Evaluation of the cellular protection by novel spiropyrazole compounds in dopaminergic cell death

The loss of neurons is strongly correlated with aging and aging-associated disorders. In this study, cell viability assays and mitochondrial function were performed to evaluate the effect of new spiro-pyrazole derivatives, prepared from aldehydes and 3-amino-1-phenyl-2-pyrazolin-5-one, on neuroprotection in an in vitro model of dopaminergic cell death induced by 1-methyl-4-phenylpyridinium (MPP+). The percentages of neuroprotection by derivatives were found between 21.26% and 52.67% at selected concentrations (10-50 μM) with compound 4d exerting the best neuroprotective effect. The results show that the studied spiropyrazolones perform important roles in dopaminergic neuroprotection and can be used for potential new therapies in the treatment of neurodegenerative disorders including Parkinson's disease

An easy synthetic access to new pyrazole spiro derivatives from 3-amino-1-phenyl-2-pyrazolin-5-one

Pyrazole and pyrazolone moieties have shown important biological activities. For this reason, the synthesis of new derivatives has attracted interest from the scientific community. Herein we report the formation of two unexpected spiro compounds containing pyrazole moieties using a tandem process involving the initial condensation of 3-amino-1-phenyl-2-pyrazolin-5-one with benzaldehyde followed by dimerization