Glucose and Pharmacological Modulators of ATP-Sensitive K+ Channels Control [Ca2+]c by Different Mechanisms in Isolated Mouse α-Cells

OBJECTIVE—We studied how glucose and ATP-sensitive K+ (KATP) channel modulators affect α-cell [Ca2+]c

[Ca2+]c in single mouse α-cells is controlled by modulators of K+ATP channels but not by glucose

BACKGROUND AND AIMS: Stimulus-secretion coupling in pancreatic α-cells is poorly understood. Changes in the free cytosolic Ca2+ concentration ([Ca2+]c) are implicated in the control of glucagon release, but the mechanisms by which secretagogues modify α-cell [Ca2+]c remain highly controversial. These were studied here in single α-cells isolated from a mouse model expressing a fluorescent protein (FP) under the control of the glucagon promoter. [...