In vivo DNA gene electro-transfer: a systematic analysis of different electrical parameters

Background Intramuscular plasmid injection followed by electroporation is an efficient method for gene therapy or vaccination. Several protocols have been described that give good transduction. levels with several reporter genes. Methods In this work we have explored the efficiency of gene delivery upon variation of the different electrical parameters such as pulse length frequency and voltage monitoring both on short- and long-term protein production. Results Having defined the best performing parameters, we have designed a short electric treatment that gives good levels of plasmid-encoded protein in different species such as mice, rabbits and monkeys. Copyright (C) 2005 John Wiley & Sons, Ltd

Genetic vaccination by gene electro-transfer in non-human primates

Muscle gene electro-transfer (GET) of plasmid DNA is a promising approach for gene therapy and genetic vaccination. Several protocols have been described which give good levels of gene transduction in small animals. However, to progress towards human applications, efficacy must be demonstrated in non-human primates. Here, we extensively explore several electrical and injection parameters in Rhesus monkeys and define a series of conditions for efficient vaccination