Fatigue Life Prediction of Welded Box Structures

The objective of this study is to predict fatigue life of metal welded boxes. Experimental results of fatigue life are satisfactory predicted using an analytical scheme based on the volumetric approach.Проведено прогнозирование усталостной долговечности сварных коробчатых конструкций. Результаты расчета усталостной долговечности с использованием аналитической схемы, базирующейся на объемном методе, хорошо согласуются с соответствующими экспериментальными данными.Проведено прогнозування довговічності зварних коробчастих конструкцій від утомленості. Результати розрахунку довговічності від утомленості з використанням аналітичної схеми, що базується на об’ємному методі, добре узгоджуються з відповідними експериментальними даними

Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue

Pediatric liver transplantation is often required as a consequence of biliary disorders because of the lack of alternative treatments for repairing or replacing damaged bile ducts. To address the lack of availability of pediatric livers suitable for transplantation, we developed a protocol for generating bioengineered biliary tissue suitable for biliary reconstruction. Our platform allows the derivation of cholangiocyte organoids (COs) expressing key biliary markers and retaining functions of primary extra- or intrahepatic duct cholangiocytes within 2 weeks of isolation. COs are subsequently seeded on polyglycolic acid (PGA) scaffolds or densified collagen constructs for 4 weeks to generate bioengineered tissue retaining biliary characteristics. Expertise in organoid culture and tissue engineering is desirable for optimal results. COs correspond to mature functional cholangiocytes, differentiating our method from alternative organoid systems currently available that propagate adult stem cells. Consequently, COs provide a unique platform for studies in biliary physiology and pathophysiology, and the resulting bioengineered tissue has broad applications for regenerative medicine and cholangiopathies