5 research outputs found
Chemical and biological characterization of lipopolysaccharides from the Pseudomonas syringae pv. maculicola IMV 381 collection culture and its dissociants
Structure of the core oligosaccharide of a rough-type lipopolysaccharide of Pseudomonas syringae pv. phaseolicola
Structure and gene cluster of the O antigen of E. Coli L-19, a candidate for a new O-serogroup
Escherichia coli L-19 isolated from a healthy individual did not agglutinate with any of 21
polyvalent antisera that cover 174 E. coli O-serogroups. The strain was studied in respect to the
O-antigen (O-specific polysaccharide, OPS) structure and genetics. The LPS was isolated by
phenol–water extraction of bacterial cells and cleaved by mild acid hydrolysis to yield the OPS.
The OPS was studied by sugar and methylation analyses, along with 1D and 2D 1
H and 13C NMR
spectroscopy. The established structure of the linear tetrasaccharide repeating unit was found to
be unique among known bacterial polysaccharide structures. A peculiar component of the L-19
OPS was an amide of glucuronic acid with 2-amino-1,3-propanediol (2-amino-2-deoxyglycerol)
(GroN). The O-antigen gene cluster of L-19 between the conserved genes galF and gnd was
sequenced, and gene functions were tentatively assigned by a comparison with sequences in the
available databases and found to be in agreement with the OPS structure. Except for putative
genes for synthesis and transfer of GroN, the sequences in the L-19 O-antigen gene cluster were
little related to those of reference strains of the 174 known E. coli O-serogroups. The data
obtained suggest that L-19 can be considered as a candidate for a new E. coli O-serogroup
Structure and gene cluster of the O antigen of E. Coli L-19, a candidate for a new O-serogroup
Escherichia coli L-19 isolated from a healthy individual did not agglutinate with any of 21
polyvalent antisera that cover 174 E. coli O-serogroups. The strain was studied in respect to the
O-antigen (O-specific polysaccharide, OPS) structure and genetics. The LPS was isolated by
phenol–water extraction of bacterial cells and cleaved by mild acid hydrolysis to yield the OPS.
The OPS was studied by sugar and methylation analyses, along with 1D and 2D 1
H and 13C NMR
spectroscopy. The established structure of the linear tetrasaccharide repeating unit was found to
be unique among known bacterial polysaccharide structures. A peculiar component of the L-19
OPS was an amide of glucuronic acid with 2-amino-1,3-propanediol (2-amino-2-deoxyglycerol)
(GroN). The O-antigen gene cluster of L-19 between the conserved genes galF and gnd was
sequenced, and gene functions were tentatively assigned by a comparison with sequences in the
available databases and found to be in agreement with the OPS structure. Except for putative
genes for synthesis and transfer of GroN, the sequences in the L-19 O-antigen gene cluster were
little related to those of reference strains of the 174 known E. coli O-serogroups. The data
obtained suggest that L-19 can be considered as a candidate for a new E. coli O-serogroup