Visual Assay of Glutathione in Vegetables and Fruits Using Quantum Dot Ratiometric Hybrid Probes

Future food safety monitoring with simple, fast, and visual methods has become increasingly important. Accordingly, this work was designed to construct a new-style dual-emission ratiometric fluorescent probe (CdSe@SiO₂@CdTe) for visual assay of glutathione (GSH) with a “turn on” strategy. After adding Hg²⁺, the red fluorescence of the outer CdTe quantum dots (QDs) was quenched through both electron transfer and ion-binding processes. Upon the addition of GSH, the red fluorescence occurred again owing to the strong affinity between GSH and Hg²⁺, whereas the inner green fluorescence of CdSe QDs was unchanged, leading to a clearly recognizable fluorescence color change (from green to orange–red). In the concentration range from 0.1 to 10 μM, the relative fluorescence intensity ratios (<i>I</i>₆₁₉/<i>I</i>₅₃₅) showed an excellent linear correlation with the concentration of GSH, and the detection limit was as low as 42 nM under optimal conditions. Meanwhile, the ratiometric hybrid probes were successfully applied for direct visual sensing GSH in real vegetable and fruit samples

Plasma Lipidomics Investigation of Hemodialysis Effects by Using Liquid Chromatography–Mass Spectrometry

Chronic kidney disease (CKD) has been a global health problem that has a great possibility of being developed into uremia in the end. Hemodialysis (HD) is the most commonly used strategy for treating uremic patients; however, the patients still have a high risk of suffering various complications. It is well recognized that lipid disorder usually occurs in maintenance HD patients. To systemically study the effects of HD on lipid metabolism associated with uremia, we employed an ultraperformance liquid chromatography–quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS)-based lipidomics method. A total of 87 human plasma samples from patients with prehemodialysis (pre-HD)/posthemodialysis (post-HD) treatment and the healthy controls were enrolled in the study. As compared with pre-HD patients, many plasma lipids showed significant changes (<i>p</i> < 0.05) in patients receiving HD therapy. Specifically, sum of free fatty acids (FFA) as well as saturated FFA and eicosanoids and sums of lyso-phosphatidylinositols and lyso-phosphatidylethanolamines, FFA 16:1/FFA 16:0, and FFA 18:1/FFA 18:0 were obviously higher in the pre-HD group than in the controls while they were significantly lower in patients after HD. These results indicated that UPLC-Q-TOF/MS-based lipidomics is a promising approach to investigate lipid alterations in relation to uremia and it is helpful to understand complex complications involved in HD patients

Dual-Metal Centered Zirconium–Organic Framework: A Metal-Affinity Probe for Highly Specific Interaction with Phosphopeptides

The highly specific affinity between probes and phosphopeptides is the fundamental interaction for selective identification of phosphoproteomes that uncover the mechanisms of signal transduction, cell cycle, enzymatic regulation, and gene expression in biological systems. In this study, a metal-affinity probe possessing both interactions of metal oxide affinity chromatography (MOAC) and immobilized metal ion affinity chromatography (IMAC) was facilely prepared by immobilizing zirconium­(IV) on a zirconium–organic framework of UiO-66-NH₂, which holds dual-metal centers of not only the inherent Zr-O cluster but also the immobilized Zr­(IV) center. This dual-metal centered zirconium–organic framework (DZMOF) demonstrates as a highly specific metal-affinity probe toward the extraction of phosphopeptides due to the metal-affinity interactions of MOAC and IMAC toward either mono-phosphorylated or multi-phosphorylated peptides. The binding energies of zirconium 3d_5/2 and 3d_3/2 in this DZMOF are 183.07 and 185.47 eV, respectively, which are higher than those of the intact UiO-66-NH₂ (182.84 and 185.17 eV, respectively), confirming the higher metal-affinity interaction between the DZMOF and phosphopeptides. This high metal-affinity probe presents an unprecedented strong performance in anti-nonspecific interference during the capturing of phosphopeptides of β-casein with the molar ratio of β-casein vs bovine serum albumin up to ca. 1:5000. The enrichment of phosphopeptides from a human saliva sample by DZMOF further confirms the great potential of DZMOF in the extraction of low-abundance phosphopeptides for real complex biological samples