Inhibition of AGS cancer cell proliferation following siRNA-mediated downregulation of VEGFR2

Vascular endothelial growth factor (VEGF) and VEGF receptors (VEGFRs) play important roles in angiogenesis of different developmental mechanisms such as wound healing, embryogenesis and diseases, including different types of cancer. VEGFR2 is associated with cell proliferation, migration, and vascular permeability of endothelial cells. Blocking VEGF and its receptors is suggested as a therapeutic approach to prevent tumor growth. In this study, we aim to block VEGF signaling via small interfering RNA (siRNA) inhibition of VEGFR2. Materials and Methods: In this experimental study, we used the RNA interference (RNAi) mechanism to suppress expression of the VEGFR2 gene. We conducted the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay, real-time polymerase chain reaction (PCR), Western blot, and?ow cytometry analyses of VEGFR2 expression. Results: Real-time PCR and Western blot results showed that VEGFR2 expression signifcantly downregulated. This suppression was followed by inhibition of cell proliferation, reduction of viability, and induction of apoptosis in the cancer cells. Conclusion: These findings suggest that VEGFR2 has a role in cell proliferation and tumor growth. Accordingly, it is suggested that VEGFR2 can be a therapeutic target for controlling tumor growth and proliferation

Optimization and economic evaluation of modified coagulation–flocculation process for enhanced treatment of ceramic-tile industry wastewater

Abstract Enhanced treatment of ceramic-tile industry wastewater was investigated by modified coagulation–flocculation process using combination of poly-aluminum chloride (PAC) with anionic (A300), cationic polymer (C270) and nonionic polymers. The effects of pH, PAC coagulant dose alone and with polymers dose in various combinations was studied by jar tests. To compare the removal efficiencies of turbidity, total suspended solids (TSS), chemical oxygen demand (COD), and color at different levels, we run multivariate analysis of variance. Regarding the economic evaluation, we applied the incremental cost-effectiveness ratio. PAC had the best performance in pH 7 and in optimal dose of 400 mg/L; so that removal efficiency of wastewater turbidity, TSS, COD and color were 99.63%, 99.7%, 47.5% and 50.38%, respectively. The best removal efficiency for wastewater turbidity, TSS, COD and color were 99.87%, 99.89%, 87.5% and 93.02%, respectively which were obtained by combination of anionic polymer (1.5 mg/L) with PAC (300 mg/L). Furthermore, with combination of PAC + anionic + non-ionic polymers, the removal efficiency for wastewater turbidity, TSS, COD and color were 99.93%, 99.94%, 88% and 94.57%, respectively. The imposed cost for treating one cubic meter of ceramic-tile wastewater treatment by PAC + anionic and PAC + anionic and non-ionic polymers in comparison with PAC alone was reduced to 22.96% and therefore economically more affordable for the tile industry wastewater treatment