Development and validation of a LC-MS/MS method for the quantification of the checkpoint kinase 1 inhibitor SRA737 in human plasma.

Aim SRA737 is an orally active small-molecule inhibitor of checkpoint kinase 1 being investigated in an oncology setting. A HPLC-MS/MS method for quantifying plasma concentrations of SRA737 was validated.Methods & results Sample preparation involved protein precipitation with acetonitrile following addition of 13C15N-deuterated SRA737 as internal standard. A rapid and selective method was fully validated across a range of 5-20,000 ng/ml, exhibiting good sensitivity, overall precision (expressed as coefficient of variation) ≤8.0% and accuracy 96-102%. Consistently high recovery was observed, with no matrix effect and a lower limit of quantitation of 5 ng/ml.Conclusion A novel method for analyzing SRA737 in human plasma has been validated and is now being utilized for quantification of SRA737 in a Phase I trial

Liberali, cattolici e socialisti a Verona dalla fine dell\u2019Ottocento al Fascismo

Atti del convegno \u201cUna citt\ue0, un\u2019industria, una famiglia: i Galtarossa\u201d (tenutosi il 7.2.1997), organizzato da Associazione industriali e Osservatorio Provinciale del settore Metalmeccanico Fim Fiom Uilm di Veron

Presentazione

Quaderno 2 dell'Istituto veronese per la storia della Resistenz