Effect of Adenoviral Transduction of Hepatic Stellate Cells with Adv5-optHGF-RFP on their Phenotype

© 2016, Springer Science+Business Media New York.Adenovirus is a promising gene delivery vector that has a high efficiency and relative simplicity of construction. These advantages make this system attractive for diverse research applications. In this project we transduced hepatics stellatecells (HSC) with adenoviral vector containing genes of hepatocytes growth factor (HGF) and red fluorescent protein (RFP) as a reporter (Adv5-optHGF-RFP), which let us to visualize the transduced cells. Further changes of phenotype were studied by real-time PCR and immunocytochemical staining. According to our results, most of the cells were transduced and demonstrated stable expression of RFP. Effectiveness of the transduction was also confirmed by a high expression of HGF (1200 times higher than in control HSC culture). Adenoviral transduction of HSC with HGF gene did not change their morphology, but stimulated expression of HSC marker desmin, leading to HSC activation (α-smooth muscle actin) and appearance of α-fetoprotein, one of the hepatoblasts markers. During the entire experiment, there were Ki-67+ cells, meaning that proliferation of transduced HSC was not affected. Thus, adenoviral transduction of HSC with Adv5-optHGF-RFP is a good gene delivery system with a stimulating effect on HSC

Analysis of nucleotide sequences of the <i>GT47</i> glycosyltransferase gene in rye cultivars differing in the content of water-soluble pentosans in grain

Rye (Secale cereale L.) is the most important crop in Russia, its grain quality depends on the content of water-soluble pentosans. The grain of rye cultivars with high content of water-soluble pentosans has good baking properties, but low fodder qualities. Methods of marker-assisted selection for this trait in rye remain undeveloped. For Triticeae, the content of pentosans in grain may be associated with the GT47 glycosyltransferase genes, but the genes of this family have not been identified in rye.  The aim of this study was amplification, sequencing, and search for single nucleotide substitutions or other mutations in the GT47 gene in various rye cultivars differing in the content of water-soluble pentosans in their grain and the viscosity of their aqueous extract. DNA from rye leaves was isolated by the standard CTAB method. Based on the nucleotide sequences of the bread wheat and barley GT47 genes, universal primers were selected, then a fragment of the open reading frames of the studied gene was amplified, and the nucleotide sequences were determined by automatic capillary sequencing.The population cultivars of rye, ‘Chulpan 7’ and ‘Podarok’, and F1 hybrids ‘KVS Aviator’, ‘KVS Magnifico’ and ‘KVS Eterno’ were analyzed. The highest content of pentosans and the highest kinematic viscosity of the aqueous extract were found in cv. ‘Chulpan 7’. The lowest values of these indicators were shown by the hybrid cv. ‘KVS Aviator’. The analysis of the nucleotide sequences of the GT47 gene revealed the presence of single-nucleotide substitutions in seven loci, in which the studied rye cultivars differed. Of these, the high-pentosan cv. ‘Chulpan 7’ and the low-pentosan cv, ‘KVS Aviator’ differed in three nucleotide substitutions: 159 (G/A), 204 (C/T), and 327 (G/A). It is suggested that these SNPs can be used for genotyping rye cultivars for the content of water-soluble pentosans in grain

Epidemiological Situation on Crimean-Congo Hemorrhagic Fever in the Russian Federation in 2012 and Prognosis for 2013

Represented is epidemiological evaluation of the morbidity rate as regards Crimean-Congo hemorrhagic fever (CCHF) in Russia in 2012. Summarized are the results of epizootiological surveillance over the territory of CHF natural focus in the South of European part of Russia. Forecast of CCHF epidemiological situation development for 2013 is made based on the epizootiological monitoring data

Native and Activated Hepatic Stellate Cells Stimulate Liver Regeneration in Rats After Partial Hepatectomy and 2-Acetylaminofluorene Injection

© 2016, Springer Science+Business Media New York.One the current challenges of modern hepatology is to find new approaches to stimulate liver regeneration and to find new methods for liver disease treatment. Cell therapies, which are based on using regional stem cells for disease treatment, are under active development. However, studies, devoted to their transplantation, are currently scarce. In recent years, hepatic stellate cells are considered to be hepatic stem cells. It is known that activated hepatic stellate cells can transdifferentiate into myofibroblasts and lead to liver fibrosis. The aim of our work was to study the influence of native and activated hepatic stellate cells in vivo by lead nitrate injection after transplantation into partial hepatectomized rats, which is considered to be a classical model to study liver regeneration. Injection of 2-acetylaminofluorene (AAF), which selectively eliminates hepatocyte proliferation, was used to understand the hepatic stellate cells role in liver regeneration process better. Our results suggest that transplanted native and activated hepatic stellate cells can differentiate into hepatocyte-like cells and positively influence liver regeneration without inducing liver fibrosis

Experience of the Ural federal university for implementation of international educational program in water conditioning and wastewater treatment

This paper briefly reports on the first international summer school on Traditional and Innovative Technologies of Water and Wastewater Treatment, which was held June 18 – July 29, 2018, Yekaterinburg, Russia as part of the project “UrFU Summer University”

Megasports events as an instrument for development adaptive (in focus of the XVIII Deaflympic winter games - 2015)

Nowadays the problems of disabled people and disability are one of the most important government problems, because this problem concerns a large number of people with disability. Disability is a social phenomenon that no country can avoid. One of the most essential directions of disabled people integration in our society and their successful physical recovery are physical culture and sport. It is just as important as rehabilitation with the help of education and work. Physical culture and sport for disabled are generally considered to be not only a method of physical rehabilitation, but also a method of social rehabilitation (social activity and personal achievements) - a permanentform of vital energy. The basic instruments ofdevelopment ofadaptive sports are examined in this article. Special attention is paid to sport events, particularly to the XVIII Deaflympic Winter Games, which were held in Hanty-Mansiysk, Russia in 2015

Deep eutectic solvent route synthesis of zinc and copper vanadate n-type semiconductors – mapping oxygen vacancies and their effect on photovoltage

Ternary metal oxides M2V2O7−δ (M = Zn and Cu) were synthesized by dissolving binary metal oxide precursors in an environmentally benign deep eutectic solvent (DES), which is a eutectic mixture of a hydrogen bond donor and acceptor, followed by annealing in an open crucible. The unique reaction environment provided by the evolved ammonia allows for stabilization of oxygen vacancies and reduced oxidation states of metal ions within an oxide matrix without the need for any post-treatment with flammable reducing agents. According to comprehensive characterization, including X-ray photoelectron spectroscopy (XPS), neutron powder diffraction, and UV-vis spectroscopy, oxygen deficiency is accompanied by reduced oxidation states of metal centers (Cu+ or V4+), resulting in oxides with mixed-valence metal oxidation states. The amount of oxygen vacancies can be tuned by changing the annealing temperature providing control over band gaps of ternary metal oxides and mid-gap states from reduced metal centers. All synthesized vanadates are n-type semiconductors based on negative photovoltages obtained from surface photovoltage spectroscopy (SPS). A decay of the photovoltage with increasing annealing temperatures is attributed to electron trapping and electron/hole recombination at V4+ and Cu+ mid-gap states. This work shows for the first time the impact of oxygen vacancies on the electronic structure of DES synthesized oxides for solar energy conversion applications.This article is published as Hong, Sangki, Rachel M. Doughty, Frank E. Osterloh, and Julia V. Zaikina. "Deep eutectic solvent route synthesis of zinc and copper vanadate n-type semiconductors–mapping oxygen vacancies and their effect on photovoltage." Journal of Materials Chemistry A 7, no. 19 (2019): 12303-12316. DOI: 10.1039/C9TA00957D. Copyright 2019 The Royal Society of Chemistry. Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0). Posted with permission

Study of the effect of narrow-leaved cypress extract on the properties of baking yeast

This article presents the results of the study of the beneficial properties of narrow-leaved rosebay willowherb (Chamerion angusti folium (L.) Holub) (the contents of vitamins, minerals and other substances which positively affect the human body). The process of extraction of a narrow-leaved spray from a dry plant raw material with an aqueous solution was looked at. The investigation of the concentration of narrow-leaved rosebay willowherb in an aqueous solution using a refractometer revealed that a 20 min. infusion shows the constant growth of dry matter in the extract. However after the 20 min. period the concentration of solids stays constant. Therefore, the optimum infusion time was set to 20 min. As a result of the study, the accelerated method of lifting the pressed yeast using an aqueous solution and a lemon grass extract revealed that, with the addition of the plant additive, the lifting power of the yeast decreased by 10.5%.This indicates that the products of narrow-leaved rosebay willowherb favorably affect yeast cells. Yeast suspensions from water and yeast and aqueous extract of narrow-leaved rosebay willowherb and yeast spray have been studied. After 60 min. of thermostating and further microscope study, with the determination of the concentration of yeast cells with the help of Goryaev's chamber, allowed to establish that the concentration of yeast cells in suspension with the extract of narrow-leaved rosebay willowherb exceeds the concentration in the control suspension by almost 1.5 times. This phenomenon is caused by the stimulating effect of the individual components that make up the narrow-leaved rosebay willowherb (sugars, vitamins, minerals and other components), which are an additional source of energy. It is proposed to use the spray extract of the narrow-leaved rosebay willowherb as a raw material in the preparation of bakery products for preventive purposes

Synthesis of SrTiO3 and Al-doped SrTiO3via the deep eutectic solvent route

SrTiO3 and aluminum-doped SrTiO3 are synthesized by calcination of metal salts dissolved in a deep eutectic solvent (DES) without any post-synthesis treatment. The DES used is the eutectic mixture of choline chloride (hydrogen bond acceptor) and malonic acid (hydrogen bond donor). Titanium(IV) oxide bis(2,4-pentanedionate) is utilized as the non-volatile, easy-to-handle, DES-soluble titanium precursor. The ammonia gas evolved during the calcination process provides a reducing atmosphere, resulting in the formation of Ti3+ and oxygen vacancies within the SrTiO3 matrix. According to UV-Vis spectroscopy and X-ray photoelectron spectroscopy, the amount of Ti3+ species and oxygen vacancies (VO) in the synthesized perovskite can be tuned by varying the duration of the calcination process and by adding Al3+ dopants. Solid state 27Al NMR spectroscopy and powder X-ray diffraction confirm the doping of aluminum into the octahedral site of the perovskite structure. Surface photovoltage spectroscopy confirms that Al3+ dopants can eliminate Ti3+ defects in Al-doped SrTiO3. Ultraviolet illumination experiments in water and aqueous methanol show that SrTiO3 and aluminum-doped SrTiO3, after modification with RhxCr2−xO3 or Pt co-catalysts, evolve small amounts of H2 (EQE of 0.0113–0.0173% at 375 nm) with only traces of O2 detected. The lack of photocatalytic activity is attributed to rapid electron-hole recombination in the oxygen vacancy-rich materials and to the lack of crystal facets that could aid charge separation.This article is published as Adeyemi, Adedoyin N., Amrit Venkatesh, Chengcan Xiao, Zeqiong Zhao, Ying Li, Tori Cox, Dapeng Jing, Aaron J. Rossini, Frank Osterloh, and Julia V. Zaikina. "Synthesis of SrTiO 3 and Al-doped SrTiO 3 via deep eutectic solvent route." Materials Advances (2022). DOI: 10.1039/D2MA00404F. Copyright 2022 The Author(s). Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). Posted with permission. DOE Contract Number(s): AC02-07CH11358; SC0015329

Sensory and physic-chemical properties of wheat bread prepared with apple pomace

The influence of secondary apple raw materials (frozen apple pomace) on organoleptic and physicochemical indicators of the quality of wheat bread has been studied. The traditional recipe of wheat bread was taken as a basis. In this recipe for the sample of bread No. 1, a part of the premium wheat flour was replaced with 25% rice flour and 10% frozen apple pomace. In the sample of bread No. 2, a part of the premium wheat flour was replaced with 12.5% rice flour and 10% fresh frozen apple pomace. For the organoleptic assessment, a five-point scale for assessing the quality of bread was developed, in which the maximum number of points up to5 was assigned to each indicator. According to the results of the study of the organoleptic indicators of the quality of the developed samples of bread using a point scale, it was found that they exceeded the control sample. The best was the sample with fresh frozen apple pomace10% and rice flour in the amount of 12.5%. In terms of physical and chemical indicators, the developed samples of wheat bread enriched with apple raw materials meet the regulatory requirements