61 research outputs found
The effect of mesenchymal stem cells combined with platelet-rich plasma on skin wound healing
INTRODUCTION:
Mesenchymal stem cells (MSCs) are multipotent stem cells that have the potential of proliferation, high self-renewal, and the potential of multilineage differentiation. The differentiation potential of the MSCs in vivo and in vitro has caused these cells to be regarded as potentially appropriate tools for wound healing. After the burn, trauma or removal of the tumor of wide wounds is developed. Although standard treatment for skin wounds is primary healing or skin grafting, they are not always practical mainly because of limited autologous skin grafting.
EVIDENCE ACQUISITIONS:
Directory of Open Access Journals (DOAJ), Google Scholar, PubMed (NLM), LISTA (EBSCO), and Web of Science have been searched.
EVIDENCE SYNTHESIS:
For clinical use of the MSCs in wound healing, two key issues should be taken into account: First, engineering biocompatible scaffolds clinical use of which leads to the least amount of side effects without any immunologic response and secondly, use of stem cells secretions with the least amount of clinical complications despite their high capability of healing damage.
CONCLUSION:
In light of the MSCs' high capability of proliferation and multilineage differentiation as well as their significant role in modulating immunity, these cells can be used in combination with tissue engineering techniques. Moreover, the MSCs' secretions can be used in cell therapy to heal many types of wounds. The combination of MSCs and PRP aids wound healing which could potentially be used to promote wound healing
Schwann Cell-Like Cells: Origin and Usability for Repair and Regeneration of the Peripheral and Central Nervous System
Mesenchymal stem cells versus their conditioned medium in the treatment of ischemia/reperfusion injury: Evaluation of efficacy and hepatic specific gene expression in mice
Objective(s): The mechanisms underlying the beneficial effects of MSCs on hepatic I/R injury are still poorly described, especially the changes in hepatocyte gene expression. In this study, the effect of bone marrow-derived mesenchymal stem cells (BMSCs) and adipose tissue-derived mesenchymal stem cells (AMSCs) and their conditioned medium on hepatocyte gene expression resulted by I/R shock were investigated. Materials and Methods: Liver ischemia models were induced by clamping in experimental groups. Experimental groups received MSCs or conditioned medium treatments and the control group received Dulbecco's Modified Eagle Medium (DMEM). During 1, 24 hr, and 1 week after treatment, the serum levels of alanine aminotransferase (ALT), aspartate transaminase (AST) and lactate dehydrogenase (LDH) enzymes and tissue catalase activity (CAT) were measured. Gene expression of a number of hepatocyte-specific genes (Alb, Afp, and Ck8) and Icam-1 which is upregulated under inflammatory conditions were also evaluated in 5, 24 hr, and 1-week intervals after I/R insult. Results: In this study, liver enzymes showed a much more shift in the control group than treated groups and it was more noticeable 5 hr post-treatment. Moreover, gene expression pattern of the control group underwent changes after I/R injury. However, treated groups gene expression analysis met a steady trend after I/R insult. Conclusion: Our finding shows that stem cell treatment has better curative effects than conditioned medium. BMSCs, AMSCs or BMSC and AMSC-derived bioactive molecules injection have potential to be considered as a therapeutic approach for treating acute liver injury
Seeding nerve sutures with minced nerve-graft (MINE-G): a simple method to improve nerve regeneration in rats
Comparison of melatonin and curcumin effect at the light and dark periods on regeneration of sciatic nerve crush injury in rats
Being one of the acute clinical problems, peripheral nerve injury can bring about a number of consequences in-
cluding severe disability, redu
ced Quality of life (QOL) and immense costs. Currently, melatonin and curcumin
are widely applied because of their immunomodulatory, anti-inflammatory, neuro-protective and antioxidant prop-
erties. The present stud
y aims to compare the effects of melatonin and curcumin during light and dark periods on
sciatic nerve crush injury repair. Acco
rdingly, rats received IP injections
of curcumin (100 mg/kg) and melatonin
(10 mg/kg) over two periods of light (9:00 a.m.) and dark (9
:00 p.m.) for 4 weeks. In order to evaluate rats, func-
tional (walking track analysis and
electrophysiological measurements),
histomorphometric and gastrocnemius
muscle mass investigations were administered. No statis
tically significant difference
was identified between dark
and light curcumin groups while curcumin groups displayed better results than did melatonin groups. In addition,
dark melatonin group displayed better results than the light melatonin. On the whole, this study found that mela-
tonin and curcumin can be used to quicken neural recovery and help treat nerve injury. It was also found that better
neuroregeneration or nerve regeneration was induced when ra
ts were treated by melatonin during the dark period
while effects and injection time did not
correlate in curcum
in application
Engineered substrates with imprinted cell-like topographies induce direct differentiation of adipose-derived mesenchymal stem cells into Schwann cells
Effects of melatonin on the proliferation and differentiation of rat adipose-derived stem cells
Background: Osteogenesis driven by adipose-derived stem cells (ADSCs)
is regulated by physiological and pathological factors. Accumulating
evidence from in vitro and in vivo experiments suggests that
melatonin may have an influence on bone formation. However, little is
known about the effects of melatonin on osteogenesis, which thus
remains to be elucidated. This study was performed to determine whether
melatonin at physiological concentrations (0.01-10 nM) could affect the
in vitro proliferation and osteogenic differentiation of rat ADSCs.
Materials and Methods: ADSCs were isolated from the fat of adult rats.
After cell expansion in culture media and through three passages,
osteogenesis was induced in a monolayer culture using osteogenic medium
with or without melatonin at physiological concentrations (0.01-10 nM).
After four weeks, the cultures were examined for mineralization by
Alizarin Red S and von Kossa staining and for alkaline phosphatase
(ALP) activity using an ALP kit. Cell viability and apoptosis were also
assayed by 3-(4,
5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium
(MTT) assay and flow cytometry, respectively. Results: The results
indicated that at physiological concentrations, melatonin suppressed
proliferation and differentiation of ADSCs. These data indicate that
ADSCs exposed to melatonin, had a lower ALP activity in contrast to the
cells exposed to osteogenic medium alone. Similarly, mineral deposition
(calcium level) also decreased in the presence of melatonin. Flow
cytometry confirmed that cell growth had decreased and that the numbers
of apoptotic cells had increased. Conclusion: These results suggest
that the physiological concentration of melatonin has a negative effect
on ADSC osteogenesis
Correction: A Biosynthetic Nerve Guide Conduit Based on Silk/SWNT/Fibronectin Nanocomposite for Peripheral Nerve Regeneration
As a contribution to the functionality of nerve guide conduits (NGCs) in nerve tissue engineering, here we report a conduit processing technique through introduction and evaluation of topographical, physical and chemical cues. Porous structure of NGCs based on freeze-dried silk/single walled carbon nanotubes (SF/SWNTs) has shown a uniform chemical and physical structure with suitable electrical conductivity. Moreover, fibronectin (FN) containing nanofibers within the structure of SF/SWNT conduits produced through electrospinning process have shown aligned fashion with appropriate porosity and diameter. Moreover, fibronectin remained its bioactivity and influenced the adhesion and growth of U373 cell lines. The conduits were then implanted to 10 mm left sciatic nerve defects in rats. The histological assessment has shown that nerve regeneration has taken places in proximal region of implanted nerve after 5 weeks following surgery. Furthermore, nerve conduction velocities (NCV) and more myelinated axons were observed in SF/SWNT and SF/SWNT/FN groups after 5 weeks post implantation, indicating a functional recovery for the injured nerves. With immunohistochemistry, the higher S-100 expression of Schwann cells in SF/SWNT/FN conduits in comparison to other groups was confirmed. In conclusion, an oriented conduit of biocompatible SF/SWNT/FN has been fabricated with acceptable structure that is particularly applicable in nerve grafts
- …