1 research outputs found
A Switchable Site-Specific Antibody Conjugate
Genetic incorporation
of unnatural amino acids (UAAs) provides
a unique approach to the synthesis of site-specific antibody conjugates
that are homogeneous and better defined constructs than random conjugates.
Yet, the yield varies for every antibody, and the process is costly
and time-consuming. We have developed a switchable αGCN4-Fab
conjugate that incorporates UAA p-acetylphenylalanine. The GCN4 peptide
is used as a switch, and antibodies fused by GCN4 can direct the αGCN4-Fab
conjugate to target different cancer cells for diagnosis, imaging,
or therapeutic treatment. More importantly, this switchable conjugate
demonstrated an impressive potential for pretargeted imaging <i>in vivo</i>. This approach illustrates the utility of an orthogonal
switch as a general strategy to endow versatility to a single antibody
conjugate, which should facilitate the application of UAA-based site-specific
conjugates for a host of biomedical uses in the future