Effects of 5f and 5g (10 μM) on the expression of phosphorylated (upper panel) and total (lower panel) stathmin protein in HeLa cells after incubation for 48 h, determined by western blot analysis.

<p>Results are mean values ± SEM of the data on two separate measurements, n = 6. ns indicates p > 0.05, *** indicates p < 0.001 as compared with the untreated control cells. Panels below are representative membrane pictures.</p

Induction of caspase-3, caspase-8 and caspase-9 activities after incubation with compounds 5f and 5g for 24 h.

<p>White, gray and black columns denote 3, 10 and 30 μM of the given agent. * and ** denote p < 0.05 and p < 0.01, respectively, as compared with the control condition.</p

Synthesis of steroidal 16α-triazoles by CuAAc.

<p>Synthesis of steroidal 16α-triazoles by CuAAc.</p

Expression of CDK1, cyclin B1, cyclin B2 and cdc25B at the mRNA level after incubation with 3 (gray columns) or 10 (black columns) μM of compounds 5f or 5g.

<p>*, ** and *** denote p < 0.05, p < 0.01 and p < 0.001, respectively, as compared with the control condition.</p

Effects of compounds 5f, 5g, 5h and 4i on the HeLa cell cycle distribution after incubation for 24 (left panels) and 48 h (right panels).

<p>Gray and black columns indicate 3 and 10 μM, respectively. * and ** denote p < 0.05 and p < 0.01, respectively, as compared with the control cells.</p

Fluorescent microscopy images of Hoechst 33258-PI double staining.

<p>Two separate pictures from the same field were taken for the two markers. MRC5 cells were treated with vehicle (control), or with <b>5f</b> and <b>5g</b> at the indicated concentrations. The blue fluorescence (left panels) indicates Hoechst 33258, and the red fluorescence (right panels) is a consequence of PI accumulation. The bar in the PI control picture indicates 100 μm.</p

Effects of compounds 5f and 5g on the Bax/Bcl-2 ratio after incubation of HeLa cells for 24 h.

<p>* and ** denote p < 0.05 and p < 0.01, respectively, as compared with the control condition.</p