.001. <i>Bar = 1000um</i>.</p
miR-135b does not affect the cSCC cell proliferation <i>in vitro</i>.
<p>Proliferation of cSCC cells transfected with miR-135b inhibitor <i>versus</i> negative control oligonucleotides. Each data point was obtained as an average of triplicate samples. P value from two-sided Student's <i>t</i> test compares the measurement of cellular DNA content via fluorescent dye binding between the two groups. p value greater than 0.05 was calculated for all three cell lines at all individual time points.</p
Immunohistochemical analysis of LZTS1 protein levels in cells transfected with miR-135b mimic.
<p>A) All cSCC cell lines transfected with miR-135b mimic showed decreased LZTS1staining intensity 48 hours post-transfection when compared to negative control. B) Mean fluorescent intensity per cell was quantified using NIH image J program in cell transfected with miR-135b mimic and negative control oligonucleotiides. * indicates p values less than 0.001. <i>Bar = 1000um</i>.</p
Immunohistochemical analysis of LZTS1 protein levels in normal human skin and cSCC tissues.
<p>A, C) LZTS1 staining is localized in the normal epidermis, but is largely absent in the tumor tissue. Arrows point towards the tumor nests. The absence of staining with control rabbit IgG antibody confirms the specificity of LZTS1 antibody. B, D) Evaluation of LZTS1 staining intensity shows significant reduction of LZTS1 staining in the tumor tissue when compared to normal non-lesional epidermis. * indicate p values less than 0.001. <i>Bar = 100um</i>.</p
Statistical analysis of differentially expressed miRNAs in cSCC tissues from immunocompetent patients.
<p>Statistical analysis of differentially expressed miRNAs in cSCC tissues from immunocompetent patients.</p
Differentially expressed miRNAs in human cSCC tissue.
<p>A) PCR array analysis of miRNA expression in cSCC tumors from immunocompetent patients (n = 32). B) PCR array analysis miRNA expression in cSCC tumors from organ transplant recipients (n = 11).</p